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Can Correction of Low Vitamin D Status in Infancy Program for a Leaner Body Composition?

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.
 
ClinicalTrials.gov Identifier: NCT02563015
Recruitment Status : Active, not recruiting
First Posted : September 29, 2015
Last Update Posted : February 5, 2020
Sponsor:
Information provided by (Responsible Party):
Hope Weiler, McGill University

Tracking Information
First Submitted Date  ICMJE September 28, 2015
First Posted Date  ICMJE September 29, 2015
Last Update Posted Date February 5, 2020
Actual Study Start Date  ICMJE March 7, 2016
Estimated Primary Completion Date February 20, 2022   (Final data collection date for primary outcome measure)
Current Primary Outcome Measures  ICMJE
 (submitted: September 28, 2015)
Lean mass [ Time Frame: 3 years ]
Whole body lean mass
Original Primary Outcome Measures  ICMJE Same as current
Change History
Current Secondary Outcome Measures  ICMJE
 (submitted: September 28, 2015)
25(OH)D [ Time Frame: 3 years ]
Concentration of serum 25(OH)D
Original Secondary Outcome Measures  ICMJE Same as current
Current Other Pre-specified Outcome Measures Not Provided
Original Other Pre-specified Outcome Measures Not Provided
 
Descriptive Information
Brief Title  ICMJE Can Correction of Low Vitamin D Status in Infancy Program for a Leaner Body Composition?
Official Title  ICMJE Novel Functional Outcomes of Vitamin D in Infancy; Can Correction of Low Vitamin D Status Program for a Leaner Body Composition Phenotype?
Brief Summary One in four infants are born with low amounts of vitamin D stored in their body. This study is designed to test whether improving vitamin D status quickly after birth helps infants to build muscle and to normalize growth. This is important since the investigators have noticed in previous work that infants with low vitamin D have higher body weight relative to body length later on and that those who develop very good stores quickly have a leaner body type. Therefore, in this study infants with low stores early after birth will be given either the regular amount of supplementation or a higher amount to more rapidly build up the vitamin stores in the body. Infants in both groups will be measured for muscle and fat mass at standardized ages during the first year of life and into the toddler years. The information will inform health care professionals and parents of the importance of establishing good vitamin D stores early in life. Vitamin D supplementation is a modifiable factor that is already recommended for all term born infants. Knowing how much is needed in infants born with low stores has not been tested in a controlled manner in Canada.
Detailed Description

Neonates from hospitals in the greater Montreal area will be studied. Neonates will be screened for low vitamin D status within 48 h of birth for 25(OH)D to enable rapid entry into study groups. Those with serum 25(OH)D <50 nmol/L will be randomized to 400 or 1000 IU/d until 1 y of age. Those with 25(OH)D >50 nmol/L will be provided standard of care (400 IU/d) and form a reference group.

Treatment period: Trial is 1 week to 12 months of age; follow-up to 3 years of age.

Frequency and duration of follow up: 1 week (baseline), 3, 6 and 12 months of age for the trial; then 2 and 3 years of age for the follow-ups.

At baseline (1 wk), 3, 6, 12, 24 and 36 mo of age, infants will be seen at our research unit for measurement of anthropometry, body composition and bone as well as blood sampling, skin pigmentation and surveys for diet, activity and demographic information.

Anthropometry - All measurements in infancy will be obtained nude and for 24 to 36 mo the child wearing standardized light clothing, dry diaper and no shoes. Weight will be taken using an electronic scale with a dynamic weighing program (Mettler-Toledo Inc., Switzerland). Length (0.1 cm) will be measured using an infantometer until 24 mo of age (O'Learly Length Boards, Ellard Instrumentation Ltd., US) and height at 36 mo will be measured using a stadiometer (Seca Medical Scales and Measuring Systems, US). Head circumference will be measured (0.1 cm) using a non-stretchable tape (Perspective Enterprises, US) to complete the anthropometric panel. Weight-for-age, height-for-age, and BMI-for-age Z-sores will be calculated using WHO software (WHO AnthroPlus, Switzerland). Mother's weight and height will be measured, while she is still breastfeeding, wearing standard clothing using a calibrated balance-beam scale (Detecto, Webb City, MO, USA) and a wall-mounted stadiometer (Seca model 226; new manufacturer Ayrton226 Hite-Rite Precision Mechanical Stadiometer) to calculate BMI.

Body Composition Measurements - Body composition will be assessed using a fan-beam DXA (APEX version 13.3:3, Hologic 4500A Discovery Series, Bedford, MA). Each infant will wear a light sleeper with no metal or plastic components and a diaper and be scanned using the infant whole body software; at 24 and 36 mo standardized light clothing will be worn and scans captured using whole body software. Whole body scans provide lean mass (g and % of weight), fat mass (g and %), BMC and BMD. Lumbar vertebra 1-4 and forearm BMC and BMD will be captured. Mother's body composition will be measured, while she is still breastfeeding, using BIA (Foot-to-foot tetrapolar Tanita TBF-310, Tanita Corp., Tokyo, Japan) as a rapid assessment.

Biochemistry Measurements - Capillary blood samples (0.5 ml) will be collected at screening; but venous sampling (1.0 ml: yields ~500-600 μl serum) used thereafter; samples will be centrifuged (2235 x g for 20 min at 4°C) to obtain serum (for biochemistry) and buffy coat (white blood cells for DNA) and stored frozen at -80°C until analysis. One 5 ml sample will be taken from parents at baseline (fasting) for measurement of serum 25(OH)D and buffy coat saved for future epigenetic work. For infant screening and maternal serum, total 25(OH)D will be measured using a dedicated auto-analyzer in the PI's laboratory (25 μl; 150 μl "dead volume" that is recovered, Liaison Diasorin Inc.); this assay will also be used for safety assessments at 3 and 6 mo, but is not to be used in analyzing the trial data outcomes as it does not capture all of the metabolites. Liquid chromatography tandem mass spectrometry (LC-MS/MS by Dr. Jones', Queen's Univ.) will be used to measure of 25(OH)D3, 3-epi- 25(OH)D3, and 24,25(OH)2D for all time-points from baseline to 36 mo. In addition, 1,25(OH)2D (100 μl serum) will be similarly measured using an adapted LC-MS/MS method. Both laboratories are certificated by the Vitamin D External Quality Assessment Scheme and will continue to participate in the National Institute of Standards and Technology quality assurance program. Blood-ionized calcium will be measured immediately using our portable unit (ABL80 FLEX Radiometer Medical A/S, Denmark) and compared to published standards (90). Remaining sample will be used for IGF-1 (20 μl) using Liaison (Diasorin Inc.); PTH (25 μl; Immutopics Inc CAT#60-3100) and IGFBP3 (20 μl; R&D Systems CAT#SGB300) will be measured by ELISA. Sample for IGFBP3 will be pre-treated with protease inhibitors prior to storage. Plasma total calcium and phosphate (150 μl) and urinary calcium and phosphate:creatinine will be measured in a spot sample collected at each visit during the trial; Beckman Coulter UniCel DxC600 autoanalyzer. We will reserve remaining sample for later measurement of C-telepeptide, propeptide of type 1 collagen (P1NP) as biomarkers related to bone.

Demographic, Dietary and Activity Surveys - At screening/baseline, parents will be asked to complete a demographic survey regarding their anthropometry, ethnicity and race, income and education using the same descriptors as defined by Statistics Canada. Infant dietary intake over the study period will be assessed using 3-day diet records completed by parents after each visit. While infants are breastfed, milk intake will be assessed by test-weighing of the infant before and after breast feeding for a 24-hour period using a portable electronic scale (Tanita Corporation Inc., US). This will provide nutrient intakes to help explain growth. Dietary intake from other foods is documented using household measurement items and recorded on the 3-day record. All nutrient analysis will be completed using the Nutritionist Pro software version 4.7.0 (Axxya Systems LLC, Stafford, TX) and the most recent Canadian Nutrient File database (Health Canada). At 2 and 3 y of age, the Habitual Activity Estimation Scale Questionnaire (HAES) will be completed by parents for a weekday (Tuesday, Wednesday, or Thursday) and weekend day (Saturday) over the past 2 weeks. Parents divide their child's day into 4 segments (wake-up to breakfast, breakfast to lunch, lunch to dinner, dinner to bedtime). For each time interval, the % time spent in each activity level is used to estimate overall level of physical activity. The 4 activity levels as established by the HAES questionnaire are: "inactive" (lying down, sleeping, resting), "somewhat inactive" (sitting, watching television, activities done mostly sitting down), "somewhat active" (walking, playing with toys), and "very active" (activities that make a child "breathe hard and sweat," like running and skipping).

Skin pigmentation and UVB exposure - Skin color (type) for the infant will be established by taking the average of three measurements at each site for constitutive pigmentation at the inner upper arm and facultative pigmentation (UVB exposure) at the forehead, mid-forearm and lower leg using a spectrophotometer (CM-700d/600d, Konica Minolta, USA). Individual typological angle (ITAo) will be calculated with the L* and b* values. Using constitutive pigmentation, infants will be classified into skin types (I-III: white; IV-VI: non-white) based on Fitzpatrick descriptions. Sun exposure, winter travel and use of sun block will also be surveyed. Sun exposure is expressed as a percentage of body surface area (BSA) exposed and then sun index calculated for each child by multiplying the percent BSA exposed by the time spent outside (min/d); this index does not include sun block.

Study Type  ICMJE Interventional
Study Phase  ICMJE Not Applicable
Study Design  ICMJE Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
Primary Purpose: Treatment
Condition  ICMJE
  • Healthy
  • Vitamin D Deficiency
Intervention  ICMJE
  • Dietary Supplement: cholecalciferol
    liquid drops
    Other Name: vitamin D3
  • Dietary Supplement: Reference 400
    liquid drops
    Other Name: vitaminD3
Study Arms  ICMJE
  • Experimental: Cholecalciferol 400
    400 IU orally per day
    Intervention: Dietary Supplement: cholecalciferol
  • Experimental: Cholecalciferol 1000
    1000 IU orally per day
    Intervention: Dietary Supplement: cholecalciferol
  • Active Comparator: Reference 400
    400 IU orally per day
    Intervention: Dietary Supplement: Reference 400
Publications * Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruitment Information
Recruitment Status  ICMJE Active, not recruiting
Actual Enrollment  ICMJE
 (submitted: February 4, 2020)
132
Original Estimated Enrollment  ICMJE
 (submitted: September 28, 2015)
222
Estimated Study Completion Date  ICMJE February 20, 2022
Estimated Primary Completion Date February 20, 2022   (Final data collection date for primary outcome measure)
Eligibility Criteria  ICMJE

Inclusion Criteria:

  • Term;
  • Healthy;
  • Appropriate weight for gestational age;
  • Infants born to mothers with otherwise healthy pregnancy and free of medications that impact vitamin D metabolism (except vitamin/mineral supplements) or fetal growth and intent to breastfeed to at least 3 months.

Exclusion Criteria:

  • Preterm;
  • Small for gestational age;
  • Maternal smoking in pregnancy, diabetes, preeclampsia, celiac disease, inflammatory bowel disease and medications that impact vitamin D/mineral metabolism.
Sex/Gender  ICMJE
Sexes Eligible for Study: All
Ages  ICMJE up to 1 Week   (Child)
Accepts Healthy Volunteers  ICMJE Yes
Contacts  ICMJE Contact information is only displayed when the study is recruiting subjects
Listed Location Countries  ICMJE Canada
Removed Location Countries  
 
Administrative Information
NCT Number  ICMJE NCT02563015
Other Study ID Numbers  ICMJE HW-15-01
Has Data Monitoring Committee No
U.S. FDA-regulated Product Not Provided
IPD Sharing Statement  ICMJE
Plan to Share IPD: Undecided
Responsible Party Hope Weiler, McGill University
Study Sponsor  ICMJE McGill University
Collaborators  ICMJE Not Provided
Investigators  ICMJE
Principal Investigator: Hope Weiler, PhD. McGill University
PRS Account McGill University
Verification Date February 2020

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP