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Trial record 24 of 108 for:    CALCIUM CATION

Expression and Sequence of Calcium Channels in Non-small Cell Lung Carcinoma (NSCLC)

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ClinicalTrials.gov Identifier: NCT02283021
Recruitment Status : Unknown
Verified August 2015 by Hadassah Medical Organization.
Recruitment status was:  Enrolling by invitation
First Posted : November 5, 2014
Last Update Posted : August 20, 2015
Sponsor:
Information provided by (Responsible Party):
Hadassah Medical Organization

Brief Summary:

Calcium is an extremely important ion used in our body for many processes. One of its tasks is to control gene expression. Cells intake calcium from their surroundings though special calcium channels located on the surface membranes of the cells.

The great many studies on such calcium channels were performed on excitable cells such as muscle, heart or neuronal cells, where the calcium ions are controlled by voltage. Surprisingly, not much is known about the identity of calcium subunits in non-excitable cells like epithelial cells (which compose most of the connective tissue in the body), liver cells, lung cells, immune system cells, etc.

Recently, the investigators have shown that calcium channels from muscles are, in fact, expressed in T cells of the immune system, where they are used for proliferation. The investigators postulated that probably other cell types, especially cancerous cell types, might be using these subunits similarly.

The aim of this study is to determine the identity and sequence of calcium subunits expressed in non-small cell Lung Carcinoma (NSCLC), which accounts for 80% of the worldwide lung cancer deaths.


Condition or disease Intervention/treatment
NSCLC Other: A biopsy sample

Detailed Description:

Initial examination of NSCLC cell lines determined that they express the mRNA of voltage gated calcium subunits, found normally in neurons. Furthermore sequence analysis of these subunits has shown that modifications have occurred.

The aim of the study in NSCLC patients is to sequence and clone the calcium channel subunits expressed in their tumor. Expression and Sequence will be compared between the normal and cancerous lung tissue for each patient.


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Study Type : Observational
Estimated Enrollment : 200 participants
Observational Model: Cohort
Time Perspective: Prospective
Official Title: Expression and Sequence of Calcium Channels in Non-small Cell Lung Carcinoma (NSCLC)
Study Start Date : January 2015
Estimated Primary Completion Date : January 2017
Estimated Study Completion Date : January 2018

Resource links provided by the National Library of Medicine


Group/Cohort Intervention/treatment
NSCLC Patients
Sample biopsies from normal and cancerous lung tissue.
Other: A biopsy sample
Sample tissue will be obtained from the routine biopsy taken for pathological evaluation. A pathologist will determine which part of the tissue will be forwarded for this study, to avoid any interference with the pathological evaluation.

Patients without NSCLC
Sample biopsies from normal lung tissue.Control group.
Other: A biopsy sample
Sample tissue will be obtained from the routine biopsy taken for pathological evaluation. A pathologist will determine which part of the tissue will be forwarded for this study, to avoid any interference with the pathological evaluation.




Primary Outcome Measures :
  1. mRNA expression and sequence of calcium channel subunits in NSCLC [ Time Frame: up to 1 month per patient ]
    mRNA will be extracted from each sample biopsy followed by real time PCR analysis. If any calcium subunit is detected, semi quantitative PCR will be performed, PCR bands will be cloned and sequenced.


Biospecimen Retention:   Samples Without DNA
Lung specimen biospsies are routinely taken from NSCLC patients and sent to pathological evaluation. We will obtain small samples from these biopsies from the pathologist, which are normally discarded. mRNA will be extracted from these samples.


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Ages Eligible for Study:   18 Years to 80 Years   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Sampling Method:   Probability Sample
Study Population
Patients diagnosed with NSCLC and patients not diagnosed with NSCLC
Criteria

Inclusion Criteria:

  • NSCLC patients
  • Patients without NSCLC
  • Men or woman between 18-80 years of age

Exclusion Criteria:

  • Pregnant woman

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02283021


Sponsors and Collaborators
Hadassah Medical Organization
Investigators
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Study Director: Hadas Lamberg, PhD Hadassah Medical Organization

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Responsible Party: Hadassah Medical Organization
ClinicalTrials.gov Identifier: NCT02283021     History of Changes
Other Study ID Numbers: CALCIUM-HMO-CTIL
First Posted: November 5, 2014    Key Record Dates
Last Update Posted: August 20, 2015
Last Verified: August 2015
Keywords provided by Hadassah Medical Organization:
expression of L-type calcium subunits
Additional relevant MeSH terms:
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Calcium, Dietary
Calcium
Calcium-Regulating Hormones and Agents
Carcinoma, Non-Small-Cell Lung
Lung Neoplasms
Neoplasms
Carcinoma, Bronchogenic
Bronchial Neoplasms
Respiratory Tract Neoplasms
Thoracic Neoplasms
Neoplasms by Site
Lung Diseases
Respiratory Tract Diseases
Physiological Effects of Drugs
Bone Density Conservation Agents