Involvement of the Translation Initiation Factors in Resolution of Inflammation in the Elderly Population
|The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.|
|ClinicalTrials.gov Identifier: NCT02519361|
Recruitment Status : Unknown
Verified August 2015 by Meir Medical Center.
Recruitment status was: Not yet recruiting
First Posted : August 10, 2015
Last Update Posted : August 10, 2015
Aging cause specific changes in the immune system. Processes like "immunoessence" and "inflammaging" offend the functioning of the immune cells and expose the elderly patient to infections that can lead to morbidity and death.
Protein translation regulation offers a strategic advantage to the immune cells, because it enables rapid activation or termination of synthesis of specific proteins, required for inflammation or its resolution. Translation initiation depends on recruitment of eukaryotic initiation factor "eIF4F" complex.
The aim of the current study is to investigate the involvement of the translation initiation factors (eIF4E and eIF4G) in the process of recovery from acute infection in elderly patient admitted to the internal department with an acute infection.
|Condition or disease|
|Infection in the Elderly|
- Blood samples of elderly patients diagnosed with acute infection (fever and leukocytosis) will be collected twice - at admittance to Meir hospital and after recovery (24-48 hours without fever and leukocytosis). evidence of infection (chest x ray, urine and blood samples) will be collected. participants will be divided to two age groups: 65-80 yrs and very old patient >85 yrs, and will be compared to younger patients aged 50-65.
- Peripheral blood samples will be separated to subpopulations of lymphocytes, monocytes and polymorphonuclears, and protein will be extracted.
- protein lysates will be separated by SDS-PAGE electrophoresis and immunoblotted for phosphorylated and total eIF4E and eIF4G; their regulators: Mnk 1/2, 4EBP1, mTOR and targets: cyclin D1, c-Myc, survivin, BCL2, VEGFA, etc.
- Paired samples (within each age group) will be analyzed and correlated with clinical response of the patients. The source of the infection will also be considered.
- Comparison between age groups at admittance and upon recovery will be made (Unpaired).
|Study Type :||Observational|
|Estimated Enrollment :||30 participants|
|Official Title:||The Involvement of the Translation Initiation Factors eIF4E and eIF4G in Resolution of Inflammation in the Elderly Population|
|Study Start Date :||August 2015|
|Estimated Primary Completion Date :||January 2016|
|Estimated Study Completion Date :||January 2016|
- Translation initiation factors expression by immunoblotting, in leukocytes sub population [ Time Frame: 1 week ]Assessment of eIF4E and eIF4G expression by immunoblotting, in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation, in three groups of age
- Translation initiation factors regulators expression by immunoblotting in leukocytes sub population [ Time Frame: 1 week ]Assessment of eIF4E and eIF4G regulators expression by immunoblotting, in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation in three age groups
- Translation initiation factors targets expression by immunoblotting in leukocytes sub population [ Time Frame: 1 week ]Assessment of eIF4E and eIF4G targets expression by immunoblotting in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation, in three age groups
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02519361
|Contact: rachel heffez ayzenfeld, MD||09 email@example.com|
|Principal Investigator:||Rachel Heffez Ayzenfeld, MD||r.h.ayzenfeld|