Differential Expression of CD200 in B-chronic Lymphoproliferative Disorders by Multicolour Flow Cytometry
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|ClinicalTrials.gov Identifier: NCT03712488|
Recruitment Status : Not yet recruiting
First Posted : October 19, 2018
Last Update Posted : October 19, 2018
The investigator's goals in this study are to assess :
- Differential expression of CD200 by using flow cytometric immune-phenotyping in broad range of patients with B-chronic lymphoproliferative disorders (B-CLPD)
- Role of CD200 in diagnosis , classification and potential value in differential diagnosis
- CD200 expression level at different anatomic sites
|Condition or disease||Intervention/treatment|
B-Chronic lymphoproliferative disorders (B-CLPDs) are heterogeneous group of disorders with variable clinical presentations and outcomes. They are classified into : chronic lymphocytic leukaemia (CLL), B‐cell prolymphocytic leukaemia (B‐PLL), hairy cell leukaemia (HCL), and mantle cell lymphoma (MCL), marginal zone lymphoma (MZL), follicular lymphoma (FL) and lymphoplasmacytic lymphoma/WaldenstrÖm macroglobulinaemia (LPL/WM) in leukaemia phase.
Characterization of CLPDs by immunophenotyping (IPT) has become an important and widely used method in hematology. Immunophenotyping is indispensable for the diagnosis of B-CLPDs through recognition of restricted light chain expression and characteristic phenotypes of separate entities.
Immunophenotypic characterization of lymphoid neoplasms is important for diagnosis, sub-classification, and staging and can also play a role in monitoring minimal residual disease. However, the differential diagnosis may be difficult to resolve in some cases, for example, between B-cell neoplasms with full or partial CD5 expression.
CD200 has recently been identified as a potentially useful antigen for flow cytometric immunophenotyping of lymphoid neoplasms, particularly those of the B lineage.
CD200 belongs to the immunoglobulin superfamily and is composed of a light chain-like structure with two extracellular variable- and constant-like domains followed by a transmembrane segment and a cytoplasmic tail.5 CD200 is expressed by various cell types, including B cells, a subset of T cells (including activated T cells), thymocytes, endothelial cells, and neurons .
CD200 generates an immunosuppressive signal by binding to its cognate receptor, CD200 receptor 1 (CD200R1) , which is expressed specifically in granulocytes and monocytes and in a subset of T cells. CD200 appears to play a role in the regulation of antitumor activity and these findings are the basis for ongoing clinical trials using anti-CD200 therapy for chronic lymphocytic leukemia (CLL)
|Study Type :||Observational|
|Estimated Enrollment :||50 participants|
|Official Title:||Differential Expression of CD200 in B-chronic Lymphoproliferative Disorders by Multicolour Flow Cytometry|
|Estimated Study Start Date :||December 15, 2018|
|Estimated Primary Completion Date :||August 30, 2020|
|Estimated Study Completion Date :||April 1, 2021|
- Device: flowcytometry
four colour flowcytometry
- Differential expression of CD200 by using flow cytometric immune-phenotyping in B-chronic lymphoproliferative disorders (B-CLPD) [ Time Frame: baseline ]Analysis of expression of CD200 in B-CLPDs for differential diagnosis of subtypes of B-CLPDs
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03712488
|Contact: menna M atef, email@example.com|
|Study Director:||douaa M Sayed, professor||South Egypt Cancer Institute|