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Muscle Response to High Intensity Interval Resistance Training (HIIRT) (HIIRT)

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ClinicalTrials.gov Identifier: NCT04321772
Recruitment Status : Completed
First Posted : March 25, 2020
Last Update Posted : March 25, 2020
Sponsor:
Collaborator:
University of Roma La Sapienza
Information provided by (Responsible Party):
Antonio Paoli, University of Padova

Brief Summary:
Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Condition or disease Intervention/treatment Phase
Exercise Resistance Training Other: High Intensity Interval Resistance Training Other: Traditional Resistance Training Not Applicable

Detailed Description:
Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 9 participants
Allocation: Randomized
Intervention Model: Crossover Assignment
Intervention Model Description: Same subjects serve as both the test and control arms which allows for within-subject comparison. Using a within-person design, participants trained each lower limb on separate days under two different training stimulus (HIIRT) and traditional training (TRT)
Masking: Single (Outcomes Assessor)
Masking Description: The samples analysed were masked by a number without any indication of the arm.
Primary Purpose: Basic Science
Official Title: Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training
Actual Study Start Date : February 1, 2012
Actual Primary Completion Date : May 15, 2012
Actual Study Completion Date : February 10, 2013

Resource links provided by the National Library of Medicine


Arm Intervention/treatment
Experimental: High Intensity Interval Resistance Training (HIIRT)
HIIRT technique consisted of three sets of: 6 repetitions at 80% 1RM (1 repetition maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets.
Other: High Intensity Interval Resistance Training
Subjects trained one leg with HIIRT

Active Comparator: Traditional Resistance Training (TRT)
TRT protocol performed 3 series of 15 repetitions at 60% 1RM with 75" of rest between sets.
Other: Traditional Resistance Training
Subjects trained the other leg with HIIRT




Primary Outcome Measures :
  1. pAMPKThr172 in skeletal muscle tissue measured by immunoblot [ Time Frame: 24 hours ]
    measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy

  2. pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot [ Time Frame: 24 hours ]
    measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy

  3. pS6Ser235/236 in skeletal muscle tissue measured by immunoblot [ Time Frame: 24 hours ]
    measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy

  4. p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot [ Time Frame: 24 hours ]
    measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy

  5. ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot [ Time Frame: 24 hours ]
    ACC phosphorylation in skeletal muscle tissue taken by biopsy

  6. IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    IGF-1 mRNA in skeletal muscle tissue taken by biopsy

  7. IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hoursn ]
    IGF-1ea mRNA in skeletal muscle tissue taken by biopsy

  8. MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    MGF mRNA in skeletal muscle tissue taken by biopsy

  9. STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hoursn ]
    STARS mRNA in skeletal muscle tissue taken by biopsy

  10. Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    Myogenin mRNA in skeletal muscle tissue taken by biopsy

  11. IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    IL-6 mRNA in skeletal muscle tissue taken by biopsy

  12. PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    PGC1a4 mRNA in skeletal muscle tissue taken by biopsy

  13. PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    PGC1a mRNA in skeletal muscle tissue taken by biopsy

  14. Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    Atrogin mRNA in skeletal muscle tissue taken by biopsy

  15. Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    Beclin mRNA in skeletal muscle tissue taken by biopsy

  16. Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [ Time Frame: 24 hours ]
    Myostatin mRNA in skeletal muscle tissue taken by biopsy



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Ages Eligible for Study:   20 Years to 30 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • moderate active
  • experience with resistance training

Exclusion Criteria:

  • history of recent myocardial infarction,
  • severe cardiac arrhythmia,
  • unstable angina,
  • poorly controlled hypertension,
  • poorly controlled diabetes mellitus,
  • frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT04321772


Locations
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Italy
Nutrition and Exercise Lab, DSB, University of Padova
Padova, Italy, 35131
Sponsors and Collaborators
University of Padova
University of Roma La Sapienza
Investigators
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Study Director: Antonio Paoli, MD University of Padova

Publications:
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Responsible Party: Antonio Paoli, Full Professor, University of Padova
ClinicalTrials.gov Identifier: NCT04321772    
Other Study ID Numbers: HIIRT12
First Posted: March 25, 2020    Key Record Dates
Last Update Posted: March 25, 2020
Last Verified: March 2020
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
Keywords provided by Antonio Paoli, University of Padova:
resistance training
recovery
muscle hypertrophy
muscle signalling