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Can Amino Acid Supplementation Augment the Anabolic Response in Tendon After Exercise

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT04067479
Recruitment Status : Active, not recruiting
First Posted : August 26, 2019
Last Update Posted : August 26, 2019
Information provided by (Responsible Party):
Chad C. Carroll, Purdue University

Brief Summary:
The goal of this study is to determine if an oral provision of amino acids will enhance exercise-induced increases in markers of Achilles tendon collagen production.

Condition or disease Intervention/treatment Phase
Tendinopathy Dietary Supplement: Amino Acids Not Applicable

Detailed Description:

Tendon pain is a common complaint in older adults. The mechanisms contributing to the large number of tendon problems in older adults are not known. It has been shown that tendon cross-sectional area (CSA) is reduced with aging concurrent with a significant loss of tendon collagen. Exercise is used to reverse the loss of muscle mass with aging but surprisingly exercise interventions designed to reduce tendon problems in older people are relatively unexplored. In young adults, exercise stimulates tendon collagen synthesis and enhances the production of growth factors, which stimulate tendon collagen production. It is presumed that exercise-induced increase in collagen synthesis contributes to the increase in tendon stiffness and CSA noted in young adults after chronic resistance exercise (RE). In contrast, our preliminary work indicates that RE is not effective at reducing the effects of aging on tendon. The investigators have demonstrated that 12-weeks of RE does not lead to increases in tendon CSA or stiffness, even when skeletal muscle mass and strength are greatly enhanced. To prevent tendon injury, greater tendon CSA and stiffness aid in withstanding the force generated by trained skeletal muscle. Greater CSA and stiffness also optimize force transfer through tendon to maximize musculoskeletal function. Our findings indicate that RE is not an optimal intervention for reversing age-related changes in tendon properties. Novel interventions are clearly needed that will enhance collagen synthesis leading to an increase in tendon stiffness and CSA while also improving skeletal muscle mass and strength in older individuals. This study will evaluate the effectiveness of various methods for stimulating tendon collagen synthesis during a bout of acute exercise. Specifically, the investigators propose two approaches to this problem.

  1. An acute bout of cycling exercise will enhance the release of growth factors leading to a larger increase in tendon collagen synthesis.
  2. A bolus of amino acids given orally will increase the release of key stimulators of tendon collagen synthesis leading to enhanced collagen synthesis after acute exercise.

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Study Type : Interventional  (Clinical Trial)
Estimated Enrollment : 40 participants
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single (Participant)
Primary Purpose: Basic Science
Official Title: Can Amino Acid Supplementation Augment the Anabolic Response in Tendon After Exercise
Actual Study Start Date : May 9, 2017
Estimated Primary Completion Date : May 13, 2020
Estimated Study Completion Date : May 13, 2020

Resource links provided by the National Library of Medicine

Arm Intervention/treatment
Experimental: Young Dietary Supplement: Amino Acids
Oral bolus of essential amino acids

Experimental: Older Adults Dietary Supplement: Amino Acids
Oral bolus of essential amino acids

Primary Outcome Measures :
  1. Achilles peritendinous concentrations of pro-collagen [ Time Frame: 0-6 hours post consumption ]
    Peritendinous samples will be collected using microdialysis. Pro-collagen will be assessed using enzyme immunoassay kits

  2. Amino Acid Levels [ Time Frame: 0-6 hours post consumption ]
    Achilles peritendinous amino acid concentrations will be assess using high performance liquid chromatography.

Information from the National Library of Medicine

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Ages Eligible for Study:   21 Years to 80 Years   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • 21-35 years or 60-80 years old

Exclusion Criteria:

  • diabetes
  • BMI>35
  • acute illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, metabolic disorders, arthritis, or a history of neuromuscular problems.
  • chronic pain medication users
  • alcoholism
  • competitive athletes
  • pregnant women will be excluded
  • Individuals with specific allergies to amino acids

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT04067479

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United States, Indiana
Purdue University
West Lafayette, Indiana, United States, 47907
Sponsors and Collaborators
Purdue University
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Responsible Party: Chad C. Carroll, Assistant Professor, Purdue University Identifier: NCT04067479    
Other Study ID Numbers: 1704019133
First Posted: August 26, 2019    Key Record Dates
Last Update Posted: August 26, 2019
Last Verified: August 2019
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
Keywords provided by Chad C. Carroll, Purdue University:
amino acid
Additional relevant MeSH terms:
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Muscular Diseases
Musculoskeletal Diseases
Tendon Injuries
Wounds and Injuries