Safety of Intra-Articular Sc-rAAV2.5IL-1Ra in Subjects With Moderate Knee OA (AAVIL-1Ra)
![]() |
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. |
ClinicalTrials.gov Identifier: NCT02790723 |
Recruitment Status :
Completed
First Posted : June 6, 2016
Last Update Posted : April 15, 2022
|
- Study Details
- Tabular View
- No Results Posted
- Disclaimer
- How to Read a Study Record
Condition or disease | Intervention/treatment | Phase |
---|---|---|
Osteoarthritis, Knee | Drug: sc-rAAV2.5IL-1Ra | Phase 1 |
Study Type : | Interventional (Clinical Trial) |
Actual Enrollment : | 9 participants |
Allocation: | Non-Randomized |
Intervention Model: | Parallel Assignment |
Masking: | None (Open Label) |
Primary Purpose: | Treatment |
Official Title: | A Phase I Study Evaluating the Safety of Intra-Articular Sc-rAAV2.5IL-1Ra in Subjects With Moderate Osteoarthritis of the Knee |
Actual Study Start Date : | June 5, 2019 |
Actual Primary Completion Date : | March 17, 2022 |
Actual Study Completion Date : | March 17, 2022 |

Arm | Intervention/treatment |
---|---|
Experimental: Low Dose Group
Subjects will receive a single intra-articular 2mL injection consisting of 10{11} viral genomes.
|
Drug: sc-rAAV2.5IL-1Ra
Self-complementing, recombinant AAV carrying IL-1Ra cDNA produced by the Research Institute at Nationwide Children's Hospital.
Other Names:
|
Experimental: Medium Dose Group
Subjects will receive a single intra-articular 2mL injection consisting of 10{12} viral genomes.
|
Drug: sc-rAAV2.5IL-1Ra
Self-complementing, recombinant AAV carrying IL-1Ra cDNA produced by the Research Institute at Nationwide Children's Hospital.
Other Names:
|
Experimental: High Dose Group
Subjects will receive a single intra-articular 2mL injection consisting of 10{13} viral genomes.
|
Drug: sc-rAAV2.5IL-1Ra
Self-complementing, recombinant AAV carrying IL-1Ra cDNA produced by the Research Institute at Nationwide Children's Hospital.
Other Names:
|
- Number of subjects experiencing severe adverse events. [ Time Frame: 53 weeks ]Assess the local and systemic safety of three different doses of sc-rAAV2.5IL-1Ra delivered by intra-articular injection into one knee joint of patients with moderate OA of the knee.

Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies.
Ages Eligible for Study: | 18 Years to 80 Years (Adult, Older Adult) |
Sexes Eligible for Study: | All |
Accepts Healthy Volunteers: | No |
Inclusion Criteria
- Age 18-80 years
- Gender: both males and females
- Target disease: Moderate OA of the knee
- Persistent symptoms, despite standard NSAID
- Absence of clinically significant abnormal values for the following:
- Complete blood count
- Prothrombin Time, Activated partial thromboplastin time
- Blood chemistry (Glucose, Na, K, Cl, CO2, BUN, creatinine, Ca, PO4, magnesium, uric acid)
- Liver function tests (amylase, total bilirubin, alkaline phosphate, GGT, AST, ALT, total protein, albumin)
- Able and willing to return to the Mayo Clinic for follow-up visits, as required by this study
- Able undergo MRI of the knee
- Subjects should be able to give appropriate consent.
- Potential subjects should have failed a three-month trial of a minimum of two conservative therapies before being considered for this trial. These conservative therapies include: activity modification, weight loss, physical therapy, and anti-inflammatory or injection therapy.
Exclusion Criteria
- Pregnant, or currently breast-feeding
- Ongoing infectious disease including HIV, HTLV, hepatitis, syphilis or tuberculosis positive
- Individuals who have OA as part of another syndrome (e.g. Ehler's Danlos, Stickler syndrome, etc.)
- Systemic, rheumatic or inflammatory disease of the knee or chondrocalcinosis, hemochromatosis, inflammatory arthritis, arthropathy of the knee associated with juxta-articular Paget's disease of the femur or tibia, ochronosis, hemophilic arthropathy, infectious arthritis, Charcot's knee joint, villonodular synovitis, and synovial chondromatosis.
- Clinically significant cardiovascular, renal, hepatic, endocrine disease, diabetes, cancer, autoimmune diseases; a serious infection or major operation within 30 days of enrollment; a history of untreated psychiatric disease or recent history of alcoholism or drug addiction.
- Currently taking immunosuppressant medications
- Anticipated major surgery during the study period.
- Individuals involved in another interventional protocol, or have been treated under one within the last 3 months.
- Intra-articular therapy in the index knee within the previous 3 months.
- Surgery to the target knee within 6 months prior to screening.
- Surgery to other weight bearing joints if it will interfere with knee assessments
- Prior articular transplant procedures
- Orthopedic hardware or implantable devices in the index knee
- Prior reconstruction surgery to the target knee within 12 months
- X-ray findings of acute fractures
- Known severe loss of bone density, and/or severe bone or joint deformity in the target knee.
- Significant target knee infection or overlying skin disorder/infection within the previous 6 months prior to study enrollment
- Require cane or other assistive device for walking
- Symptomatic OA of the hips, spine or ankle if it would interfere with the evaluation of the target knee,
- History of documented nerve damage in the affected limb, or vascular insufficiency,
- Condition requiring use of systemic steroids,
- Coagulation disorder.
- Patients with unstable knees
- Temperature above 99.5o F.
- Identification as a member of a vulnerable population.
- BMI greater than 40.
- History of allergy to local anesthetics
- Currently taking anti-rheumatic disease medication (including methotrexate or other antimetabolites) within the 3 months prior to entry in the study.
- Any illness or condition which, in the investigators' judgement will interfere with the patient's ability to comply with the protocol, compromise patient safety, or interfere with the interpretation of the study results

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02790723
United States, Minnesota | |
Mayo Clinic | |
Rochester, Minnesota, United States, 55905 |
Principal Investigator: | Christopher H. Evans, Ph.D. | Mayo Clinic |
Publications automatically indexed to this study by ClinicalTrials.gov Identifier (NCT Number):
Responsible Party: | Christopher H. Evans, Ph.D., PI, Mayo Clinic |
ClinicalTrials.gov Identifier: | NCT02790723 |
Other Study ID Numbers: |
15-007542 |
First Posted: | June 6, 2016 Key Record Dates |
Last Update Posted: | April 15, 2022 |
Last Verified: | April 2022 |
Individual Participant Data (IPD) Sharing Statement: | |
Plan to Share IPD: | No |
Studies a U.S. FDA-regulated Drug Product: | Yes |
Studies a U.S. FDA-regulated Device Product: | No |
Osteoarthritis Osteoarthritis, Knee Arthritis Joint Diseases |
Musculoskeletal Diseases Rheumatic Diseases Interleukin 1 Receptor Antagonist Protein Antirheumatic Agents |