Natural History and Genetics of Food Allergy and Related Conditions

• ClinicalTrials.gov Identifier: NCT02504853
• Recruitment Status: Recruiting
• First Posted: July 22, 2015
• Last Update Posted: May 24, 2023
• Sponsor: National Institute of Allergy and Infectious Diseases (NIAID)
• Information provided by (Responsible Party): National Institutes of Health Clinical Center (CC) ( National Institute of Allergy and Infectious Diseases (NIAID) )

Tracking Information

• First Submitted Date: July 21, 2015
• First Posted Date: July 22, 2015
• Last Update Posted Date: May 24, 2023
• Actual Study Start Date: July 29, 2015
• Estimated Primary Completion Date: June 15, 2025 (Final data collection date for primary outcome measure)

Current Primary Outcome Measures (submitted: March 7, 2019)

• Investigate the key genetic, cellular, immunologic, microbial, and biochemical pathways that lead to the development of food allergy [ Time Frame: 06/15/2025 ]
  Investigate the key genetic, cellular, immunologic, microbial, and biochemical pathways that lead to the development of food allergy
• Identify biomarkers that predict the clinical course and natural history of patients with food allergy [ Time Frame: 06/15/2025 ]
  Identify biomarkers that predict the clinical course and natural history of patients with food allergy

Original Primary Outcome Measures (submitted: July 21, 2015)

• Investigate the key genetic, cellular, immunologic, microbial, and biochemical pathways that lead to the development of food allergy [ Time Frame: 06/15/2025 ]
• Identify biomarkers that predict the clinical course and natural history of patients with food allergy [ Time Frame: 06/15/2025 ]

Current Secondary Outcome Measures (submitted: April 20, 2022)

• The prevalence of eosinophilic GI disease in patients who might be considered to be at high risk for these conditions, including those patients with atopic dermatitis and/or multiple food sensitivities/allergies [ Time Frame: 06/15/2025 ]
• In vitro testing of novel therapies for food allergy using cells and other biological specimens obtained from patients with food allergy [ Time Frame: 06/15/2025 ]
• Identification of nutritional deficiencies and their effect on the growth and overall health of patients with food allergy and related conditions [ Time Frame: 06/15/2025 ]

Original Secondary Outcome Measures (submitted: July 21, 2015)

• The prevalence of eosinophilic GI disease in patients who might be considered to be at high risk for these conditions, including those patients with atopic dermatitis and/or multiple food sensitivities/allergies [ Time Frame: 06/15/2026 ]
• Identification of nutritional deficiencies and their effect on the growth and overall health of patients with food allergy and related conditions [ Time Frame: 06/15/2026 ]
• In vitro testing of novel therapies for food allergy using cells and other biological specimens obtained from patients with food allergy [ Time Frame: 06/15/2026 ]

• Current Other Pre-specified Outcome Measures: Not Provided
• Original Other Pre-specified Outcome Measures: Not Provided

Descriptive Information

• Brief Title: Natural History and Genetics of Food Allergy and Related Conditions
• Official Title: Natural History and Genetics of Food Allergy and Related Conditions

Brief Summary

Background:

- About 15 million Americans have a food allergy. Because there are no cures or effective prevention or treatment for food allergies, researchers want to learn more about them.

Objective:

- To learn more about the causes and effects of food allergy and related conditions.

Eligibility:

• People ages 2 99 who have food allergy and/or a related genetic or other condition
• Their relatives
• Healthy relatives and volunteers

Design:

• Participants will have at least 3 visits over 1 2 years, and then once a year for up to 12 years. Each may last a day or longer.
• Participants will be screened with medical history, physical exam, and questionnaires.
• Participants may have the following:
• Blood tests
• Allergy skin prick tests: Drops of allergens are placed on the back or arm. The skin is scratched under each drop.
• Leukapheresis: blood is taken from a needle in one arm, passed through a machine, and returned through a needle in the other arm.
• X-rays
• Esophageal string test: One end of a string is taped to the cheek and the other end is packed into a capsule. When the capsule is swallowed, the string unwinds; it is left in for at least 1 hour.
• EGD and colonoscopy: Biopsies are taken from the gastrointestinal system.
• Tiny biopsies of skin
• Photographs of the body
• Collection of cells through:
• Swab of nose, inside of cheek, or skin
• Gentle skin scrape
• Tape stripping: piece of tape is put on the skin and pulled off.

Detailed Description

There are approximately 15 million Americans, including 6 million children, who have a potentially life-threatening food allergy. The prevalence of this disease has increased over the last three decades, in both the United States and other developed countries. There are no cures or effective prevention or treatment strategies for food allergy. Moreover, little is known about the factors that account for the rising prevalence and severity of these diseases in recent years. Both genetic and environmental factors likely contribute to the development of food allergy, but the complex interaction between these variables has frustrated efforts to elucidate pathogenesis and develop mechanism-targeted therapies. Children with food allergy are 2 to 4 times more likely to be diagnosed with asthma or other allergic conditions than children without food allergy, and food allergy may also be an important trigger for atopic dermatitis and eosinophilic esophagitis. The Laboratory of Allergic Diseases within the National Institute of Allergy and Infectious Diseases has a longstanding interest in the genetics and pathogenesis of allergic inflammatory disorders, and with the National Institutes of Health Clinical Center, it provides the ideal environment for the proposed translational studies. In this study, we will: (1) investigate the key genetic, cellular, immunologic, and biochemical pathways that lead to the development of food allergy, and (2) identify biomarkers that predict the clinical course and natural history of patients with food allergy.

Subjects eligible for enrollment in this study include children and adults with food allergy and patients with a known/suspected genetic or congenital disorder potentially associated with these phenotypes. Unaffected relatives (children and adults) of an enrolled subject and healthy volunteers (children and adults) will also be eligible for enrollment as controls. Most participants will be followed for 2 years, although participants with an identified genetic or congenital disorder and a subset of participants with food allergy may be followed until this study ends (up to 25 years).

Data obtained from analysis of blood, skin, saliva, stool, gastrointestinal biopsies, and other specimens will be used to explore the immunologic, biochemical, microbial, and genetic basis of food allergy. Results of research studies will be correlated with the scope and severity of their clinical phenotype, their response to treatment, and the natural history of their allergic disease(s).

• Study Type: Observational
• Study Design: Observational Model: Case-Control; Time Perspective: Prospective
• Target Follow-Up Duration: Not Provided
• Biospecimen: Not Provided
• Sampling Method: Non-Probability Sample
• Study Population: primary clinical

Condition

• Food Allergy
• Loeys-Dietz Syndrome
• Atopic Dermatitis
• Eosinophilic Esophagitis

• Intervention: Not Provided

Study Groups/Cohorts

• Affected Genetic
  Have a suspected genetic or congenital disorder potentially associated with food allergy or related condition, as determined by the principal investigator (PI) or associate investigators (AIs).
• Affected Non-Syndromic Food
  Individuals with a clinical history of immediate hypersensitivity reaction to foods and sensitized to food allergen(s) as evidenced by SPT or allergen-specific IgE testing.
• Allergic GI Disease
  Individuals with a diagnosis or clinical suspicion of eosinophilic esophagitis (EoE), as determined by the principal investigator (PI) or associate investigators (AIs).
• Unaffected Relative / Healthy Volunteer
  Unaffected relatives are relatives of affected; unaffected by food allergy or the genetic condition under study. Healthy volunteers are not related to affected and serve as controls.

Publications *

• Boyce JA, Assa'ad A, Burks AW, Jones SM, Sampson HA, Wood RA, Plaut M, Cooper SF, Fenton MJ, Arshad SH, Bahna SL, Beck LA, Byrd-Bredbenner C, Camargo CA Jr, Eichenfield L, Furuta GT, Hanifin JM, Jones C, Kraft M, Levy BD, Lieberman P, Luccioli S, McCall KM, Schneider LC, Simon RA, Simons FE, Teach SJ, Yawn BP, Schwaninger JM. Guidelines for the diagnosis and management of food allergy in the United States: summary of the NIAID-sponsored expert panel report. Nutr Res. 2011 Jan;31(1):61-75. doi: 10.1016/j.nutres.2011.01.001. No abstract available.
• Noval Rivas M, Burton OT, Wise P, Zhang YQ, Hobson SA, Garcia Lloret M, Chehoud C, Kuczynski J, DeSantis T, Warrington J, Hyde ER, Petrosino JF, Gerber GK, Bry L, Oettgen HC, Mazmanian SK, Chatila TA. A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis. J Allergy Clin Immunol. 2013 Jan;131(1):201-12. doi: 10.1016/j.jaci.2012.10.026. Epub 2012 Nov 30.
• Frischmeyer-Guerrerio PA, Guerrerio AL, Oswald G, Chichester K, Myers L, Halushka MK, Oliva-Hemker M, Wood RA, Dietz HC. TGFbeta receptor mutations impose a strong predisposition for human allergic disease. Sci Transl Med. 2013 Jul 24;5(195):195ra94. doi: 10.1126/scitranslmed.3006448.

Recruitment Information

• Recruitment Status: Recruiting
• Estimated Enrollment (submitted: November 26, 2022): 1800
• Original Estimated Enrollment (submitted: July 21, 2015): 1050
• Estimated Study Completion Date: June 15, 2025
• Estimated Primary Completion Date: June 15, 2025 (Final data collection date for primary outcome measure)

Eligibility Criteria

• GENERAL INCLUSION CRITERIA:

All participants must meet the following criteria:

1. Be 2 to 99 years-old at the time of enrollment for participants who will be seen at the NIH CC; be 0 (newborn) to 99 years-old at the time of enrollment for participants who will submit mail-in samples or participate in telehealth visits. Only viable neonates will be enrolled.
2. Willing to allow storage of blood, buccal swabs, saliva, nasal swabs, stool samples, and other clinically appropriate tissue specimens for future use in medical research
3. Required to have a primary care or other physician who will manage all health conditions related or unrelated to the study objectives

ADDITIONAL INCLUSION CRITERIA FOR AFFECTED PARTICIPANTS

In addition to the general criteria listed above, affected participants must meet 1 of the following criteria:

1. Have a clinical history of an immediate hypersensitivity reaction to food(s) and be sensitized to food allergen(s) (as evidenced by positive SPT or allergen-specific IgE testing)
2. Be sensitized (as evidenced by positive SPT or allergen-specific IgE testing) to food allergen(s) but have no overt clinical history of IgE-mediated symptoms when they eat that food(s)
3. Have a suspected genetic or congenital disorder potentially associated with food allergy or related condition, as determined by the principal investigator (PI) or associate investigators (AIs)
4. Have a diagnosis of eosinophilic esophagitis (EoE), or clinical suspicion for EoE as determined by the principal investigator (PI) or associate investigators (AIs)
5. Have a history of atopic dermatitis based on self-report or physician assessment.

ADDITIONAL INCLUSION CRITERIA FOR UNAFFECTED RELATIVES:

In addition to the general criteria listed above, unaffected relatives must meet the following criteria:

1. Be a relative of an affected participant
2. Be unaffected by food allergy (as determined by clinical history and/or allergy testing) or be unaffected by the genetic condition under study

ADDITIONAL INCLUSION CRITERIA FOR HEALTHY VOLUNTEERS

In addition to the general criteria listed above , healthy volunteers must meet the following criteria:

1. Be unrelated to an affected participant
2. Be unaffected by food allergy (as determined by clinical history and/or allergy testing) or be unaffected by the genetic condition under study

ADDITIONAL PROCEDURE- SPECIFIC INCLUSION CRITERIA:

The following additional inclusion criteria apply for participants undergoing leukapheresis:

1. Age greater than or equal to 18 years for affected participants, unaffected relatives, and healthy volunteers
2. Stable hematologic paramaters (definition: white blood cell count > 2,500/microL, hemoglobin > 10 g/dL, and platelet count > 75,000/microliter)

GENERAL EXCLUSION CRITERIA:

Participants will be excluded for any of the following:

• Presence of conditions that, in the judgment of the investigator or the referring physician, may put the participant at undue risk or make them unsuitable for participation in the study.
• Inability to participate for the duration of the study.
• The PI deems that participation in the study would not be expected to advance the study goals.

ADDITIONAL PROCEDURE- SPECIFIC EXCLUSION CRITERIA

Additional exclusion criteria apply for participants who are undergoing endoscopy with biopsies strictly for research purposes. Participants will be excluded from participating in this procedure if they meet any of the following criteria:

1. uncontrolled asthma or Grade 3 or higher by the American Society of Anesthesiologist s.Physical Status Classification System (http://www.asahq.org/resources/clinicalinformation/.asa-physical-status-classification-system)
2. history of adverse reaction to conscious sedation or general anesthesia required for endoscopy
3. hemoglobin < 11 g/dL
4. platelet count < 100,000 microL
5. PT INR >1.3 or PTT prolonged by > 3 seconds
6. pregnant or breastfeeding
7. viral screens positive for HIV or hepatitis B or C
8. severe unstable myocardial ischemia or cardiomyopathy
9. severe hypoxemia due to chronic pulmonary disease
10. recent abdominal surgery
11. anticoagulant therapy that cannot be interrupted

Additionally, unaffected relatives and healthy volunteers will be excluded from endoscopy if they meet any of the following criteria:

1. history of reflux disease within the last 3 years or use of acid-suppression medication (including over-the-counter medications such as Tums, Rolaids, Zantac, Prilosec OTC, or Prevacid OTC) within the last 2 months
2. chronic GI or immunologic disease
3. clinically indicated EGD or colonoscopy within the past 12 months
4. use of systemic or inhaled corticosteroids within the past 4 months
5. have a history of EGID including EoE
6. younger than 18 years of age

Sex/Gender

• Sexes Eligible for Study: All

• Ages: up to 99 Years (Child, Adult, Older Adult)
• Accepts Healthy Volunteers: Yes

Contacts

Contact: Ellen Zektser, R.N.; Not Listed; niaidfars@niaid.nih.gov

Contact: Pamela A Guerrerio, M.D.; (301) 402-9782; pamela.guerrerio@nih.gov

• Listed Location Countries: United States

Administrative Information

• NCT Number: NCT02504853
• Other Study ID Numbers: 150162; 15-I-0162
• Has Data Monitoring Committee: Not Provided

U.S. FDA-regulated Product

• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No

IPD Sharing Statement

• Plan to Share IPD: Yes
• Plan Description: .All collected IPD.
• Supporting Materials: Study Protocol
• Supporting Materials: Statistical Analysis Plan (SAP)
• Supporting Materials: Informed Consent Form (ICF)
• Supporting Materials: Analytic Code
• Time Frame: 6 months after publication.
• Access Criteria: Data that requires public reporting to be deposited in public databases, data required to be in publications, and data required for collaborations.

• Current Responsible Party: National Institutes of Health Clinical Center (CC) ( National Institute of Allergy and Infectious Diseases (NIAID) )
• Original Responsible Party: Same as current
• Current Study Sponsor: National Institute of Allergy and Infectious Diseases (NIAID)
• Original Study Sponsor: Same as current
• Collaborators: Not Provided

Investigators

• Principal Investigator: Pamela A Guerrerio, M.D.; National Institute of Allergy and Infectious Diseases (NIAID)

• PRS Account: National Institutes of Health Clinical Center (CC)
• Verification Date: May 22, 2023