• INCLUSION CRITERIA:

Patient must meet at least one of these criteria:

Have suspected or known disorder of the immune system or malignancy

Be a known or potential carrier of genetically determined autoimmune disorder or immunodeficiency disease.

Specific i disorders may include but are not limited to: X-linked (severe combined immunodeficiency) SCID (c gamma deficiency), Autosomal recessive SCID, X-linked CD40 ligand deficiency, Common variable immunodeficiency, Ataxia-telangiectasia, Wiskott Aldrich syndrome, DiGeorge syndrome and Infection with HTLV-1

Age of birth and above for patients with suspected or known disorders of the immune system.

Patient (or parent/guardian of a minor child) must be able to understand and sign informed consent.

Hematocrit greater than 28%, and platelet count greater than 50,000 necessary for apheresis.

Subjects for whom apheresis is desired but whose counts are lower than those above must be evaluated and approved by a Department of Transfusion Medicine consult physician.

Weight greater than 25 kg is necessary for apheresis.

EXCLUSION CRITERIA:

Overall Exclusion Criteria:

Pregnant or breast feeding women will not be eligible for any aspect of this protocol except phlebotomy

Children less than 18 years old are not eligible for lymph node or bone marrow biopsy

Exclusion Criteria for skin/parenteral antigen tests:

Any history of severe reaction or allergy to a particular skin test antigen or other ingredients in the formulation (e.g. Thimerosal, eggs or avian protein) will exclude a subject from receiving that particular skin test.

Children under the age of 2 years are not eligible to receive the Pneumococcal polyvalent vaccine.

Subjects under the age of 18 years are not eligible to receive the Candida or mumps skin test antigens.

Exclusion Criteria for Apheresis Alone:

Any diagnosed medical condition which may be worsened by the apheresis procedure. Specifically the patient should not have any of the following:

1. Congestive Heart Failure
2. History of angina
3. Severe hypotension (at the discretion of the patient's physician, the apheresis staff and the attending physician from the Department of Transfusion Medicine (DTM) per DTM Standard Operating Policies.)
4. Poorly controlled hypertension (average baseline blood pressure greater than 160/90)
5. History of a coagulation protein disorder.

Pediatric normal volunteers (less than 18 years) will not undergo apheresis.