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Myotubular Myopathy Genetic Testing Study

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT01817946
Recruitment Status : Completed
First Posted : March 26, 2013
Last Update Posted : March 7, 2018
Valerion Therapeutics, LLC
Congenital Muscle Disease International Registr
University of Chicago
Boston Children's Hospital
Information provided by (Responsible Party):
Cure CMD

Brief Summary:

Myotubular myopathy (XLMTM) is an X-linked disorder caused by mutations in the myotubularin gene (MTM1). The clinical spectrum is variable and ranges from individuals who require a wheelchair and full time breathing support to those who are able to walk and breathe on their own. Symptoms of myotubular myopathy include long faces, facial weakness with eye muscle weakness, breathing support with a muscle biopsy demonstrating central nucleated fibers. These symptoms may be caused by mutations or changes in the MTM1, BIN1 (bridging integrator 1), DNM2 (dynamin 2) and RYR1 (ryanodine receptor 1) genes. However, the majority are caused by mutations in the MTM1 gene. Some patients with symptoms consistent with myotubular myopathy who initially have negative testing of the MTM1 gene were later found to have a unique type of change in the MTM1 gene. This unique change, called a deletion or duplication, can be found with a different type of genetic test called a CGH (comparative genomic hybridization) array.

Investigators do not know how frequent deletions and duplications are in patients with X-linked myotubular myopathy. Recently, there have been advances in identifying potential treatments for XLMTM. The next step will be to proceed with clinical trials of potential treatments. In order to be ready for clinical trials, it is important that investigators find the specific genetic change that is causing XLMTM in people with this diagnosis. This study will attempt to find changes in the MTM1 gene in individuals who have clinical symptoms consistent with a diagnosis of XLMTM. Participants will be asked to enroll in the CMDIR (Congenital Muscle Disease International Registry), complete a brief clinical survey, provide access to medical records, and provide a saliva or blood sample for genetic testing. Results of genetic testing will be communicated to participants by the physician specified in the consent by the signing person.

Study Hypothesis:

Not all individuals with a clinical diagnosis of XLMTM have access to genetic testing. Investigators know that deletions and duplications of the MTM1 gene can cause XLMTM. Investigators will find more individuals with XLMTM by performing genetic testing of the MTM1 gene, including CGH array for deletions and duplications.

Condition or disease Intervention/treatment
Myotubular Myopathy Other: Genetic Testing

Detailed Description:
  • Prospective participants will complete registration in the CMDIR.
  • The CMDIR genetic curator will review CMDIR data for study eligibility.
  • If eligible, participant will be called and consented to participate in the study.
  • Sequencing can be done with a saliva sample. However, if this method does not detect a mutation or change in the MTM1 gene, a further test, called CGH Array, has to be performed. For the CGH Array test, a saliva sample may deliver a clear result in terms of a deletion/duplication mutation. In the event, CGH Array with the saliva sample does not provide a result, a the blood sample is necessary to repeat CGH Array.
  • The study participant will receive a kit and instructions for saliva specimen collection for genetic testing from the CMDIR in the mail.
  • If a mutation or change in the MTM1 gene could not be found by genetic testing and CGH Array with a saliva sample, the study participant will receive a second kit in the mail from the CMDIR with instructions for a blood draw to be used for CGH Array.
  • The family will coordinate collection of the saliva specimen or a local blood draw, if necessary, and will be responsible for mailing the specimen in a pre-paid parcel to the University of Chicago. There will be a $40 reimbursement for the cost of the blood draw. Mailing the specimen to the testing site is at no cost for the participant. A two-week turn-around from receipt of the kit to sending it to the testing laboratory is requested.
  • Genetic testing will start with standard sequencing of the MTM1 gene, isolated from the saliva specimen, followed by CGH Array if sequencing of the MTM1 gene isolated from the saliva sample or further from a blood sample if a variant consistent with the symptoms could not be detected using the saliva sample.
  • Test results will be 1) reported to a physician specified by the study participant and 2) uploaded into the study participant's profile in the CMDIR and 3) if concurrently enrolled in the Beggs Laboratory or other IRB-approved clinical study, test results will also be made available to that study provided appropriate informed consent has been given.

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Study Type : Observational
Actual Enrollment : 23 participants
Observational Model: Case-Only
Time Perspective: Cross-Sectional
Official Title: Myotubular Myopathy Genetic Testing Study
Actual Study Start Date : March 2013
Actual Primary Completion Date : March 2017
Actual Study Completion Date : March 2017

Group/Cohort Intervention/treatment
Genetic testing
All participants determined eligible for the study will be placed into genetic testing arm.
Other: Genetic Testing
Genetic testing services will be provided by the University of Chicago under the guidance of Dr. Soma Das, PhD. Services will be coordinated by Rachel Alvarez, CMDIR Associate Director. Genetic testing will include standard sequencing of the MTM1 gene followed by CGH array for deletion/duplication in cases where no pathologic variant is identified by standard sequencing.
Other Name: Sequencing

Primary Outcome Measures :
  1. Description of Mutations in the MTM1 Gene by Complete Genetic Sequencing [ Time Frame: 1 year ]
    To confirm presence, locations and frequencies of mutations in the MTM1 gene in study participants presenting with symptoms as listed in the Brief Summary and muscle biopsy and/or family history typical for myotubular myopathy or prior confirmation of a mutation in the MTM1 gene by research sequencing.

Secondary Outcome Measures :
  1. Frequency of Deletion/Duplication Mutations in the MTM1 Gene by CGH Array Testing [ Time Frame: 1 year ]
    To establish the frequency of deletions/duplications in the MTM1 gene for patients who have negative sequencing but have clinical history/muscle biopsy/family history.

  2. To Examine the Relation of the Mutation in the MTM1 Gene to the Phenotype [ Time Frame: 2 years ]
    Investigators will compare mutations characterized by sequencing and CGH array (if necessary) with the following data in the study participant's CMDIR profile: age of onset, ventilatory support needed post-natal and ongoing, maximal motor function achieved, current motor function, need for gastrostomy tube and rate of ER visits and hospitalizations. The individual genotype - phenotype relation will then be compared to that of other study participants. In the event that a genotype-phenotype pattern can be established, this may help predict the course of the disease and prepare for appropriate care.

Biospecimen Retention:   Samples With DNA
saliva, whole blood, or other source

Information from the National Library of Medicine

Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies.

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Ages Eligible for Study:   Child, Adult, Older Adult
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
The study will be composed of individuals registered in the CMDIR who have been clinically diagnosed with XLMTM but do not have an XLMTM diagnosis confirmed by genetic testing.

Inclusion Criteria: Patient eligibility will be determined by the CMDIR genetic curator using the following prioritization protocol.

  1. Males and females in the US and Canada who have a known mutation in the MTM1 gene identified in a research lab and never confirmed in a clinical CLIA (Clinical Laboratory Improvement Amendments) -certified laboratory.
  2. Male and female patients in the US and Canada who meet 2 of 3 of the following criteria: + clinical history, + family history, + centronucleation on muscle biopsy (no signs of nemaline rods or cores). Clinical history includes: post-natal breathing support (not necessarily continued after first month), length above 90% for EGA (estimated gestational age), facial characteristics (narrow facies), facial weakness (ophthalmoplegia, excessive saliva with need for suctioning).
  3. Males and females in the US and Canada who present with XLMTM symptoms and no genetic mutation in the MTM1 gene found with conventional sequencing, requiring CGH array deletion/duplication testing.
  4. Age range: One month - no maximum age.
  5. Individual is registered with the CMDIR.
  6. Written study consent provided by parent/caregiver (affected individual's age less than 18 years or for those individuals greater than 18 years with learning disabilities or inability to physically access consent) or affected individual (age greater than 18 years)

Exclusion Criteria:

1. Carrier testing for asymptomatic mothers.

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT01817946

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United States, California
Congenital Muscle Disease International Registry
Torrance, California, United States, 90502
Sponsors and Collaborators
Cure CMD
Valerion Therapeutics, LLC
Congenital Muscle Disease International Registr
University of Chicago
Boston Children's Hospital
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Principal Investigator: Sabine de Chastonay, PhD CMDIR
Principal Investigator: Elizabeth DeChene, MS, GCG Children's Hospital of Philadelphia
Principal Investigator: Soma Das, PhD University of Chicago Genetic Testing Services Laboratory
Additional Information:
Publications of Results:
Other Publications:

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Responsible Party: Cure CMD Identifier: NCT01817946    
Other Study ID Numbers: CMDIR-001
First Posted: March 26, 2013    Key Record Dates
Last Update Posted: March 7, 2018
Last Verified: March 2018
Keywords provided by Cure CMD:
Congenital Myopathy
Centronuclear Myopathy
Myotubular Myopathy
MTM1 Gene
Genetic testing
Deletion/Duplication Mutation
CGH array
Additional relevant MeSH terms:
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Muscular Diseases
Myopathies, Structural, Congenital
Musculoskeletal Diseases
Neuromuscular Diseases
Nervous System Diseases