Activation and Recruitment of GIL During Hp Infection

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00275886
Recruitment Status : Unknown
Verified December 2005 by National Taiwan University Hospital.
Recruitment status was:  Recruiting
First Posted : January 12, 2006
Last Update Posted : January 12, 2006
Information provided by:
National Taiwan University Hospital

Brief Summary:
Helicobacter pylori is a major human pathogen that infects over half of the world population. Infection initiates a series of changes in the gastric mucosa, beginning with gastritis and leading in some patients to peptic ulcer disease, mucosa-associated lymphomas, and gastric adenocarcinoma. It is believed that host factors, in particular, the T cell-mediated immune responses may play an important role in the pathogenesis of diseases induced by H. pylori infection. Recent results revealed that there were higher IFN-γ secreting cells in gastric infiltrating T cells isolated from H. pylori infected patients than in uninfected patients, suggesting that the TH1 response and degree of IFN-γ production is associated with disease severity. Meanwhile, recent studies have shown that apoptosis of the gastric epithelium is increased during infection and this response is associated with an expansion of gastric T-helper type 1 (Th1) cells. In this project, we are trying to further investigate role of host T cell mediated immune response in pathogenesis of Helicobacter infection by characterization of the expression of chemokine receptors on gastric infiltrating lymphocytes. We are going to investigate the mechanisms involving in chemokine/chemokine receptor interaction in recruitment of gastric infiltrating lymphocytes and pathogenesis of gastric mucosa damage in Helicobacter infection. This study will be helpful for understanding the mechanisms of activation and recruitment of gastric-infiltrating lymphocytes during gastric inflammation.

Condition or disease
Helicobacter Gastritis Dyspepsia

Detailed Description:

Specific Aim #1: Characterization of the gastric infiltrating T cells from H. pylori gastritis patients A. Patients H. pylori infected gastritis and asymptomatic patients are selected from National Taiwan University hospital. The gastric biopsy specimen are taken from endoscopic biopsy in routine health check up or panendoscopic examination for the despeptic patients. HP negative gastritis patients will also be studied for negative control.

B. Immunohistochemical identification of T cells and expression of chemokine receptors.

Specific Aim #2 . Isolation and characterization of chemokines and chemokine receptors of the infiltrating lymphocytes in H. pylori-associated gastritis tissue

A. Isolation of gastric infiltrating lymphocyte B. Identification of the expression of chemokine receptor in gastric infiltrating lymphocytes by flow cytometry C. Detection of expression of chemokines in gastric tissues

Study Type : Observational
Enrollment : 30 participants
Observational Model: Case Control
Primary Purpose: Screening
Time Perspective: Cross-Sectional
Time Perspective: Retrospective
Official Title: Activation and Recruitment of Gastric Infiltrating Lymphocytes During H. Pylori Infection
Study Start Date : January 2006
Study Completion Date : December 2008

Information from the National Library of Medicine

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Ages Eligible for Study:   20 Years to 60 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No

Inclusion Criteria:

  • patients receiveing endoscopy examination due to dyspepsia

Exclusion Criteria:

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00275886

Contact: Ping-Ning Hsu, MD, PhD 886-2-23123456 ext 8635

National Taiwan University Hospital Recruiting
Taipei, Taiwan
Contact: Ping-Ning Hsu, MD, PhD    +886-223123456 ext 8635   
Principal Investigator: Ping-Ning Hsu, MD, PhD         
Sponsors and Collaborators
National Taiwan University Hospital
Principal Investigator: Ping-Ning Hsu, MD, PhD Department of Immunology Identifier: NCT00275886     History of Changes
Other Study ID Numbers: 9461701221
First Posted: January 12, 2006    Key Record Dates
Last Update Posted: January 12, 2006
Last Verified: December 2005

Additional relevant MeSH terms:
Signs and Symptoms, Digestive
Signs and Symptoms
Gastrointestinal Diseases
Digestive System Diseases
Stomach Diseases