Measuring Protein Requirement Using Amino Acid Oxidation in Strength and Endurance Athletes
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|ClinicalTrials.gov Identifier: NCT02621294|
Recruitment Status : Unknown
Verified November 2015 by Peter Lemon, University of Western Ontario, Canada.
Recruitment status was: Recruiting
First Posted : December 3, 2015
Last Update Posted : December 3, 2015
|Condition or disease||Intervention/treatment||Phase|
|Protein Requirement of Athletes||Other: Measuring protein requirement||Not Applicable|
|Study Type :||Interventional (Clinical Trial)|
|Estimated Enrollment :||16 participants|
|Intervention Model:||Single Group Assignment|
|Masking:||None (Open Label)|
|Official Title:||Measuring Protein Requirement Using the Indicator Amino Acid Oxidation Method in Healthy Young Strength and Endurance Exercise Trained Individuals|
|Study Start Date :||November 2015|
|Estimated Primary Completion Date :||December 2016|
|Estimated Study Completion Date :||December 2016|
Experimental: Measuring protein requirement
Measuring protein requirement of athletes by feeding different amount of protein in the form of amino acid mixture and measuring their oxidation through expired CO2
Other: Measuring protein requirement
a repeated measures design will be used on each athlete receiving eight graded intakes of protein on separate days in the form of AA in random order ranging from 0.2 to 3.5 g/(kg/d). Three baseline samples of breath and urine will be collected at 60, 45 and 30 min before the stable isotope is given orally. During a period of 150 to 270 min. after stable isotope ingestion, 5 breath and urine samples (every 30 min) will be collected to determine an isotopic plateau in breath and urine. The rate of release of 13CO2 from the oxidation of L-[1-13C] phenylalanine (F 13CO2) in breath will be measured and a breakpoint analysis will determine total protein requirement
- measurement of protein requirement as a response of 13C oxidation (µmol . kg-1 . h-1) of 13C-Phenylalanine to varied graded intakes of protein (g/kg/d) in form of amino acid mixture based on egg protein profile [ Time Frame: within 8 hours of feeding period ]Oxidation of ingested L-[1-13C] phenylalanine (µmol . kg-1 . h-1) to 13CO2 (µmol . kg-1 . h-1) in response to graded intakes of protein will be measure and after determination of a plateau in oxidation curve (using change point regression analysis), break point will be defined as g/kg/d protein requirement.
- Measuring body composition (percent fat and fat free mass) using air displacement plethysmography (BOD POD) [ Time Frame: 10 minutes fasted in the morning ]Participants will report fasted in the morning for the measurement of body fat and fat-free mass percent (%) using a BOD POD.
- Measuring resting energy expenditure (kcal/d) using an open-circuit indirect calorimetry [ Time Frame: 40 minutes fasted in the morning ]Resting energy expenditure (kcal/d) of all participants will be measured using an indirect calorimetry for duration of 40 min. by laying flat on a bed.
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02621294
|Contact: Peter Lemon, PhD||519 661-2111 ext email@example.com|
|Contact: Arash Bandegan, MScfirstname.lastname@example.org|
|Exercise Nutrition Research Laboratory||Recruiting|
|London, Ontario, Canada, N6A 5B9|
|Contact: Peter WR Lemon, PhD 519-661-2111 ext 88139 email@example.com|
|Principal Investigator: Peter Lemon, PhD|
|Sub-Investigator: Arash Bandegan, MSc.|
|Principal Investigator:||Peter Lemon, PhD||Western University|