Glycosylated Ferritin in Macrophagic Activation Syndromes (FERRITGLY01)
|The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Know the risks and potential benefits of clinical studies and talk to your health care provider before participating. Read our disclaimer for details.|
|ClinicalTrials.gov Identifier: NCT02143986|
Recruitment Status : Recruiting
First Posted : May 21, 2014
Last Update Posted : April 17, 2019
In healthy subjects, from 50 to 80 % of the serum ferritin is glycosylated [1, 2] . A decrease in the percentage of ferritin glycosylation can be observed in inflammatory diseases, malignancies, infections, or liver disease but is rarely less than 20% [3 , 4] . Percentage of glycosylated ferritin below 20% have been described in patients with adult Still's disease and haemophagocytosis lymphohistiocytic syndromes (HLH).
The glycosylated ferritin has been included in the diagnostic criteria for Still's disease in adults. A cut-off of less than 20 % has a sensitivity and specificity of 72 and 69 % respectively , and 35 and 94 % when combined with a total ferritin level greater than 5 times normal value. This parameter was also suggested to be a more specific marker to confirm a diagnosis of HLH than a high ferritin level ( > 500μg / L). However, several limitations of this parameter were highlighted, some conditions making its interpretation difficult : particularly in cases of major hepatic cytolysis and severe sepsis (miliary tuberculosis, lymphoma and disease Adult Still).
It is not always possible to distinguish severe sepsis, HLH syndrome and Still's disease.
A fine analysis of various glycoforms components of ferritin could be used to distinguish different subgroups of patients. Few data are available on the mechanism of secretion and glycosylation of ferritin, but the investigators assume that the glycosylation patterns of ferritin may vary between different disease states and reflect distinct underlying pathophysiological mechanisms.
|Condition or disease|
|Still's Disease, Adult-Onset Sepsis Macrophagic Activation Syndrome|
The objectives of this study are:
- . To evaluate the diagnostic performance of the assay of the glycosylated ferritin under HLH syndrome and Still's disease in adults.
- . To study the different glycoforms of ferritin from serum of patient with hyperferritinemia.
Materials and methods
Patient groups studied and control group
- Any suspicion of HLH or Still's disease syndrome in adults
Control groups: sepsis, severe sepsis, septic shock, inflammation ( lupus, rheumatoid arthritis), severe hepatic necrosis, hematologic malignancy at diagnosis or relapse
2 . Samples
- Serum will be collected for the determination of glycosylated ferritin in patients with suspicion of HLH syndrome or Still's disease in adults
For the orther patients remaining serum samples will be collected from ferritin sample of the routine biological evaluation in patients with infammatory diseases ( lupus, rheumatoid arthritis) , hematologic malignancies at diagnosis or relapse, hepatic necrosis or sepsis.
3 . Characterization of patients based on diagnostic criteria (clinical and biological) retained (with collaborating physicians).
After approval of the EC CHU Brugmann, the protocol will be submitted to other EC institutions.
4 . Analysis of the glycosylation profile of ferritin by mass spectrometry. (in collaboration with the Faculty of Pharmacy , Department of Pharmaceutical Chemistry , Van Antwerpen P and Delporte C)
Analysis and interpretation
- . Determination of the sensitivity and specificity of the diagnostic assay of the glycosylated ferritin under HLH syndrome and Still's disease .
- . Characterization of subgroups of patients according to the following information: Ferritin and its glycosylated fraction, documented infections , fever, MOF , hepatomegaly, splenomegaly, haemophagocytosis (when bone marrow aspirate available), thrombocytosis, anemia, leukopenia, neutropenia, liver enzymes, hypertriglyceridemia, coagulopathy, hypofibrinogenemia, VS / CRP.
- . Analysis of glycosylation patterns according to the patient subgroup.
|Study Type :||Observational|
|Estimated Enrollment :||60 participants|
|Official Title:||Glycosylated Ferritin in Differential Diagnosis of Still's Disease, Sepsis and Other Macrophagic Activation Syndromes.|
|Study Start Date :||May 2014|
|Estimated Primary Completion Date :||December 2020|
|Estimated Study Completion Date :||December 2020|
|Macrophagic activation syndrome|
- Fraction of glycosylated ferritin in serum [ Time Frame: One year ]
Biospecimen Retention: Samples Without DNA
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02143986
|Contact: Francis Corazza, MD, PhDfirstname.lastname@example.org|
|Contact: France Debaugnies, Pharmemail@example.com|
|Brugmann University Hospital||Recruiting|
|Brussels, Belgium, 1020|
|Contact: Francis Corazza, MD PhD firstname.lastname@example.org|
|Contact: France Debaugnies, Pharm email@example.com|
|Principal Investigator: francis Corazza, MD PhD|