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Investigation of Dietary Nitrate Effects in Hypertension-induced Target Organ Damage (NITRATE-TOD)

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ClinicalTrials.gov Identifier: NCT03088514
Recruitment Status : Recruiting
First Posted : March 23, 2017
Last Update Posted : September 7, 2020
Sponsor:
Collaborator:
Barts & The London NHS Trust
Information provided by (Responsible Party):
Amrita Ahluwalia, Queen Mary University of London

Tracking Information
First Submitted Date  ICMJE March 9, 2017
First Posted Date  ICMJE March 23, 2017
Last Update Posted Date September 7, 2020
Actual Study Start Date  ICMJE March 23, 2017
Estimated Primary Completion Date March 2022   (Final data collection date for primary outcome measure)
Current Primary Outcome Measures  ICMJE
 (submitted: March 16, 2017)
  • Left ventricular hypertrophy regression [ Time Frame: 4 months ]
    change in LV mass as assessed using cardiac magnetic resonance imaging
  • Pulse wave velocity [ Time Frame: 4 months ]
    non-invasive measures of arterial stiffness
  • Central blood pressure [ Time Frame: 4 months ]
    non-invasive measure of central blood pressure
Original Primary Outcome Measures  ICMJE Same as current
Change History
Current Secondary Outcome Measures  ICMJE
 (submitted: March 16, 2017)
  • Flow mediated dilatation (FMD) [ Time Frame: 4 months ]
    non-invasive measure of endothelial function using ultrasound
  • Brachial blood pressure [ Time Frame: 4 months ]
    brachial blood pressure
  • Change in plasma nitrate levels [ Time Frame: 4 months ]
    assessed by chemiluminescence
  • Change in plasma nitrite levels [ Time Frame: 4 months ]
    assessed by chemiluminescence
  • Change in nitric oxide activity (cGMP) [ Time Frame: 4 months ]
    nitric oxide activity measured by determining plasma concentrations of cyclic guanosine monophosphate (cGMP)
Original Secondary Outcome Measures  ICMJE Same as current
Current Other Pre-specified Outcome Measures Not Provided
Original Other Pre-specified Outcome Measures Not Provided
 
Descriptive Information
Brief Title  ICMJE Investigation of Dietary Nitrate Effects in Hypertension-induced Target Organ Damage
Official Title  ICMJE Investigation of Dietary Nitrate Effects in Hypertension-induced Target Organ Damage
Brief Summary This study aims to determine whether dietary inorganic nitrate (in beetroot juice) is able to reduce overall thickening of the heart (left ventricular hypertrophy or LVH) and stiffness of the arteries when given to patients with persistently raised blood pressure (hypertension). Half the patients will receive the beetroot juice containing inorganic nitrate and half will receive beetroot juice from which the inorganic nitrate has been removed. The volunteers will take the juice every day for 4 months.
Detailed Description

In hypertension persistent raised blood pressure (BP) is associated with endothelial dysfunction, arterial stiffness and left ventricular (LV) remodeling that are key phenomena associated with the pathogenesis and complications of hypertension.

One of the main substances that the healthy endothelium produces that is responsible for maintaining the patency of blood vessels is nitric oxide (NO). In hypertension, one of the key pathogenic effects is the dysfunction of the endothelium characterized by a decrease in ability to generate nitric oxide (NO). Previous studies have shown that dietary inorganic nitrate supplementation lowers blood pressure (Kapil et al. 2015), however, whether this approach might also improve endothelial function and LV remodeling is unknown. The effects of inorganic nitrate are due to its conversion in the body to inorganic nitrite and thereafter to NO.

This study will assess the effects of dietary inorganic nitrate on LVH using cardiac magnetic resonance imaging (NITRATE-LVH arm). In addition, the effects of dietary inorganic nitrate on central aortic blood pressure, arterial stiffness using pulse wave velocity and endothelial function using flow mediated dilatation will be evaluated (NITRATE-CBP arm).

Study Type  ICMJE Interventional
Study Phase  ICMJE Phase 2
Study Design  ICMJE Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
Primary Purpose: Treatment
Condition  ICMJE Hypertension
Intervention  ICMJE Dietary Supplement: Beetroot juice
Beetroot juice (70ml daily) with or without inorganic nitrate
Study Arms  ICMJE
  • Experimental: NITRATE-LVH intervention
    70ml of beetroot juice (approximately 6-8 mmol of inorganic nitrate) once a day for 4 months
    Intervention: Dietary Supplement: Beetroot juice
  • Placebo Comparator: NITRATE-LVH placebo
    70ml of beetroot juice (no inorganic nitrate) once a day for 4 months
    Intervention: Dietary Supplement: Beetroot juice
  • Experimental: NITRATE-CBP intervention
    70ml of beetroot juice (approximately 6-8 mmol of inorganic nitrate) once a day for 4 month
    Intervention: Dietary Supplement: Beetroot juice
  • Placebo Comparator: NITRATE-CBP placebo
    70ml of beetroot juice (no inorganic nitrate) once a day for 4 months
    Intervention: Dietary Supplement: Beetroot juice
Publications *

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruitment Information
Recruitment Status  ICMJE Recruiting
Estimated Enrollment  ICMJE
 (submitted: March 16, 2017)
160
Original Estimated Enrollment  ICMJE Same as current
Estimated Study Completion Date  ICMJE March 2022
Estimated Primary Completion Date March 2022   (Final data collection date for primary outcome measure)
Eligibility Criteria  ICMJE

Inclusion Criteria:

  1. Patients will be enrolled following an informed consent. The subject will be able to understand and comply with protocol requirements, instructions and protocol restrictions.
  2. Aged 18-80 years.
  3. The study subjects will be hypertensives with evidence of difficulty treating to target BP (daytime ABPM 135-170/85-105 mmHg) on 1 or more antihypertensive agents, with insufficient efficacy or intolerance of medications.
  4. For NITRATE LVH, echocardiographic evidence of LV hypertrophy (LV mass indexed to body surface area (BSA); males >115g/m2; females >95 g/m2).
  5. Patients will have been established on an antihypertensive treatment regime for at least 1 month by the time of participation in the study and will not require changes in pharmacological intervention for the duration of the trial.

Exclusion Criteria:

Unless specified, a subject will not be eligible for inclusion in this study if any of the following criteria apply:

  1. History of chronic viral hepatitis (including presence of hepatitis B surface antigen or hepatitis C antibody), or other chronic hepatic disorders.
  2. History of increased liver function tests (ALT, AST) due to acute or chronic liver conditions, 3x above the upper limit of normal or bilirubin 1.5x above the upper limit of normal at screening.
  3. Renal impairment with creatinine clearance (eGFR) of <50 ml/min at screening.
  4. Patients with diabetes mellitus, defined by previous history of diabetes or HbA1c >6.5% (>48 mmol/mol) at screening.
  5. Subjects with LDLc, >7.5 mmol/l. TG level >10mmol/l.
  6. History of heart failure defined as NYHA class II - IV or those with known LV dysfunction (EF<40%) regardless of symptomatic status
  7. History of malignancy within the past 5 years, other than non-melanoma skin cancer.
  8. Current life-threatening condition other than vascular disease (e.g. very severe chronic airways disease, HIV positive, life-threatening arrhythmias) that may prevent a subject from completing the study.
  9. Use of an investigational device or investigational drug within 30 days or 5 half-lives (whichever is longer) preceding the first dose of study medication.
  10. Subjects who will commence or who are likely to commence regular treatment with non-steroidal anti-inflammatory drugs (NSAIDs) (other than aspirin), from screening until study completion.
  11. Any non-stable dosing of ongoing medication regimens throughout the study trial.
  12. Drug abuse within the past 6 months.
  13. The subject has a three-month prior history of regular alcohol consumption exceeding an average weekly intake of > 28 units (or an average daily intake of greater than 3 units) for males, or an average weekly intake of > 21 units (or an average daily intake of greater than 2 units) for females. 1 unit is equivalent to a half-pint (284mL) of beer/lager; 25mL measure of spirits or 125mL of wine.
  14. Any other subject whom the Investigator deems unsuitable for the study (e.g. due to other medical reasons, laboratory abnormalities, expected study medication noncompliance, or subject's unwillingness to comply with all study-related study procedures).
  15. Subjects with rheumatoid arthritis, connective tissue disorders and other conditions known to be associated with chronic inflammation (e.g. Inflammatory Bowel Disease).
  16. Subjects with any acute infection, or recent systemic (oral or IV) antibiotics within 1 month of screening, or significant trauma (burns, fractures).
  17. Subjects who have donated more than 500 mL of blood within 56 days prior to the study medication administration.
  18. Self reported use of anti-microbial mouthwash or tongue scrapes.
  19. Concomitant xanthine oxidase inhibitors (such as allopurinol).
  20. Known history of significant claustrophobia, previous intolerance of CMR imaging or known (or suspected) incompatible metallic implant.
  21. Pregnancy.
  22. Allergy to gadolinium-based contrast agents used for CMR.
  23. Patients with known LVH caused by another established pathology diagnosed prior to or at screening e.g. severe aortic stenosis, hypertrophic cardiomyopathy, amyloidosis and Fabry's disease.

Exceptions to the exclusion criteria:

  • For criteria 18, patients can enter the trial if they discontinue the use of anti-microbial mouthwash for the duration of the clinical trial.
  • nCriteria 20 and 22 do not apply to participants who will not have a CMR scan in the NITRATE-CBP arm
Sex/Gender  ICMJE
Sexes Eligible for Study: All
Ages  ICMJE 18 Years to 80 Years   (Adult, Older Adult)
Accepts Healthy Volunteers  ICMJE No
Contacts  ICMJE
Contact: Amrita Ahulwalia, BSc PhD 02078825720 a.ahluwalia@qmul.ac.uk
Contact: Clement Lau, MBChB (Hons) 02078825720 c.lau@qmul.ac.uk
Listed Location Countries  ICMJE United Kingdom
Removed Location Countries  
 
Administrative Information
NCT Number  ICMJE NCT03088514
Other Study ID Numbers  ICMJE 10/H0703/98
Has Data Monitoring Committee Yes
U.S. FDA-regulated Product
Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
IPD Sharing Statement  ICMJE Not Provided
Responsible Party Amrita Ahluwalia, Queen Mary University of London
Study Sponsor  ICMJE Queen Mary University of London
Collaborators  ICMJE Barts & The London NHS Trust
Investigators  ICMJE
Principal Investigator: Amrita Ahulwalia, BSc PhD Queen Mary University of London
PRS Account Queen Mary University of London
Verification Date September 2020

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP