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Involvement of the Translation Initiation Factors in Resolution of Inflammation in the Elderly Population

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ClinicalTrials.gov Identifier: NCT02519361
Recruitment Status : Unknown
Verified August 2015 by Meir Medical Center.
Recruitment status was:  Not yet recruiting
First Posted : August 10, 2015
Last Update Posted : August 10, 2015
Sponsor:
Information provided by (Responsible Party):
Meir Medical Center

Brief Summary:

Aging cause specific changes in the immune system. Processes like "immunoessence" and "inflammaging" offend the functioning of the immune cells and expose the elderly patient to infections that can lead to morbidity and death.

Protein translation regulation offers a strategic advantage to the immune cells, because it enables rapid activation or termination of synthesis of specific proteins, required for inflammation or its resolution. Translation initiation depends on recruitment of eukaryotic initiation factor "eIF4F" complex.

The aim of the current study is to investigate the involvement of the translation initiation factors (eIF4E and eIF4G) in the process of recovery from acute infection in elderly patient admitted to the internal department with an acute infection.


Condition or disease
Infection in the Elderly

Detailed Description:
  1. Blood samples of elderly patients diagnosed with acute infection (fever and leukocytosis) will be collected twice - at admittance to Meir hospital and after recovery (24-48 hours without fever and leukocytosis). evidence of infection (chest x ray, urine and blood samples) will be collected. participants will be divided to two age groups: 65-80 yrs and very old patient >85 yrs, and will be compared to younger patients aged 50-65.
  2. Peripheral blood samples will be separated to subpopulations of lymphocytes, monocytes and polymorphonuclears, and protein will be extracted.
  3. protein lysates will be separated by SDS-PAGE electrophoresis and immunoblotted for phosphorylated and total eIF4E and eIF4G; their regulators: Mnk 1/2, 4EBP1, mTOR and targets: cyclin D1, c-Myc, survivin, BCL2, VEGFA, etc.
  4. Paired samples (within each age group) will be analyzed and correlated with clinical response of the patients. The source of the infection will also be considered.
  5. Comparison between age groups at admittance and upon recovery will be made (Unpaired).

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Study Type : Observational
Estimated Enrollment : 30 participants
Observational Model: Cohort
Time Perspective: Prospective
Official Title: The Involvement of the Translation Initiation Factors eIF4E and eIF4G in Resolution of Inflammation in the Elderly Population
Study Start Date : August 2015
Estimated Primary Completion Date : January 2016
Estimated Study Completion Date : January 2016



Primary Outcome Measures :
  1. Translation initiation factors expression by immunoblotting, in leukocytes sub population [ Time Frame: 1 week ]
    Assessment of eIF4E and eIF4G expression by immunoblotting, in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation, in three groups of age


Secondary Outcome Measures :
  1. Translation initiation factors regulators expression by immunoblotting in leukocytes sub population [ Time Frame: 1 week ]
    Assessment of eIF4E and eIF4G regulators expression by immunoblotting, in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation in three age groups

  2. Translation initiation factors targets expression by immunoblotting in leukocytes sub population [ Time Frame: 1 week ]
    Assessment of eIF4E and eIF4G targets expression by immunoblotting in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation, in three age groups



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Ages Eligible for Study:   50 Years and older   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Probability Sample
Study Population
patients admitted to internal department due to acute infection
Criteria

Inclusion Criteria:

  • patient aged 50 y and more with:
  • fever >38c that started in the 48 hours prior to admission
  • leukocytosis >10.0 K/microLiter
  • suspected source of infection by symptoms, lab results or imaging
  • treatment with antibiotics started in the last 24 hours or is about to be started

Exclusion Criteria:

  • patients who cannot sign the informed concent ( dementia)
  • patients with active malignancy
  • patients who are under chemotherapy

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02519361


Contacts
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Contact: rachel heffez ayzenfeld, MD 09 7472185 rachelhe2@clalit.org.il

Sponsors and Collaborators
Meir Medical Center
Investigators
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Principal Investigator: Rachel Heffez Ayzenfeld, MD r.h.ayzenfeld
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Responsible Party: Meir Medical Center
ClinicalTrials.gov Identifier: NCT02519361    
Other Study ID Numbers: 0148-15-MMC
First Posted: August 10, 2015    Key Record Dates
Last Update Posted: August 10, 2015
Last Verified: August 2015
Keywords provided by Meir Medical Center:
elderly
immune response
acute infection
Additional relevant MeSH terms:
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Inflammation
Pathologic Processes