Group Culture May Affect Embryo Quality in Assisted Reproduction Technology
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|ClinicalTrials.gov Identifier: NCT04011319|
Recruitment Status : Recruiting
First Posted : July 8, 2019
Last Update Posted : July 8, 2019
|Condition or disease||Intervention/treatment||Phase|
|Embryo Quality||Behavioral: group culture||Not Applicable|
Providing optimal culture conditions for the embryos in ART is crucial, due to its high impact on embryo development and thus on the pregnancy outcome. Several strategies have been proposed in order to improve embryo culture conditions and clinical pregnancy rates. Currently, the establishment of autocrine and paracrine communications and embryo-to-embryo interactions should contribute to the detoxification of the culture medium, facilitating embryo development and improving the implantation rate. Many studies have provided evidence related to the impact of group culture condition on the success of the embryonic development in animals. But the advantage of group culture to improve the embryonic development and pregnancy outcomes of ART in human remains elusive. Therefore, the investigator aim to discuss the effectiveness of group culture in ART.
In this study, zygotes derived from sibling oocytes were randomized 1:1 to either the microwell group culture (experiment group) or conventional droplet culture (control group). In experiment group, 4 zygotes cultured in a new special microwell (120 μl droplet were divided into 4 areas) with a corral dish (EMBC-010, LifeGlobal), embryos can communicate with each other in the same culture environment. In control group, single embryo individually cultured in a droplet (60 μl). Embryos were cultured in specific desktop three-gas incubators (COOK) of 37°C, 6% CO2 (carbon dioxide) , 5% O2 and 89% N2, and scored according to the criteria described in the ESHRE-ALPHA consensus. Consequently, exploitable embryos rate, good embryo quality, blastocyst formation rate were recorded. Other relevant parameters such as clinical pregnancy, live-birth or miscarriage were also recorded to evaluate the outcomes. Proper culture conditions can result in superior embryo viability, this research intends to discuss whether group culture can improve the embryo quality and clinical outcomes in ART.
|Study Type :||Interventional (Clinical Trial)|
|Estimated Enrollment :||2000 participants|
|Intervention Model:||Parallel Assignment|
|Official Title:||Group Culture May Affect Human Embryo Quality in Assisted Reproduction Technology|
|Actual Study Start Date :||April 1, 2019|
|Estimated Primary Completion Date :||March 2020|
|Estimated Study Completion Date :||March 2020|
No Intervention: convention culture
Each droplet separate culturing an individual embryo.
Experimental: group culture
Embryos were cultured in the same droplet.
Behavioral: group culture
Four embryos were cultured in the same droplet by a special dish.
- Efficacy of group culture on embryo potential [ Time Frame: 1 year ]Embryo potential was evaluated by the clinical pregnancy rate
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT04011319
|Contact: Ming Wangfirstname.lastname@example.org|
|Xi'an, Shaanxi, China, 710038|
|Contact: Ming Wang|
|Study Director:||Bo Li||Tang-Du Hospital|