Potential Impact of Polycystic Ovarian Syndrome on Protein Modifications and Accumulation
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|ClinicalTrials.gov Identifier: NCT02105428|
Recruitment Status : Completed
First Posted : April 7, 2014
Last Update Posted : May 17, 2016
|Condition or disease||Intervention/treatment||Phase|
|Polycystic Ovarian Syndrome (PCOS) Insulin Resistance Overweight Obese||Behavioral: Aerobic Exercise Training||Not Applicable|
|Study Type :||Interventional (Clinical Trial)|
|Actual Enrollment :||44 participants|
|Intervention Model:||Parallel Assignment|
|Masking:||None (Open Label)|
|Primary Purpose:||Basic Science|
|Official Title:||Potential Impact of Polycystic Ovarian Syndrome on Protein Modifications and Accumulation|
|Study Start Date :||July 2010|
|Actual Primary Completion Date :||December 2015|
|Actual Study Completion Date :||December 2015|
No Intervention: Sedentary Control
Participants will be randomized to an exercise training program or sedentary control group. The sedentary will not perform any structured physical activity or exercise. Participants will be encouraged to maintain their sedentary lifestyle and will be monitored by an accelerometer to track physical activity.
Experimental: Aerobic Exercise Training
Participants will perform 12-weeks of aerobic exercise training
Behavioral: Aerobic Exercise Training
A progressive increase in duration, frequency and intensity so the last 8 weeks of exercise are performed for 60 min, 5 days per week and at 80% of aerobic capacity. The duration includes a 5 minute warm up and 5 minute cool down. All exercise will be performed on a stationary bicycle (i.e., cycle ergometer).
- Change in insulin sensitivity [ Time Frame: Measured before and after 12 weeks ]To determine the effect of 12 weeks of aerobic exercise training or sedentary behavior on insulin sensitivity measured by a 3 hour hyperinsulinemic-euglycemic clamp and response to a mixed-meal.
- Protein modifications and accumulation [ Time Frame: Before and after 12 weeks ]Alterations in protein modifications and accumulation will be measured by a novel methodology developed within our laboratory. Infusion of stable amino acid isotopes will be used to measure the accumulation of proteins before and after 12 weeks of aerobic exercise training or sedentary behavior. Plasma and muscle will be resolved using a two dimensional gel electrophoresis which will allow the identification of protein modifications and age.
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02105428
|United States, Minnesota|
|Rochester, Minnesota, United States, 55905|