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Examining the Impact of Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males

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ClinicalTrials.gov Identifier: NCT03717948
Recruitment Status : Completed
First Posted : October 24, 2018
Last Update Posted : July 15, 2019
Sponsor:
Information provided by (Responsible Party):
Brendon Gurd, Queen's University

Brief Summary:
This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.

Condition or disease Intervention/treatment Phase
Mitochondrial Biogenesis Other: BFR Exercise Other: CTL Exercise Not Applicable

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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 13 participants
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double (Investigator, Outcomes Assessor)
Masking Description: Allocation concealment, assessor blinding
Primary Purpose: Basic Science
Official Title: Examining the Impact of Acute Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males: A Randomized Cross-over Trial
Actual Study Start Date : October 1, 2018
Actual Primary Completion Date : January 28, 2019
Actual Study Completion Date : January 28, 2019

Resource links provided by the National Library of Medicine


Arm Intervention/treatment
Experimental: CTL - BFR
CTL Exercise then BFR Exercise
Other: BFR Exercise
Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR).

Other: CTL Exercise
Participants will exercise on a recumbent bike placed on flat ground (CTL).

Experimental: BFR - CTL
BFR Exercise then CTL Exercise
Other: BFR Exercise
Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR).

Other: CTL Exercise
Participants will exercise on a recumbent bike placed on flat ground (CTL).




Primary Outcome Measures :
  1. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) [ Time Frame: 15 minutes before exercise and 3 hours post-exercise for both exercise sessions ]
    Fold-change in PGC-1α mRNA expression


Secondary Outcome Measures :
  1. Catecholamine concentrations [ Time Frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions ]
    Catecholamine concentrations in venous blood

  2. Total and phosphorylated p38 mitogen-activated protein kinase (MAPK) [ Time Frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions ]
    Fold change in p38 MAPK phosphorylation

  3. Total and phosphorylated acetyl coenzyme A carboxylase (ACC) [ Time Frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions ]
    Fold change in ACC phosphorylation

  4. Total and phosphorylated protein kinase A (PKA) [ Time Frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions ]
    Fold change in PKA phosphorylation

  5. Acetylated p53 [ Time Frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions ]
    Fold change in acetylated p53

  6. Blood lactate [ Time Frame: During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions ]
    Average [lactate] between conditions

  7. Heart rate (HR) [ Time Frame: During both exercise sessions ]
    Average HR between conditions

  8. Revolutions per minute (RPM) [ Time Frame: During both exercise sessions ]
    Average RPM between conditions

  9. Ratings of perceived exertion (RPE) [ Time Frame: During both exercise sessions ]
    Average RPE between conditions

  10. Oxygenated and deoxygenated hemoglobin [ Time Frame: During both exercise sessions and up to 10 minutes after (for cuff occlusion) ]
    Average oxygenated and deoxygenated hemoglobin as a percentage of same day max values



Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 30 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • 3 hours of physical activity per week
  • No previous cycling training
  • No concurrent involvement in another training program
  • Body mass index < 30 kg/m2

Exclusion Criteria:

  • Presence of cardiovascular disease
  • Presence of metabolic disease
  • Taking regular oral medication
  • Current smoker

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03717948


Locations
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Canada, Ontario
Queen's University
Kingston, Ontario, Canada, K7L3N6
Sponsors and Collaborators
Queen's University
Investigators
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Principal Investigator: Brendon J Gurd, PhD Queen's University
  Study Documents (Full-Text)

Documents provided by Brendon Gurd, Queen's University:
Statistical Analysis Plan  [PDF] June 24, 2019


Publications automatically indexed to this study by ClinicalTrials.gov Identifier (NCT Number):
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Responsible Party: Brendon Gurd, Associate Professor, Queen's University
ClinicalTrials.gov Identifier: NCT03717948    
Other Study ID Numbers: BFRNP
402635 ( Other Grant/Funding Number: NSERC )
First Posted: October 24, 2018    Key Record Dates
Last Update Posted: July 15, 2019
Last Verified: July 2019

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
Keywords provided by Brendon Gurd, Queen's University:
Acute Exercise
Mitochondria
Skeletal Muscle
PGC1α
Mitochondrial Biogenesis
Intracellular Signalling