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The Cytogenetic Constitution of Embryos Resulting From Immature Oocytes (M1)

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00544531
Recruitment Status : Completed
First Posted : October 16, 2007
Last Update Posted : October 16, 2007
Information provided by:
Assaf-Harofeh Medical Center

Brief Summary:

To examine the chromosomal number content in the blastomeres of embryos achieved from fertilization of immature oocytes at MI stage recruited following COH for IVF.

comparison of the genetic number content of embryos and their quality in terms of cleavage rate and morphological score reached after fertilization of immature oocytes at MI stage whether arrested at the MI stage or extruded the polar body, after in vitro maturation for a variable length of time.

Condition or disease

Detailed Description:
Patients undergoing routine IVF, following informed consent, donated some of their unusable, immature- MI stage oocytes for our research. Following in vitro maturation for up to 2, 4, 8 and 24 hours after harvest, all immature oocytes in these 4 study groups underwent intracytoplasmic donor sperm injection (ICSI), whether they extruded their polar body (MI-PB or rescued MI) or not (arrested MI). Oocyte fertilization rate after 24 hours and embryonic development in terms of cleavage rate and morphology were assessed, for another 48 hours. Then the whole embryo was prepared for molecular cytogenetic evaluation using FISH (Fluorescence in situ hybridization) analysis of chromosomes x, y and 18.

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Study Type : Observational
Actual Enrollment : 23907 participants
Time Perspective: Prospective
Study Start Date : October 2005
Actual Study Completion Date : March 2007

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Infertility

Information from the National Library of Medicine

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Ages Eligible for Study:   25 Years to 35 Years   (Adult)
Sexes Eligible for Study:   Female
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • Patients with presence of immature oocytes after ART stimulation

Exclusion Criteria:

  • Patients with less than total 7 oocytes

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00544531

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Infertility unit,Assaf-Harofeh Medical Center
Zrifin, Israel, 70300
Sponsors and Collaborators
Assaf-Harofeh Medical Center
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Principal Investigator: Raphael Ron-el, professor Assaf-Harofeh Medical Center
Layout table for additonal information Identifier: NCT00544531    
Other Study ID Numbers: 76/05
First Posted: October 16, 2007    Key Record Dates
Last Update Posted: October 16, 2007
Last Verified: September 2005
Keywords provided by Assaf-Harofeh Medical Center:
MI oocytes, FISH,chromosomal aberrations
Additional relevant MeSH terms:
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Genital Diseases, Male
Genital Diseases, Female