Mitochondrial DNA in Fresh Versus Frozen Embryo Culture Media of PCOS Women Undergoing In Vitro Fertilization
|ClinicalTrials.gov Identifier: NCT03820167|
Recruitment Status : Completed
First Posted : January 29, 2019
Last Update Posted : February 26, 2020
|Condition or disease|
|Polycystic Ovary Syndrome Assisted Reproduction|
|Study Type :||Observational|
|Actual Enrollment :||90 participants|
|Official Title:||Mitochondrial DNA in Fresh Versus Frozen Embryo Culture Media of Polycystic Ovarian Syndrome Patients Undergoing In Vitro Fertilization|
|Actual Study Start Date :||January 30, 2019|
|Actual Primary Completion Date :||December 25, 2019|
|Actual Study Completion Date :||January 14, 2020|
Morphologically good embryos will be cultured in a culture dish and the supernatants will be collected freshly from culture system at day 3 and stored at -20 ̊ c until being tested.
Quantification of mtDNA in fresh and frozen culture media using qPCR technique.
Morphologically good embryos scheduled for freezing will be cryopreserved for less than one year and thawed, the supernatant will be collected and stored at -20 ̊ c until being tested. Quantification of mtDNA in fresh and frozen culture media using qPCR technique.
- clinical pregnancy rate [ Time Frame: 4 weeks after embryo transfer ]detection of intrauterine gestational sac by transvaginal ultrasound
Biospecimen Retention: Samples With DNA
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03820167
|Ganna IVF center|
|Giza, Cairo, Egypt, 12111|
|Principal Investigator:||Ahmed Maged, MD||Professor|