Impact of Periodontal Treatment on the RANKL/OPG Ratio in Crevicular Fluid
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|ClinicalTrials.gov Identifier: NCT03787875|
Recruitment Status : Completed
First Posted : December 25, 2018
Last Update Posted : December 25, 2018
Objectives: Alveolar resorption is one of the most important events in periodontitis. Osteoclast activity is regulated by the ratio between receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG). The aim of this study was to evaluate changes in the RANKL/OPG ratio in crevicular fluid after periodontal treatment.
Material and methods: A total of 15 patients with periodontitis were included in the study group. Samples were collected from an area with active periodontitis and a healthy area. The RANKL and OPG levels were measured before and after periodontal scaling and root planing (SRP) treatment. The study group was compared to the control group, which included 10 patients without periodontitis.
|Condition or disease||Intervention/treatment|
|Periodontal Diseases Biomarkers Bone Resorption||Procedure: periodontal treatment|
Show Detailed Description
|Study Type :||Observational|
|Actual Enrollment :||40 participants|
|Official Title:||Impact of Periodontal Treatment on the RANKL/OPG Ratio in Crevicular Fluid|
|Actual Study Start Date :||February 2, 2016|
|Actual Primary Completion Date :||June 5, 2016|
|Actual Study Completion Date :||November 6, 2016|
The control group included 10 periodontally healthy subjects without signs or symptoms of periodontal disease.
The study group included 15 subjects diagnosed with mild or advanced chronic periodontitis. Each periodontal patient had one non-affected single-rooted tooth (healthy site) and another single-rooted tooth with periodontitis (periodontitis site) with the following features:
Healthy site: a single-rooted tooth with probing depths below or equal to 3 mm without recession and without bleeding on probing.
Periodontitis site: another single-rooted tooth from the same patient with clinical attachment loss equal to or greater than 6 mm and bleeding upon probing.
Procedure: periodontal treatment
In the study group, periodontal scaling and root planning treatment of the 4 quadrants was performed for two consecutive weeks without the use of antiseptics or antibiotics, and the patients were instructed in oral hygiene, including both brushing techniques and proximal hygiene.
- Levels of RANKL in crevicular fluid [ Time Frame: 49 days ]
receptor activator of NF-κB ligand.RANKL is a protein that determines the activation of osteoclasts by binding to the RANK membrane receptor that preosteoclates possess.
The ranks wi be measured in μg / μl.
- Levels of OPG in crevicular fluid [ Time Frame: 49 days ]
osteoprotegerin.Osteoprogesterian is a soluble protein that acts as a decoy blocked RANK receptor and thus initiating osteoclastogenesis.
The opg will be measured in μg / μl.
- probing depth [ Time Frame: 49 days ]Probing depth, is the distance from the free gingival margin to the bottom of the periodontal pocket. This parameter will be measured in mm.
- clinical attachment level [ Time Frame: 49 days ]Is the distance from the free cementoenamel junction to the bottom of the periodontal pocket. This parameter will be measured in mm.
- bleeding upon probing [ Time Frame: 49 days ]Percentage of points with bleeding on probing in relation to the total number of points probed.