Stem Cell Collection for Adult Volunteers
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|ClinicalTrials.gov Identifier: NCT00033774|
Recruitment Status : Recruiting
First Posted : April 10, 2002
Last Update Posted : August 7, 2020
This study will examine the development of stem cells (very immature cells produced by the bone marrow) and their potential to change into cells of other organ types. These cells will be studied for their potential use in creating replacement tissue for diseases ranging from diabetes to Parkinson s.
Healthy volunteers 18 years of age or older may be eligible for this study. Candidates will be screened with a medical history, physical examination, and blood tests.
Participants will undergo a process called 'stem cell mobilization and apheresis' to collect bone marrow stem cells. For five days before the collection they will receive injections of a hormone called G-CSF, which stimulates release of stem cells from the bone marrow into the bloodstream. On the fifth day of the injections, stem cells will be collected through apheresis. For this procedure, blood is collected through a catheter (plastic tube) placed in an arm vein and directed into a cell separator machine. There, the white cells and stem cells are separated from the other blood components through a spinning process and collected in a bag inside the machine. The rest of the blood is returned to the donor through a catheter in the other arm.
|Condition or disease|
|Study Type :||Observational|
|Estimated Enrollment :||99999 participants|
|Official Title:||Peripheral Blood Stem Cell Collection From Adult Volunteers|
|Actual Study Start Date :||April 29, 2002|
Intervention(medication):Volunteers will undergo mobilization with G-CSF for 5 consecutive days followed by large volume apheresis on the 5th day of G-CSF injection.
- To explore the control of normal differentiation of adult hematopoietic stem cells collected from the peripheral blood after G-CSF mobilization. [ Time Frame: ongoing ]Collection and storage of hematopoietic stem cells will allow in vitro studies to explore the control of normal hematopoiesis stem cell differentiation. In some cases, steady state (without G-CSF mobilization) peripheral blood mononuclear cells will be collected to serve as controls. The hematopoietic stem cells will be cultured under a variety of conditions designed to mimic normal hematopoietic stem cell terminal differentiation. The hematopoietic stem cells will also be transplanted into immunodeficient mice, a surrogate stem cell assay, to further study in vivo differentiation potential. Populations of cultured cells will be analyzed for genes involved in the process ofnormal differentiation. Following collection and processing, identifiers will be stripped from the samples prior to their distribution to the investigators with retention of only the gender of the donor for purposes of cell tracking.
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00033774
|Contact: John F Tisdale, M.D.||(301) email@example.com|
|United States, Maryland|
|National Institutes of Health Clinical Center, 9000 Rockville Pike||Recruiting|
|Bethesda, Maryland, United States, 20892|
|Contact: For more information at the NIH Clinical Center contact Office of Patient Recruitment (OPR) 800-411-1222 ext TTY8664111010 firstname.lastname@example.org|
|Principal Investigator:||John F Tisdale, M.D.||National Heart, Lung, and Blood Institute (NHLBI)|