Dental Stem Cells and Bone Tissue Engineering (CELSORDINO) (CELSORDINO)
|The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.|
|ClinicalTrials.gov Identifier: NCT03194451|
Recruitment Status : Unknown
Verified June 2017 by Central Hospital, Nancy, France.
Recruitment status was: Not yet recruiting
First Posted : June 21, 2017
Last Update Posted : June 23, 2017
The main limitation in bone regeneration is the lack of vascularization of the newly shaped tissue. The main objective of this project is to check if the simultaneous differentiation of dental mesenchymal stem cells toward osteoblastic and endothelial lineage permits to obtain a new pre-vascularized tissue engineered bone construct.
We will then evaluate the boosting effect of the conditionned medium on cell differentiation and production of a prevascularized bone construct.
|Condition or disease||Intervention/treatment|
|Tooth, Impacted||Other: non-interventional study|
This project aims to develop a new pre-vascularized tissue engineered bone construct, using human cells of a simple and non invasive tissue source: dental pulp.
We will isolate mesenchymal stem cells from dental tissue of wisdom teeth, extracted from patients aged between 13 and 17 years old.
The objectives are: (i) to assess in combination the endothelial differentiation and osteoblastic differentiation of dental pulp stem cells (immature third molar); (ii) to study the boosting effect of the conditionned medium on cell differentiation and production of a prevascularized bone construct.
The differentiation will be monitored by evaluation of biosynthesized matrix, bone mineralization (immunohistochimie, Westen Blot), histological response (Von Kossa, Alizarin Red) and presence and quantification of pseudo-vascular structures (angiogenesis assay), compared to reference value (commercial differentiation medium).
|Study Type :||Observational|
|Estimated Enrollment :||40 participants|
|Official Title:||Dental Stem Cells and Bone Tissue Engineering|
|Estimated Study Start Date :||July 10, 2017|
|Estimated Primary Completion Date :||August 20, 2017|
|Estimated Study Completion Date :||July 10, 2019|
- Other: non-interventional study
- Bone mineralization [ Time Frame: Day 28 of cell culture ]Measured parameter is equal or superior to reference value (commercial differentiation medium)
- presence and quantification of pseudo-vascular structures [ Time Frame: Day 28 of cell culture ]Measured parameter is equal or superior to reference value (commercial differentiation medium)
Biospecimen Retention: Samples Without DNA
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03194451
|Contact: Vanessa MOBY, DD, PhD||0383154256 ext firstname.lastname@example.org|
|Principal Investigator:||Vanessa MOBY, DD, PhD||IMoPA, UMR-CNRS 7365 CNRS-UL, Medicine Faculty|