Smoking Influence on Apoptosis in Periodontitis
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|ClinicalTrials.gov Identifier: NCT02111005|
Recruitment Status : Completed
First Posted : April 10, 2014
Last Update Posted : March 16, 2016
Apoptosis is an evolutionary form of physiological cell death. Studies suggest that apoptosis is involved in the pathogenesis of periodontal diseases. Human gingival fibroblasts (HGF) have an important role in the periodontal immune response. It is believed that HGF can be diminished and/or eliminated by means of apoptosis.
Smoking is one of the most common risk factor of periodontal disease. Studies indicated that smoking can increase the risk of periodontitis by enhancing the apoptosis of gingival fibroblast.
The purpose of this study is to determine and to investigate apoptosis of HGF in gingival biopsies collected from smokers and non smokers who are diagnosed with chronic periodontitis or aggressive periodontitis.
Eighty subjects will be invited to participate in this study. Patients will be allocated into four groups (20 patients each). Gingival biopsies will be obtained from the base of papillae during surgical treatment (open flap curettage) and will be examined by Immuno-histochemical analysis. Immune-staining will be done using p53 monoclonal mouse anti-human antibody.
|Condition or disease|
|Chronic Periodontitis Aggressive Periodontitis|
|Study Type :||Observational|
|Actual Enrollment :||80 participants|
|Official Title:||Influence of Smoking on Fibroblast Apoptosis in Patients With Chronic and Aggressive Periodontitis|
|Study Start Date :||April 2015|
|Actual Primary Completion Date :||December 2015|
|Actual Study Completion Date :||March 2016|
Smoking Aggressive Periodontitis group
This group comprises 20 patients who are smokers and aged < 35 years and diagnosed with rapid attachment loss with periodontal pocket depth (PD) > 4 mm around at least three teeth other than the first molars and incisors. Rapid bone destruction (>50%bone loss at diseased sites). Weak relationship between dental plaque and the severity of gingival inflammation.
Smoking Chronic Periodontitis group
This group comprises 20 patients who are smokers and aged > 45 years and have presence of ≥2 non-adjacent sites per quadrant that were not first molars or incisors, with probing depth (PD) ≥5 mm, which bleed on gentle probing. The demonstrated radiographic bone loss ≥30% of the root length, patient with poor oral hygiene, the amount of accumulated plaque commensurate with the amount of clinical attachment level (CAL)
Non-smoking Aggressive Periodontitis grp
This group comprises 20 patients who are age- and sex- matched to the 'Smoking Aggressive Periodontitis group' and are non-smokers suffering from aggressive periodontitis.
Non-smoking Chronic periodontitis grp
This group comprises 20 patients who are age- and sex-matched to the 'Smoking Chronic Periodontitis group' and are non-smokers suffering from chronic periodontitis.
- P53 levels in gingival biopsy samples [ Time Frame: The measurement will be performed at T0 (baseline measurement) once the sample has been recruited ]This variable is going to be measured by an immunohistochemical analysis
- presence or absence of fibroblasts' apoptosis in the gingival tissues [ Time Frame: The measurement will be performed at T0 (baseline measurement) once the sample has been recruited ]this variable is going to be measured by an immunohistochemical analysis
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02111005
|Syrian Arab Republic|
|Ali Abou Sulaiman|
|Damascus, Syrian Arab Republic, DM20AM18|
|Department of Periodontics, University of Damascus Dental School|
|Damascus, Syrian Arab Republic, DM20AM18|
|Principal Investigator:||Ali Abou Sulaiman, DDS MSc PhD||University of Damascus Dental School, Department of Periodontology|
|Study Director:||Sharif Barakat, DDS MSc Phd||University of Damascus Dental School, Department of Periodontology|