Optimization of Time-of-Flight Mass Cytometry (CyTOF) Analysis for Evaluation of Immune Changes Following Surgery
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Surgical trauma triggers a massive inflammatory response. Over time, both the innate and adaptive branches of the immune system are affected by surgical trauma. The purpose of this study is to use a single cell flow cytometry approach to characterize the cellular and molecular mechanisms involved in the inflammatory response to surgical trauma.
Numerical (cell frequencies) and functional (phosphorylation of signaling proteins and transcription factors) changes of all circulating immune cells [ Time Frame: Immediately before to surgery, 1 hour after surgery, 24 hours after surgery, and day of hospital discharge ]
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Layout table for eligibility information
Ages Eligible for Study:
18 Years to 90 Years (Adult, Older Adult)
Sexes Eligible for Study:
Accepts Healthy Volunteers:
Generally healthy patients undergoing back surgery
Ages 18 - 90
Men and women
Undergo back surgery
Any systemic disease that might compromise the immune system (e.g. lupus)
Current diagnosis of cancer
Any condition that, in the opinion of the investigator, might compromise the integrity of the study or safety of the participant.