Apolipoprotein E (ApoE) and Metabolism
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|ClinicalTrials.gov Identifier: NCT03109561|
Recruitment Status : Recruiting
First Posted : April 12, 2017
Last Update Posted : May 28, 2018
|Condition or disease||Intervention/treatment|
|Cognitive Function Healthy||Other: Resting State Indirect Calorimetry Genetic: ApoE Diagnostic Test: Metabolism Other: Cognitive Challenge|
The investigator hopes determine whether a participant's version of ApoE affects their metabolic rate at rest and during a cognitive challenge. To determine this, the investigator will measure basal (resting) metabolic rates using a technique called indirect calorimetry (IC). This is accomplished by wearing a loose-fitting face mask that measures oxygen intake and carbon dioxide output.
To more accurately measure the amount of carbohydrates, fats and proteins that the body is metabolizing, participants will be asked to provide two urine samples (one at the beginning, and one at the end, of the study).
To determine what version of ApoE the participant inherited, the investigator will conduct a genetic test on DNA isolated from the participant's blood.
|Study Type :||Observational|
|Estimated Enrollment :||75 participants|
|Official Title:||APOE, Metabolism and Cognitive Function: An Assessment Via Indirect Calorimetry|
|Actual Study Start Date :||April 24, 2018|
|Estimated Primary Completion Date :||April 30, 2020|
|Estimated Study Completion Date :||April 30, 2020|
- Other: Resting State Indirect Calorimetry
The investigator will measure the amount of oxygen participants breathe in and the amount of carbon dioxide they breathe out using a portable unit. Participants will be asked wearing a loose-fitting face mask that measures oxygen intake and carbon dioxide output.
- Genetic: ApoE
The investigator will conduct a genetic test on DNA isolated from from the participants blood. Participants will be asked to provide about 2 tablespoons.
- Diagnostic Test: Metabolism
To more accurately measure the amount of carbohydrates, fats and proteins that the participants bodies metabolize, participants will be asked to provide two urine samples (one at the beginning, and one at the end, of the study). The total amount of urine to be collected is approximately 4 teaspoons.
- Other: Cognitive Challenge
Participants will be asked to follow simple instructions that are displayed on computer screen.
- Indirect Calorimetry [ Time Frame: 4 hours ]Measure resting state respiratory quotient in cognitively normal participants with various ApoE genotypes
- Respiratory Quotient (RQ) [ Time Frame: 4 hours ]Measure RQ during a cognitive challenge in cognitively normal individuals with various ApoE genotypes
- Urea Nitrogen Output [ Time Frame: 4 hours ]Improve RQ measure accuracy by determining urinary urea nitrogen output in collected urine samples from these subjects.
- Genotype [ Time Frame: 4 hours ]Genotype subjects using DNA isolated from collected blood samples.
Biospecimen Retention: Samples With DNA
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03109561
|Contact: Lance Johnson, PhD||8593232146||Johnson.Lance@uky.edu|
|United States, Kentucky|
|University of Kentucky Medical Center||Recruiting|
|Lexington, Kentucky, United States, 40536|
|Contact: Lance Johnson, PhD 859-323-2746 Johnson.Lance@uky.edu|
|Principal Investigator:||Lance Johnson1, PhD||University of Kentucky|