The Influence of Different Concentrations of Glucose in Culture Media on Fertilization and Embryo Quality
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ClinicalTrials.gov Identifier: NCT00606073 |
Recruitment Status : Unknown
Verified January 2008 by Hillel Yaffe Medical Center.
Recruitment status was: Not yet recruiting
First Posted : February 1, 2008
Last Update Posted : February 1, 2008
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Sponsor:
Hillel Yaffe Medical Center
Information provided by:
Hillel Yaffe Medical Center
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Brief Summary:
The aim of this study is to examine whether incubation of human oocytes until pronuclear stage, in different culture media containing high and low concentrations of glucose affect fertilization differently, not only in standard IVF cycles, but also after intracytoplasmatic sperm injection.In addition, we wish to determine whether exposure of oocytes to different concentrations of glucose until pronuclear stage has further effect on embryo quality and development until the blastocyst stage.
Condition or disease | Intervention/treatment | Phase |
---|---|---|
Fertility | Other: Universal IVF Medium + ISM1 Medium | Not Applicable |
Study Type : | Interventional (Clinical Trial) |
Estimated Enrollment : | 100 participants |
Intervention Model: | Parallel Assignment |
Masking: | None (Open Label) |
Primary Purpose: | Treatment |
Official Title: | The Influence of Different Concentrations of Glucose in Culture Media(i.e: ISM1 vs. IVF) on Fertilization and Embryo Quality |
Study Start Date : | February 2008 |
Estimated Primary Completion Date : | August 2009 |
Arm | Intervention/treatment |
---|---|
Active Comparator: I
IVF Procedure-IVF Medium
|
Other: Universal IVF Medium + ISM1 Medium
Sibling oocytes will be equally divided and incubated separately in different 4-well culture dishes containing either IVF medium or ISM1 Medium and while ICSI will be returned to the same culture dish following injection. Fertilization assessment will be performed approximately after 15-20 hours for standard IVF and 12-18 hours post ICSI performance. After assessment, fertilized oocytes from different culture media will be transferred separately into fresh culture dishes containing ISM1 medium only. The zygotes will be incubated until embryo transfer on day 2 or 3. Embryo quality will be assessed according to the morphology of blastomeres as well as it's developmental stage. |
Active Comparator: II
IVF Procedure - ISM1 Medium
|
Other: Universal IVF Medium + ISM1 Medium
Sibling oocytes will be equally divided and incubated separately in different 4-well culture dishes containing either IVF medium or ISM1 Medium and while ICSI will be returned to the same culture dish following injection. Fertilization assessment will be performed approximately after 15-20 hours for standard IVF and 12-18 hours post ICSI performance. After assessment, fertilized oocytes from different culture media will be transferred separately into fresh culture dishes containing ISM1 medium only. The zygotes will be incubated until embryo transfer on day 2 or 3. Embryo quality will be assessed according to the morphology of blastomeres as well as it's developmental stage. |
Active Comparator: III
ICSI Procedure - IVF Medium
|
Other: Universal IVF Medium + ISM1 Medium
Sibling oocytes will be equally divided and incubated separately in different 4-well culture dishes containing either IVF medium or ISM1 Medium and while ICSI will be returned to the same culture dish following injection. Fertilization assessment will be performed approximately after 15-20 hours for standard IVF and 12-18 hours post ICSI performance. After assessment, fertilized oocytes from different culture media will be transferred separately into fresh culture dishes containing ISM1 medium only. The zygotes will be incubated until embryo transfer on day 2 or 3. Embryo quality will be assessed according to the morphology of blastomeres as well as it's developmental stage. |
Active Comparator: IV
ICSI Procedure - ISM1 Medium
|
Other: Universal IVF Medium + ISM1 Medium
Sibling oocytes will be equally divided and incubated separately in different 4-well culture dishes containing either IVF medium or ISM1 Medium and while ICSI will be returned to the same culture dish following injection. Fertilization assessment will be performed approximately after 15-20 hours for standard IVF and 12-18 hours post ICSI performance. After assessment, fertilized oocytes from different culture media will be transferred separately into fresh culture dishes containing ISM1 medium only. The zygotes will be incubated until embryo transfer on day 2 or 3. Embryo quality will be assessed according to the morphology of blastomeres as well as it's developmental stage. |
Primary Outcome Measures :
- Fertilization rate and embryo quality [ Time Frame: 6 days ]
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Ages Eligible for Study: | 18 Years to 50 Years (Adult) |
Sexes Eligible for Study: | All |
Accepts Healthy Volunteers: | Yes |
Criteria
Inclusion Criteria:
- Patients undergoing IVF treatment in our clinic during study period
Exclusion Criteria:
- Females older than 40 years
- Patients with less than 6 retrieved oocytes
- Male patients older than 50 years old
- Patients after testis biopsies
No Contacts or Locations Provided
Responsible Party: | Dr. Medeia Michaeli, Hillel Yaffe Medical Center |
ClinicalTrials.gov Identifier: | NCT00606073 |
Other Study ID Numbers: |
HYMC IRB#31/2007 |
First Posted: | February 1, 2008 Key Record Dates |
Last Update Posted: | February 1, 2008 |
Last Verified: | January 2008 |