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Enteral Versus Parenteral Glutamine Supplement

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00875797
Recruitment Status : Terminated (Termination due to interim analysis results, inclusion problems in small ICUs.)
First Posted : April 3, 2009
Results First Posted : January 16, 2014
Last Update Posted : January 16, 2014
Information provided by (Responsible Party):
Lidija Kompan, University Medical Centre Ljubljana

Tracking Information
First Submitted Date  ICMJE April 2, 2009
First Posted Date  ICMJE April 3, 2009
Results First Submitted Date  ICMJE June 27, 2013
Results First Posted Date  ICMJE January 16, 2014
Last Update Posted Date January 16, 2014
Study Start Date  ICMJE October 2004
Actual Primary Completion Date January 2009   (Final data collection date for primary outcome measure)
Current Primary Outcome Measures  ICMJE
 (submitted: December 1, 2013)
Intestinal Permeability - Lactulose-mannitol(L/M)Test [ Time Frame: 4 days after admission to intensive care unit ]
Measurement of intestinal permeability using lactulose-mannitol test (L/M test). Intestinal permeability to sugars is an accurate test for detecting intestinal damage. Intestinal permeability of the epithelium to very small sugar molecules such as lactulose/mannitol may give useful information regarding the overall condition of the digestive tract. Mannitol is absorbed transcellularly and lactulose has a paracellular route of absorption. Reduction in mannitol absorption shows reduced surface area and increased lactulose absorption indicates a leaky gut. Lactulose and mannitol are given orally and later determined from the collected urine with HPTLC (high performance thin layer chromatography). The L/M ratio, as a result of lactulose-mannitol tests, is then calculated regarding urine lactulose and mannitol concentrations. Thus, with the lactulose/mannitol test the intestinal permeability changes due to different reasons can be evaluated.
Original Primary Outcome Measures  ICMJE
 (submitted: April 2, 2009)
Measurement of intestinal permeability using lactulose-mannitol test [ Time Frame: 4 days after admission to intensive care unit ]
Change History Complete list of historical versions of study NCT00875797 on Archive Site
Current Secondary Outcome Measures  ICMJE
 (submitted: December 1, 2013)
  • Infection Rate at Participants in Both Groups [ Time Frame: participants were followed for the duration of ICU stay (average 3 weeks) ]
    Number of infections that occured at participants during study.
  • 6-month Survival [ Time Frame: 6 month ]
    Six month follow up
Original Secondary Outcome Measures  ICMJE
 (submitted: April 2, 2009)
Infection rate and survival [ Time Frame: 6 months survival ]
Current Other Pre-specified Outcome Measures Not Provided
Original Other Pre-specified Outcome Measures Not Provided
Descriptive Information
Brief Title  ICMJE Enteral Versus Parenteral Glutamine Supplement
Official Title  ICMJE Comparison of Effect of Enteral Versus Parenteral Glutamine Supplement on Intestinal Permeability and Outcome of Critically Ill Patients
Brief Summary

Glutamine is a major fuel for the intestinal tract and immune cells and therefore affects the intestinal permeability (IP) and infection rate at critically ill patients. The preferential route of glutamine supplementation at critically ill patients still remains open. Therefore the researchers will investigate IP, infection rate and treatment outcome at patients supplemented with either parenteral or enteral glutamine.

A prospective randomized single blind study is performed at mechanically ventilated. Patients were randomly assigned to either parenteral (group P) or enteral (group E) glutamine supplemented group. Early enteral feeding is started in both groups. Patients are/will be treated with glutamine for five days. IP will be measured using lactulose/mannitol test (L/M) on the fourth day.

Detailed Description

The patients who fulfilled the described criteria were randomly assigned to groups P or E using sequentially numbered containers for concealed randomization. Those in group P received the continuous infusion of parenteral glutamine dipeptide supplement (Dipeptiven 100ml, Fresenius Kabi) and were fed enterally with a standard commercial enteral polymeric diet without added glutamine (Ensure, Abbott Ross). The patients in group E received the enteral glutamine supplement as continuous administration of a standard commercial enteral diet supplemented with glutamine (Alitraq, Abbott Ross). The dose of the enteral glutamine, in a form of a free acid in this diet, depended on the volume of enteral food. Both groups of patients were treated with the glutamine supplement for five days. The other therapeutic procedures did not differ between the groups. All the patients were on continuous gastric feeding for 20 hours daily, starting with 20ml/hour. The enteral nutrition was started in 24 hours following admission. The gastric residual was measured three times daily, and when less than 250ml, the feeding volume was gradually increased up to 100ml/hour. If needed from day two onwards, the patients in all three groups received additional parenteral amino acid and glucose solutions to reach the goal 20kcal/kg/day and 0.15g nitrogen/kg/day.

Intestinal permeability (IP) was measured on day 4 using the lactulose/mannitol (L/M) test. For the study purpose, the patients were fasted 6 hours before the test. The test was performed with 5g of mannitol (M) and 10g of lactulose (L) mixed in 100ml of water. The mixture was given as a bolus via nasogastric tube. At the same time, 4ml of 20% chlorhexidine was added into an empty urine bag. The urine was collected in this bag for six hours. Then 5ml of urine was sampled from the bag and stored at -20˚C until analysis. Two hours after the test enteral feeding via nasogastric tube was started. Urinary L and M were determined simultaneously with thin-layer chromatography14, a new method in our laboratory for lactulose and mannitol determination. The method enabled determination of lactulose and mannitol in urine on the same amino HPTLC plate after densitometric quantification of lactulose by use of fluorescence mode, and mannitol by use of absorption mode after detection with AgNO3 reagent. The new method resulted in shorter analysis time, lower consumption of chemicals and HPTLC plates, increased sensitivity (lower limits of detection) and fewer problems with interfering compounds at determination of lactulose than the previously used two separate methods for determination of both analytes.14 The separation and quantification using this method are highly reproducible, yielding standard errors of less than 2.5% for retention times and less than 3.5% for quantitation.15,16 The investigators in the laboratory were blinded for the study groups. L/M index was calculated from urinary concentrations (c) of L and M using the following formula: L/M = c L / (c M x 2). L/M test was not performed at the beginning of the study because unstable trauma and septic patients were also included in the study, and at these patients, urine collection is difficult to perform.

Nosocomial infections were recorded during the entire ICU stay as recommended by the Centre for Disease Control in Atlanta.17,18 Nosocomial pneumonia was diagnosed when Hospital-Acquired Pneumonia Risk Index was 6 or more.19 The infections that were present upon admission or diagnosed within the first two days of ICU treatment were marked as acquired before ICU admission. All diagnosed infections were treated according to the results of microbiological tests and/or according to infection control guidelines. Acute inflammatory response was measured with C-reactive protein (CRP) levels. The blood samples for its determination were obtained at baseline and the end of the study.

Acute Physiology and Chronic Health Evaluation II (APACHE II) score was calculated upon admission. Each patient's ICU and hospital length of stay (LOS) and six month survival was recorded.

All participants were blinded to interventions. The ICU-staff was not blinded to group assignment, but they did not take part in the outcome assessment and on the other hand, the outcome assessors and laboratory personnel were blinded to group assignment.

Study Type  ICMJE Interventional
Study Phase  ICMJE Phase 4
Study Design  ICMJE Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double (Participant, Outcomes Assessor)
Primary Purpose: Treatment
Condition  ICMJE Critically Ill
Intervention  ICMJE
  • Dietary Supplement: parenteral glutamine
    Dipeptiven, Fresenius Kabi, Graz, Austria was given intravenously through central venous line in a dose up to 30 g per day
    Other Name: Dipeptiven, Fresenius Kabi, Graz, Austria
  • Dietary Supplement: enteral glutamine
    Alitraq, Abbott Laboratories, B.W. Zwolle, the Netherlands was given via nasogastric tube as continuous infusion of enteral diet, dose up to 30 g per day
    Other Name: Alitraq, Abbott Laboratories, B.W. Zwolle, the Netherlands
Study Arms  ICMJE
  • Active Comparator: parentral glutamine
    parenteral glutamine given in central venous line in dose up to 30 g par day
    Intervention: Dietary Supplement: parenteral glutamine
  • Experimental: entral glutamine
    enteral glutamine given through gastric tube in a dose up to 30 g per day
    Intervention: Dietary Supplement: enteral glutamine
Publications * Luo M, Bazargan N, Griffith DP, Estívariz CF, Leader LM, Easley KA, Daignault NM, Hao L, Meddings JB, Galloway JR, Blumberg JB, Jones DP, Ziegler TR. Metabolic effects of enteral versus parenteral alanyl-glutamine dipeptide administration in critically ill patients receiving enteral feeding: a pilot study. Clin Nutr. 2008 Apr;27(2):297-306. doi: 10.1016/j.clnu.2007.12.003. Epub 2008 Feb 7.

*   Includes publications given by the data provider as well as publications identified by Identifier (NCT Number) in Medline.
Recruitment Information
Recruitment Status  ICMJE Terminated
Actual Enrollment  ICMJE
 (submitted: December 1, 2013)
Original Actual Enrollment  ICMJE
 (submitted: April 2, 2009)
Actual Study Completion Date  ICMJE March 2009
Actual Primary Completion Date January 2009   (Final data collection date for primary outcome measure)
Eligibility Criteria  ICMJE

Inclusion Criteria:

  • mechanically ventilated critically-ill patients
  • older than 18 years
  • staying in intensive care unit for at least 4 days

Exclusion Criteria:

  • anuria
  • intestinal insufficiency (obstruction, discontinuation of intestine or severe ileus)
Sex/Gender  ICMJE
Sexes Eligible for Study: All
Ages  ICMJE 18 Years to 80 Years   (Adult, Older Adult)
Accepts Healthy Volunteers  ICMJE No
Contacts  ICMJE Contact information is only displayed when the study is recruiting subjects
Listed Location Countries  ICMJE Slovenia
Removed Location Countries  
Administrative Information
NCT Number  ICMJE NCT00875797
Other Study ID Numbers  ICMJE P4-0092: onko
Has Data Monitoring Committee Yes
U.S. FDA-regulated Product Not Provided
IPD Sharing Statement  ICMJE Not Provided
Responsible Party Lidija Kompan, University Medical Centre Ljubljana
Study Sponsor  ICMJE University Medical Centre Ljubljana
Collaborators  ICMJE Not Provided
Investigators  ICMJE
Principal Investigator: Jasna Uranjek, MD General Hospital Slovenj Gradec
PRS Account University Medical Centre Ljubljana
Verification Date December 2013

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP