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Protein Requirements for Active Adolescent Females (IAAO-AF)

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT02800239
Recruitment Status : Completed
First Posted : June 15, 2016
Last Update Posted : April 1, 2019
Ajinomoto Co., Inc.
Information provided by (Responsible Party):
Daniel Moore, University of Toronto

Brief Summary:

Protein requirements in active individuals have been suggested to be greater than the current recommended dietary allowance (RDA).

Nutritional requirements for dietary amino acids in adolescents have traditionally been determined utilizing the nitrogen balance technique, which is prone to underestimating protein requirements. As a result, there is a need to re-evaluate recommendations in order to characterize how dietary amino acid needs may be modulated by physical activity.

Recent studies using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 50% higher than the RDA based on nitrogen balance data.

The purpose of this study is to measure the protein requirement in adolescent, females using the IAAO technique in the presence of a variable intensity exercise stimulus.

Condition or disease Intervention/treatment Phase
Increased Metabolic Requirement Healthy Dietary Supplement: Amino Acid Intake Not Applicable

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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 7 participants
Allocation: N/A
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Basic Science
Official Title: Evaluation of Protein Requirements in Active Adolescent Females
Actual Study Start Date : February 2016
Actual Primary Completion Date : October 2017
Actual Study Completion Date : December 31, 2017

Resource links provided by the National Library of Medicine

Arm Intervention/treatment
Experimental: Active, adolescent females
Subjects will receive different levels of amino acid intakes, varying from 0.2-2.67 g/kg/d
Dietary Supplement: Amino Acid Intake
Amino acid intakes will be varied at 0.2-2.67 g/kg/d

Primary Outcome Measures :
  1. 13CO2 ([13]Carbon Dioxide) Excretion (µmol/kg/h) [ Time Frame: 4 hours/ study day ]

Secondary Outcome Measures :
  1. [13C]Phenylalanine Oxidation (µmol/kg/h) [ Time Frame: 4 hours/ study day ]

Information from the National Library of Medicine

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Ages Eligible for Study:   10 Years to 15 Years   (Child)
Sexes Eligible for Study:   Female
Accepts Healthy Volunteers:   Yes

Inclusion Criteria (Adolescents):

  • Healthy, active adolescent who regularly participates in moderate to vigorous physical activity 5 times a week for a minimum of 1 hour.
  • Being -0.5 to +1.0 years from peak height velocity, determined by the ratio of standing to sitting height, with the maximal age for adolescents being 16 years.

Obtaining a minimum level of 5.8 on the Beep Test

Exclusion Criteria (All Subjects):

  • Inability to meet health and physical activity guidelines according to the Physical Activity Readiness Questionnaire (PAR-Q+) and Physical Activity Questionnaire,
  • An inability to adhere to any of the protocol guidelines (i.e. caffeine consumption)
  • A biological age falling outside of the aforementioned ranges

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT02800239

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Canada, Ontario
Goldring Centre for High Performance Sport
Toronto, Ontario, Canada, M5S2C9
Sponsors and Collaborators
University of Toronto
Ajinomoto Co., Inc.
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Principal Investigator: Daniel Moore, PhD University of Toronto
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Responsible Party: Daniel Moore, Assistant Professor, University of Toronto Identifier: NCT02800239    
Other Study ID Numbers: IAAO-AF
First Posted: June 15, 2016    Key Record Dates
Last Update Posted: April 1, 2019
Last Verified: March 2019
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No