An Investigation of Tumor Necrosis Factor (TNF)-Alpha in Asthma Using Biopsy Explants and Primary Bronchial Epithelial Cell Cultures
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|ClinicalTrials.gov Identifier: NCT01161303|
Recruitment Status : Completed
First Posted : July 13, 2010
Last Update Posted : July 13, 2010
We have developed in vitro systems including primary epithelial cell cultures and explant cultures of bronchial tissues to study the interaction between the bronchial mucosa and allergens. This approach involves culturing bronchial biopsies under optimal conditions and stimulate them with allergens thus enabling us to perform a dynamic study without the need of performing several bronchoscopies and will allow the testing of unapproved substances, which could, otherwise, not be delivered, in vivo.
We wish to apply these ex vivo bronchial culture systems to assess cytokine release in moderately severe asthmatics and evaluate the effects of blocking TNF-alpha signalling using anti TNF-alpha monoclonal antibodies.
|Condition or disease|
|Study Type :||Observational|
|Actual Enrollment :||12 participants|
|Official Title:||Study to Investigate the Role of TNF-alpha in Asthma Using Biopsy Explants and Primary Bronchial Epithelial Cell Cultures|
|Study Start Date :||July 2000|
|Actual Primary Completion Date :||June 2003|
|Actual Study Completion Date :||June 2003|
- bronchial brushings and biopsies
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01161303
|Southampton University Hospital Trust|
|Southampton, Hampshire, United Kingdom, SO16 6YD|
|Principal Investigator:||Stephen T Holgate, MD||University of Southampton|