The Role of Endothelin-1 in Sickle Cell Disease
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|ClinicalTrials.gov Identifier: NCT02712346|
Recruitment Status : Active, not recruiting
First Posted : March 18, 2016
Last Update Posted : July 17, 2019
|Condition or disease||Intervention/treatment||Phase|
|Sickle Cell Anemia||Drug: Ambrisentan Drug: Placebo||Phase 1|
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The purpose of this study is to test the hypothesis that endothelin antagonist (ETA) receptor blockade using ambrisentan is safe, tolerable, and improves kidney function/albuminuria in patients with sickle cell disease (SCD). The investigators anticipate a reduction in proteinuria, a decrease in tricuspid regurgitation jet (TRJ) velocity, reduction in inflammatory markers, improvement in forearm blood flow, and improvement in nociception/pain.
The primary efficacy endpoint was chosen as the effect of ETA receptor blockade on the reduction of microalbuminuria based upon findings in diabetic nephropathy, which has a similar pathogenesis to sickle nephropathy. A 30% reduction in proteinuria is rather conservative and realistic based upon the results of studies of ETA receptor blockade in diabetic nephropathy that consistently resulted in 40-45% reduction in proteinuria. Pre-clinical data has shown that changes can be detected in urinary nephrin excretion before overt proteinuria is observed in a model of chronic ET-1 elevation. Furthermore, tubular injury that can occur as a result of an intrarenal vaso-occlusive crisis (VOC) should be expected to increase the level of renal tubular injury markers, neutrophil gelantinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), and netrin. Treatment with ambrisentan would be expected to attenuate these changes. Dr. Jennifer Pollock, has extensive experience with all of the biomarker assays including ET-1. Dr. Pollock runs bio-analytical core labs for two PO1s and has published extensively on these and similar methods in human and animal samples.
Additional rationale for this project is based on recent 24-hour urine results for creatinine clearance and total protein excretion from 97 patients with sickle cell anemia (Hb SS) and sickle beta zero thalassemia (SB0-thalassemia) followed in the Augusta Sickle Cell Clinic. These patients ranged in age from 19-63 years (52 females, 45 males). Of these, 17 (18%) had microalbuminuria as defined by a 24-hour albumin excretion of 150-300 mg, 34 (36%) had macroalbuminuria (>300 mg albumin excretion/24-hours), thus 54% of the patients had microalbuminuria or macroalbuminuria and only 46% were normo albuminuric. 58 patients (58%) were on chronic hydroxyurea therapy. 9 patients were on angiotensin converting enzyme inhibitors (ACEi) and 20 were on angiotensin receptor blockers (ARBs) (total 30%). 55 patients (57%) had glomerular hyperfiltration (creatinine clearance of >120 ml/min). There was an age related decline in the glomerular filtration rate (GFR) observed. The number of patients with normo albuminuria shows a sharp decline by age indicative of the progression of sickle nephropathy. Recent studies of pain in SCD have led to a change in perception. Approximately 50% of the patients reported experiencing daily, chronic pain. In addition, issues related to centralization and neuropathic pain in this patient population has begun to gain increasing attention. A large body of evidence suggests that endothelin-1 plays an important role in enhancing pain stimuli and nociception in various conditions. This is mediated by ETA receptor. Berkeley SCD transgenic mice do have hyperalgesia as shown in the rationale preliminary data section of Aim 2 and ETA receptor blockade reverses this hyperalgesia towards normal. As part of an ongoing National Institute on Minority Health and Health Disparities (NIMHD) funded study of pain and its genetic correlates (study reference: 1 P20 MD003383-01), the investigators have found that SCD patients display significant hyperalgesia as measured by pressure pain algometer testing. The testing was done at three different anatomic sites in patients (n=38) and controls (n=20) and showed that SCD patients perceived pain at significantly lower pressures compared to controls (masseter: 157.7 kilopascal (kPa) for patients, 214.4 kPa for controls, p=0.017; ulna: 299.1 kPa for patients, 477.5 kPa for controls, p=0.0018; and trapezius: 290.1 kPa for patients, 462.8 kPa for controls, p=0.02). These data suggest that hyperalgesia is present in the vast majority of SCD patients and will constitute an objective parameter to monitor during the study.
Protocol-required assessments performed at every study visit include: physical exam, vital signs, con medication review, study drug accountability, adverse events review, pain diary completion, and pregnancy testing if applicable.
Protocol-required assessments performed at specified study visits include: collection of blood and urine for safety and efficacy testing; electrocardiogram (ECG); echocardiogram (echo); Quantitative Sensory Testing (pressure pain threshold via algometer, cutaneous mechanical pain via monofilament, and thermal testing via Q-Sense Small Fiber Test); transcranial Doppler (TCD); forearm blood flow measurement; skin blood flow measurement; and completion of quality of life questionnaires.
Description of Procedures:
Sensory Testing: Patients will also undergo sensory testing at baseline and at the end of the study period. Sensory testing will include: 1) Pressure pain threshold will be measured by a hand-held, digital algometer as described by Fillingim et al at three different anatomical sites: center of right upper trapezius, right masseter, and the right ulna; 2) Cutaneous mechanical pain testing- Assessment of temporal summation of mechanical pain will be done using nylon monofilaments tested on the dorsum of the hand.; and 3) Thermal sensory testing will be performed using Q-Sense Small Fiber Test, which offers a scientifically validated measure of warm, cool and heat-pain thermal sensory thresholds.
Pain Diaries/Questionnaires: Patients will be asked to complete a daily pain diary as described in the PISCES study. The evaluation of pain diaries will be performed by Dr. Robert Gibson and the research staff. In addition, investigators will also incorporate two pre/post measures that will examine the effects of pain on functional performance and quality of life. The Assessment of Motor and Process Skills (AMPS) is a well-established functional assessment. This assessment has demonstrated the ability to discriminate between healthy controls and those with wide spread chronic pain. It also demonstrates the ability to measure change following intervention. The investigators will also measure patient's quality of life with the Adult Sickle Cell Quality of Life Measurement Information System (ASCQ-Me). This quality of life assessment has been specifically developed for sickle cell populations. Dr. Robert Gibson has training and experience with the ASCQ-Me and is certified to administer the AMPS.
Echocardiogram for tricuspid regurgitation jet (TRJ) Velocity Measurement: A transthoracic echocardiogram for the measurement of TRJ velocity and pulmonary artery systolic pressure will be performed at baseline (unless obtained clinically within 6 months of Day 1) and at the end of the study.
Electrocardiogram: An electrocardiogram (ECG) is a test that measures the electrical activity of the heart. An ECG will be done as a safety measure at baseline, Day 8 visit, and end of study, although ambrisentan is not known to prolong corrected QT interval (QTc).
Forearm Blood Flow Measurement: Brachial artery flow-mediated dilation (FMD) will be performed in accordance to the recent tutorial for ultrasound assessment of FMD. Patients will be tested under fasting conditions in a controlled environment at baseline and at the end of the study period.
Skin Blood Flow Measurement: Skin blood flow will be measured non-invasively using a low frequency laser imaging camera and/or a small skin probe. Ring-shaped probes that are about 1.5 inches wide will be placed on the forearm with sticky tape. One ring will be filled with approximately 2 ml (about 1/2 teaspoon) of water for local heat and the others will be used for iontophoresis. For local heat, investigators will heat the water in the ring to between 42°C (107°F) and 44°C (111°F) and wait approximately 30 minutes for the increase in skin blood flow to become stable. Iontophoresis is a technique that delivers a vasoactive drug about 1 mm deep into the skin using a low level electrical current. The advantage of this technique is that it is non-invasive and the drug does not go into the body, it just remains at the surface of the skin. We will use a 1-2% solution of either acetylcholine, sodium nitroprusside, or N-nitro-L-arginine methyl ester (L-NAME) (vasoactive substances) to increase or decrease skin blood flow. Multiple short currents (10-180 seconds) will be performed approximately 1 minute apart. Investigators will monitor skin blood flow through the ring during the forearm arterial blood flow test described above as well as during local heat and iontophoresis.
Blood and Urine Measurement: Lab tests to be performed for safety/ efficacy assessments at specified study visits include complete blood count (CBC), complete metabolic panel (CMP), spot urine for albumin-creatinine ratio, 24-hour urine, pregnancy testing, biomarkers, and inflammatory markers.
Transcranial Doppler (TCD): Pre- and post-treatment TCD will be performed to assess velocity of cerebral blood flow. This non-invasive test is performed while the subject is lying down and involves use of sound waves to assess macro-circulation in the head. A transducer is used to hear and record the results.
|Study Type :||Interventional (Clinical Trial)|
|Actual Enrollment :||26 participants|
|Intervention Model:||Parallel Assignment|
|Masking:||Triple (Participant, Care Provider, Investigator)|
|Official Title:||The Role of Endothelin-1 in Sickle Cell Disease|
|Study Start Date :||September 2015|
|Actual Primary Completion Date :||May 30, 2019|
|Estimated Study Completion Date :||November 30, 2019|
Ambrisentan 5 mg PO daily
Ambrisentan 5 milligrams a day or a placebo (sugar pill) for twelve weeks
Other Name: Letairis
Placebo Comparator: Placebo
One inactive pill PO daily
One inactive pill daily for twelve weeks
Other Name: "Sugar Pill"
- Safety and Tolerability of ambrisentan in patients with sickle cell disease measured by physical exam, vital signs, blood and urine testing, ECG (specified visits), concomitant medication review, adverse events review [ Time Frame: Day 1 (Baseline) through Day 113 ]
- Efficacy of ambrisentan in improving kidney function in patients with sickle cell disease measured by blood [ Time Frame: Day 1 (Baseline), Day 15, Day 29, Day 57, Day 85, and Day 113 ]measured by blood
- Efficacy of ambrisentan in decreasing TRJ velocity [ Time Frame: Day 1 (Baseline) and at the end of the 12 week treatment period ]measured by echocardiogram
- Efficacy of ambrisentan in decreasing inflammation [ Time Frame: Day 1 (Baseline) through Day 113 ]measurement of inflammatory markers in blood
- Efficacy of ambrisentan in improving micro-circulation [ Time Frame: Day 1 (Baseline) and at the end of the 12 week treatment period ]measured by forearm and skin blood flow measurements
- Efficacy of ambrisentan in improving macro-circulation [ Time Frame: Day 1 (Baseline), and at the end of the 12 week treatment period ]measured by Transcranial Doppler (TCD)
- Efficacy of ambrisentan in improving nociception/pain [ Time Frame: Day 1 (Baseline), and at the end of the 12 week treatment period ]performance of quantitative sensory testing
- Efficacy of ambrisentan in improving kidney function in patients with sickle cell disease measured by urine testing for microalbuminuria/proteinuria [ Time Frame: Day 1 (Baseline), Day 15, Day 29, Day 43, Day 57, Day 71, Day 85, and Day 113 ]measured by urine testing for microalbuminuria/proteinuria
- Efficacy of ambrisentan in improving nociception/pain [ Time Frame: Day 1 (Baseline) through the 12 week treatment period ]assessment of pain diaries/questionnaires
- Efficacy of ambrisentan in improving nociception/pain [ Time Frame: Day 1 (Baseline) through the 12 week treatment period ]assessment of adverse events
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02712346
|United States, Georgia|
|Augusta, Georgia, United States, 30912|
|Principal Investigator:||Abdullah Kutlar, MD||Augusta University|