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Macrophages Effect on Chemoresistance

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ClinicalTrials.gov Identifier: NCT01921699
Recruitment Status : Unknown
Verified August 2013 by SHOROOK NA'ARA MD, Rambam Health Care Campus.
Recruitment status was:  Not yet recruiting
First Posted : August 13, 2013
Last Update Posted : August 13, 2013
Sponsor:
Information provided by (Responsible Party):
SHOROOK NA'ARA MD, Rambam Health Care Campus

Brief Summary:

Macrophages are derived from monocytes recruited to the tumor site and stimulated by specific chemokines secreted by tumor cells. These tumor associated macrophages (TAMs) have been postulated as being involved in the progression of cancer.

Based on our preliminary findings and on published data we hypothesized that macrophage-induced chemoresistance (MIC) can promote survival of pancreatic carcinoma cells during chemotherapy.

The overall goal of this study is to evaluate the mechanism of MIC in an in-vitro model of Pancreatic ductal adenocarcinoma (PDA).

methods:

  1. The human PDA cell line Panc1 will be grown in suitable conditions.
  2. Macrophages will be produced by incubating mononuclear cells from the blood of healthy donors in medium with M-CSF for 7 days.
  3. TAMs will be generated by culturing these macrophages with tumor-culture conditioning medium (TCCM) of PDA Cells for an additional 72 hours.
  4. Human pancreatic cells (PANC1) will be treated with gemcitabine following exposure to macrophages CM.
  5. Cell proliferation will be quantified by light microscopy and by an XTT Cell Proliferation Assay Kit.

Condition or disease Intervention/treatment Phase
the Focus of the Study is Macrophage-induced Chemoresistance in Pancreatic Carcinoma Cells During Chemotherapy. Procedure: taking blood samples from healthy people Not Applicable

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Study Type : Interventional  (Clinical Trial)
Estimated Enrollment : 2 participants
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Basic Science
Official Title: Impact of Macrophages on De-Novo Chemoresistance of Adenocarcinoma Cells
Study Start Date : August 2013
Estimated Primary Completion Date : September 2013

Resource links provided by the National Library of Medicine


Arm Intervention/treatment
irrelevant Procedure: taking blood samples from healthy people



Primary Outcome Measures :
  1. proliferation and migration of PANC1 cell line in the tested groups. [ Time Frame: one day to get the blood samples, and a year after that to complete the basic research ]
    the macrophages will be isolated from the blood we'll get from the healthy volunteers. the outcome measure is related to the basic research and not clinical so there's no symptom/outcome related to the participants.


Secondary Outcome Measures :
  1. To determine whether direct cell-to-cell interaction or soluble factors participate in MIC. [ Time Frame: a year from obtaining the blood samples ]


Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 40 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • healthy people

Exclusion Criteria:

  • refusal to participate

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Responsible Party: SHOROOK NA'ARA MD, MD PhD student, Rambam Health Care Campus
ClinicalTrials.gov Identifier: NCT01921699     History of Changes
Other Study ID Numbers: pending
pending ( Other Identifier: Rambam medical health campus )
First Posted: August 13, 2013    Key Record Dates
Last Update Posted: August 13, 2013
Last Verified: August 2013

Keywords provided by SHOROOK NA'ARA MD, Rambam Health Care Campus:
macrophages
adenocarcinoma
chemoresistance

Additional relevant MeSH terms:
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Pancreatic Neoplasms
Digestive System Neoplasms
Neoplasms by Site
Neoplasms
Endocrine Gland Neoplasms
Digestive System Diseases
Pancreatic Diseases
Endocrine System Diseases