TNF-α/HIF-1α/VEGF Pathway in Periodontal Diseases
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|ClinicalTrials.gov Identifier: NCT03558906|
Recruitment Status : Completed
First Posted : June 15, 2018
Last Update Posted : June 15, 2018
Aydin Adnan Menderes University
Celal Bayar University
Information provided by (Responsible Party):
Beral Afacan, Aydin Adnan Menderes University
This study aimed to investigate gingival crevicular fluid (GCF) and salivary hypoxia inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-α) levels in different periodontal diseases. A total of 87 individuals, 20 patients with generalized aggressive periodontitis, 20 with chronic periodontitis, 26 with gingivitis and 21 periodontally healthy individuals were included. Whole-mouth and site-specific clinical periodontal parameters including probing depth, clinical attachment level, bleeding on probing, gingival index and plaque index were recorded. GCF and salivary HIF-1α, VEGF and TNF-α levels were measured by enzyme-linked immunosorbent assay. Statistical analysis was performed by using non-parametric tests.
|Condition or disease|
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|Study Type :||Observational|
|Actual Enrollment :||87 participants|
|Official Title:||Gingival Crevicular Fluid and Salivary HIF-1α, VEGF and TNF-α Levels in Different Periodontal Diseases|
|Actual Study Start Date :||March 1, 2016|
|Actual Primary Completion Date :||March 2, 2016|
|Actual Study Completion Date :||December 26, 2016|
These individuals had minimum PD ≥6 mm and CAL ≥5 mm on eight or more teeth; at least three of these were other than central incisors or first molars. Radiographic alveolar bone loss was ≥30% of root length affecting at least three permanent teeth other than first molars and incisors. The severe destruction pattern was not commensurate with amount of plaque accumulation.
These individuals had at least four non-adjacent teeth with sites with PD ≥6 mm and CAL ≥5 mm. They had also ≥50% alveolar bone loss in at least two quadrants that was commensurate with amount of plaque accumulation. BOP was >50% in the whole mouth.
Gingivitis patients exhibited no sites with CAL >2 mm and no detectable alveolar bone loss in the radiography. BOP was >50% in the whole mouth.
Periodontally healthy volunteers exhibited no sites with PD >3 mm and CAL >2 mm as well as no radiographic evidence of alveolar bone loss. BOP was <15% in the whole mouth.
Primary Outcome Measures :
- Gingival crevicular fluid HIF-1α levels [ Time Frame: A day after clinical periodontal examination ]pg
Secondary Outcome Measures :
- Salivary HIF-1α levels [ Time Frame: A day after clinical periodontal examination ]pg/ml
- Gingival crevicular fluid VEGF levels [ Time Frame: A day after clinical periodontal examination ]ng
- Salivary VEGF levels [ Time Frame: A day after clinical periodontal examination ]ng/l
Biospecimen Retention: Samples Without DNA
Gingival crevicular fluid, saliva
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