Modified T Cells, Chemotherapy, and Aldesleukin With or Without LV305 and CMB305 in Treating Participants With Advanced or Recurrent Sarcoma
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ClinicalTrials.gov Identifier: NCT03450122 |
Recruitment Status :
Recruiting
First Posted : March 1, 2018
Last Update Posted : March 23, 2020
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Condition or disease | Intervention/treatment | Phase |
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HLA-A*0201 Positive Cells Present NY-ESO-1 Positive Tumor Cells Present Recurrent Myxoid Liposarcoma Recurrent Synovial Sarcoma | Biological: Aldesleukin Biological: Autologous NY-ESO-1-specific CD8-positive T Lymphocytes Drug: Cyclophosphamide Biological: Dendritic Cell-targeting Lentiviral Vector ID-LV305 Drug: Immunotherapeutic Combination Product CMB305 | Phase 1 |
PRIMARY OBJECTIVES:
I. Evaluate the safety of adoptively transferred CD8 T cells targeting NY-ESO-1 positive (+) tumors given alone and in combination with antigen-specific vaccination.
II. Evaluate the functional and numeric in vivo persistence of NY-ESO-1-specific CD8 T-cells given alone and in combination with antigen-specific vaccination.
SECONDARY OBJECTIVES:
I. Evaluate the anti-tumor efficacy achieved following adoptive transfer of NY-ESO-specific CD8 T cells in combination with LV305 alone and with G305 vaccine in patients with advanced synovial and mixed round cell liposarcoma.
II. Evaluate the influence of antigen-specific vaccination on the induction of both CD8 and CD4 T cells to NY-ESO-1 and non-targeted tumor-associated antigens (antigen-spreading) and the correlation of these responses with clinical outcome.
OUTLINE: Participants are assigned to 1 of 3 groups.
COHORT 0: Participants receive cyclophosphamide intravenously (IV) over 30-60 minutes on day -2 and autologous NY-ESO-1-specific CD8-positive T lymphocytes IV over 60 minutes on day 0. Then, 6 hours later and twice a day for 14 days, receive aldesleukin subcutaneously (SC) in the absence of disease progression or unacceptable toxicity.
COHORT 1: Participants receive cyclophosphamide, autologous NY-ESO-1-specific CD8-positive T lymphocytes, and aldesleukin as in Cohort 0. Participants also receive dendritic cell-targeting lentiviral vector ID-LV305 intradermally (ID) on days 1, 22, 43, and 64 in the absence of disease progression or unacceptable toxicity.
COHORT 2: Participants receive cyclophosphamide, autologous NY-ESO-1-specific CD8-positive T lymphocytes and aldesleukin as in Cohort 0. Participants also receive dendritic cell-targeting lentiviral vector ID-LV305 injection on days 1, 14, 43, and 70, and immunotherapeutic combination product CMB305 intramuscularly (IM) on days 29, 57, and 85 in the absence of disease progression or unacceptable toxicity.
After conclusion of study treatment, participants are followed up every 4 weeks for 168 days, then every 3 months for 24 months.
Study Type : | Interventional (Clinical Trial) |
Estimated Enrollment : | 18 participants |
Allocation: | Non-Randomized |
Intervention Model: | Parallel Assignment |
Masking: | None (Open Label) |
Primary Purpose: | Treatment |
Official Title: | Phase I Study of Cellular Adoptive Immunotherapy Using Autologous CD8+ NYESO-1-Specific T Cells and the NY-ESO-1 Immunostimulatory Agents LV305 or CMB305 for Patients With Sarcoma |
Actual Study Start Date : | September 13, 2018 |
Estimated Primary Completion Date : | June 30, 2021 |
Estimated Study Completion Date : | June 30, 2021 |

Arm | Intervention/treatment |
---|---|
Experimental: Cohort 0 (cyclophosphamide, T cells, aldesleukin)
Participants receive cyclophosphamide IV over 30-60 minutes on day -2 and autologous NY-ESO-1-specific CD8-positive T lymphocytes IV over 60 minutes on day 0. Then, 6 hours later and twice a day for 14 days, receive aldesleukin SC in the absence of disease progression or unacceptable toxicity.
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Biological: Aldesleukin
Given SC
Other Names:
Biological: Autologous NY-ESO-1-specific CD8-positive T Lymphocytes Given IV Drug: Cyclophosphamide Given IV
Other Names:
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Experimental: Cohort 1 (cyclophosphamide, T cells, aldesleukin, LV305)
Participants receive cyclophosphamide, autologous NY-ESO-1-specific CD8-positive T lymphocytes, and aldesleukin as in Cohort 0. Participants also receive dendritic cell-targeting lentiviral vector ID-LV305 ID on days 1, 22, 43, and 64 in the absence of disease progression or unacceptable toxicity.
|
Biological: Aldesleukin
Given SC
Other Names:
Biological: Autologous NY-ESO-1-specific CD8-positive T Lymphocytes Given IV Drug: Cyclophosphamide Given IV
Other Names:
Biological: Dendritic Cell-targeting Lentiviral Vector ID-LV305 Given ID
Other Names:
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Experimental: Cohort 2(cyclophosphamide,T cells, aldesleukin, LV305, CMB305)
Participants receive cyclophosphamide, autologous NY-ESO-1-specific CD8-positive T lymphocytes and aldesleukin as in Cohort 0. Participants also receive dendritic cell-targeting lentiviral vector ID-LV305 injection on days 1, 14, 43, and 70, and immunotherapeutic combination product CMB305 IM on days 29, 57, and 85 in the absence of disease progression or unacceptable toxicity.
|
Biological: Aldesleukin
Given SC
Other Names:
Biological: Autologous NY-ESO-1-specific CD8-positive T Lymphocytes Given IV Drug: Cyclophosphamide Given IV
Other Names:
Biological: Dendritic Cell-targeting Lentiviral Vector ID-LV305 Given ID
Other Names:
Drug: Immunotherapeutic Combination Product CMB305 Given IM
Other Names:
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- Duration of in vivo persistence of transferred T cells [ Time Frame: Up to 168 days ]Will be assessed alone or in combination with dendritic cell-targeting lentiviral vector ID-LV305 (LV305) or in combination with CMB305.
- The nature, frequency and severity of adverse events [ Time Frame: Up to 168 days ]n subjects receiving NY-ESO-1-specific T cells alone, T cells with LV305 and T cells with CMB305
- The Laboratory abnormalities [ Time Frame: Up to 168 days ]Will be assessed in subjects receiving NY-ESO-1-specific T cells alone, T cells with LV305 and T cells with CMB305
- Tumor response per Response Evaluation Criteria in Solid Tumors (RECIST) v1.1 and RECIST-based immune-related response (irRC) data [ Time Frame: Up to 168 days ]CT/MRI will be evaluated per RECIST v1.1 and RECIST- based irRC
- Persistence of cellular immune response [ Time Frame: Up to 168 days ]Research blood draws at specific intervals will be collected and analyze for these outcomes
- Differentiation phenotype [ Time Frame: Up to 168 days ]Research blood draws at specific intervals will be collected and analyze for these outcomes
- Antigen-spreading [ Time Frame: Up to 168 days ]Research blood draws at specific intervals will be collected and analyze for these outcomes

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Ages Eligible for Study: | 18 Years and older (Adult, Older Adult) |
Sexes Eligible for Study: | All |
Accepts Healthy Volunteers: | No |
Inclusion Criteria:
- Histopathologic documentation of synovial sarcoma or myxoid liposarcoma with the diagnosis of advanced or recurrent disease who have received prior standard chemotherapy. Patients with other sarcoma subtypes if proven to be NY-ESO-1 positive and meeting all other eligibility criteria listed below will also be included.
- Tumor expression of NY-ESO-1 (2+ staining or > 25%) by immunohistochemistry (IHC).
- Expression of HLA-A*0201.
- Eastern Cooperative Oncology Group (ECOG)/ Zubrod performance status of '0-1'
- Life expectancy > 6 months.
- Electrocardiography (ECG) without evidence of clinically significant arrhythmia or ischemia.
- Women of childbearing potential (WOCBP) must be using at least one highly effective or two effective accepted methods of contraception to avoid conception throughout the study in such a manner that the risk of pregnancy is minimized. Suggested precautions should be used to minimize the risk or pregnancy for at least 1 month before start of therapy, and while women are on study for up to 3 months after T cell infusion and/or at least 3 months after the study agents LV305 or CMB305 are stopped. WOCBP include any female who has experienced menarche and who has not undergone successful surgical sterilization (hysterectomy, bilateral tubal ligation or bilateral oophorectomy) or is not postmenopausal.
- Men must be willing and able to use an acceptable method of birth control such as latex condom during the dosing period and for at least 3 months after completion of the study agent administration (T cell infusion and/or LV305 or CMB305) if their sexual partners are WOCBP.
- Willing and able to give informed consent.
- (Prior to treatment) Note: evaluate at least 1 week before T cell infusion. a. Adequate venous access - consider peripherally inserted central catheter (PICC) or central line. b. ECOG/Zubrod performance status of '0-1. c. Bi-dimensionally measurable disease by palpation on clinical exam, or radiographic imaging (X-ray, computed tomography [CT scan]). d. At least 4 weeks must have elapsed since the last chemotherapy, immunotherapy, radiotherapy or major surgery. At least 6 weeks for nitrosoureas, mitomycin C and liposomal doxorubicin. e. Toxicity related to prior therapy must either have returned to =< grade 1, baseline, or been deemed irreversible. f. Persons of reproductive potential must agree to use and utilize an adequate method of contraception throughout treatment and for at least 3 months after study drug is stopped. g. Willing and able to give informed consent.
Exclusion Criteria:
- Patients with active infections or oral temperature > 38.2 Celsius (C) within 72 hours of leukapheresis. The procedure may be deferred.
- Investigational therapy within 3 weeks.
- Prior administration of other NY-ESO-1 targeting immunotherapeutics.
- Significant immunosuppression from concurrent, recent (=< 4 weeks ago) or anticipated treatment with systemic corticosteroids at any dose, or other immunosuppressive medications such as methotrexate, cyclosporine, azathioprine (antihistamines, non-steroidal anti-inflammatory drugs and aspirin permitted) or conditions such as common variable hypogammaglobulinemia or exposures such as large field radiotherapy.
- Cancer therapies, including chemotherapy, radiation, biologic, or kinase inhibitors, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) within 3 weeks prior.
- Psychiatric, other medical illness or other condition that in the opinion of the principal investigator (PI) prevents compliance with study procedures or ability to provide valid informed consent.
- Significant autoimmune disease with the exception of alopecia, vitiligo, hypothyroidism or other conditions that have never been clinically active or were transient and have completely resolved and require no ongoing therapy.
- Myocardial infarction within 6 months of study initiation, active cardiac ischemia or New York Heart Association (NYHA) grade III or IV heart failure.
- Peripheral blood leukocyte count (white blood cells [WBC]) < 3000/mm^3.
- Absolute neutrophil count =< 1500/mm^3.
- Platelets < 75000/mm^3.
- Hemoglobin < 10 gm/dL.
- Alanine aminotransferase (ALT), and aspartate aminotransferase (AST) > 2.5 x upper limit of normal (ULN).
- Total serum bilirubin > 1.5 x ULN (patients with Gilbert's disease may be included if their total bilirubin is =< 3.0 mg/dL).
- Creatinine > 1.5 x ULN. If higher check 24hr clearance, if < 50 ml/min then patient will be excluded.
- INR (prothrombin time ratio) or partial thromboplastin time (PTT) > 1.5 x ULN (Please note: patients with hematopoietic cell transplantation (Hct) < 30%, WBC < 2500/mm/^3 and platelets < 50,000/mm^3 immediately prior to leukapheresis. The procedure may be deferred.)
- History of other cancer within 3 years (except non-melanoma cutaneous malignancies and cervical carcinoma in situ).
- Positive screening tests for human immunodeficiency virus (HIV), hepatitis (Hep) B, Hep C, active tuberculosis or recent (< 2 week ago) clinically significant infection or evidence of active HIV, Hep B, or Hep C. (Note: If positive results are not indicative of true active or chronic infection, the patient can be treated.)
- Brain metastases considered unstable as: a. without confirmed stability over 60 days in patients previously treated with prior surgery or radiation; OR b. associated with symptoms and/or findings; OR c. requiring corticosteroids or anticonvulsants in the prior 60 days.
- Pregnant, planning to become pregnant, or breast feeding.
- Known allergy(ies) to any component of CMB305 or LV305.
- Men or women of reproductive ability who are unwilling to use effective contraception and women of childbearing potential who are unwilling to undergo pregnancy testing before and during the study.
- Clinically significant pulmonary dysfunction, as determined by medical history and physical exam. Patients so identified will undergo pulmonary functions testing and those with forced expiratory volume in 1 second (FEV1) < 2.0 L or carbon monoxide diffusing capability (DLco) (correlation for hemoglobin [corr for Hgb]) < 75% will be excluded.
- Significant cardiovascular abnormalities as defined by any one of the following: a. congestive heart failure, b. clinically significant hypotension, c. symptoms of coronary artery disease, d. presence of cardiac arrhythmias on electrocardiography (EKG) requiring drug therapy, e. ejection fraction < 50 % (dobutimine stress echo).
- Active and untreated central nervous system (CNS) metastasis.
- Autoimmune disease: patients with a history of inflammatory bowel disease are excluded from this study, as are patients with a history of autoimmune disease (e.g. systemic lupus erythematosus, vasculitis, infiltrating lung disease) whose possible progression during treatment would be considered by the investigator to be unacceptable.
- Steroids are not permitted 3 days prior to T cell infusion and concurrently during therapy.
- No prisoners or children will be enrolled on this study.

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03450122
Contact: Neeta Somaiah | 713-792-3626 | nsomaiah@mdanderson.org |
United States, Texas | |
M D Anderson Cancer Center | Recruiting |
Houston, Texas, United States, 77030 | |
Contact: Neeta Somaiah 713-792-3626 | |
Principal Investigator: Neeta Somaiah |
Principal Investigator: | Neeta Somaiah | M.D. Anderson Cancer Center |
Responsible Party: | M.D. Anderson Cancer Center |
ClinicalTrials.gov Identifier: | NCT03450122 |
Other Study ID Numbers: |
2017-0315 NCI-2018-00926 ( Registry Identifier: CTRP (Clinical Trial Reporting Program) ) 2017-0315 ( Other Identifier: M D Anderson Cancer Center ) P30CA016672 ( U.S. NIH Grant/Contract ) |
First Posted: | March 1, 2018 Key Record Dates |
Last Update Posted: | March 23, 2020 |
Last Verified: | March 2020 |
Studies a U.S. FDA-regulated Drug Product: | Yes |
Studies a U.S. FDA-regulated Device Product: | No |
Sarcoma Liposarcoma Sarcoma, Synovial Liposarcoma, Myxoid Neoplasms, Connective and Soft Tissue Neoplasms by Histologic Type Neoplasms Neoplasms, Adipose Tissue Neoplasms, Connective Tissue Aldesleukin Cyclophosphamide Interleukin-2 Immunosuppressive Agents Immunologic Factors Physiological Effects of Drugs |
Antirheumatic Agents Antineoplastic Agents, Alkylating Alkylating Agents Molecular Mechanisms of Pharmacological Action Antineoplastic Agents Myeloablative Agonists Analgesics, Non-Narcotic Analgesics Sensory System Agents Peripheral Nervous System Agents Anti-HIV Agents Anti-Retroviral Agents Antiviral Agents Anti-Infective Agents |