A New Micro Swim-up Procedure for Sperm Preparation in ICSI Treatment
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ClinicalTrials.gov Identifier: NCT02672124
Recruitment Status : Unknown
Verified February 2018 by Palini Simone, Cervesi Hospital, Cattolica, Italy. Recruitment status was: Recruiting
The aim of this study is to evaluate the microbiological safety and the efficacy of a new not expensive, easy and fast technique (Direct Microswimup) for sperm preparation in In Vitro Fertilization (IVF) treatments without the use of centrifuge, able to simplify the procedure and to avoid mismatches by ensuring the same results of the standard techniques in terms of fertilization rate, blastulation rate, pregnancy/abortion rate, cryostorage rate, and a better euploid status of the embryos.
A standard bacteriological culture [based on inoculation of blood and "chocolate" agar plates and Brain-Heart Infusion broth, followed by incubation at 37°C for up to 72 hours] was performed in order to investigate the presence and amount of colonizing bacteria delivered with the semen at ejaculation and after treatment
Secondary Outcome Measures :
semen treatment efficacy [ Time Frame: 6 months ]
number of obtain blastocysts (%) and number of pregnancy (%)
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Layout table for eligibility information
Ages Eligible for Study:
20 Years to 35 Years (Adult)
Sexes Eligible for Study:
Accepts Healthy Volunteers:
This study included couples submitted to in vitro fertilization treatment at the Unit of Physiopathology of Reproduction (Cervesi Hospital, Cattolica, RN, Italy).
at least 8 oocytes retrieved at pickup
oocyte insemination with ICSI technique
only elective single embryo transfer (eSET) at blastocyst stage
sperm concentration ≥ 1 X 106 /ml
absence of antibiotic treatment in the previous four weeks for male patients