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Sleep Deprivation's Regulation of Immune System Function and Behavior (SS)

This study is ongoing, but not recruiting participants.
Sponsor:
Information provided by (Responsible Party):
Uppsala University
ClinicalTrials.gov Identifier:
NCT01730742
First received: October 11, 2012
Last updated: November 15, 2012
Last verified: November 2012
History of Changes

October 11, 2012
November 15, 2012
February 2012
March 2013   (final data collection date for primary outcome measure)
Neutrophil phagocytotic function production [ Time Frame: Change in 4 neutrophil phagocytotic function from baseline to 36hours later (after the nighttime intervention) ] [ Designated as safety issue: No ]

This project is designed to test the inflammatory capacity of circulating neutrophils. It also aims to investigate if any changes in neutrophil efficacy are governed by alterations in DNA methylation.

Measured during 36 hours (at 1930 day 1, 0730 and 1930 day 2, 0730 day 3)
Same as current
Complete list of historical versions of study NCT01730742 on ClinicalTrials.gov Archive Site
  • Portion Size Task [ Time Frame: Change in selected portion size from baseline to 1 hr after consuming a caloric preload ] [ Designated as safety issue: No ]
    Participants will be evaluated on their tendency to choose larger or smaller portions of a variety of meal items on a computer screen. This will be conducted both following sleep deprivation and sleep, and changes over the hour will be compared between these conditions.
  • Neuro-economics task [ Time Frame: Change in purchasing behaviour 1 hr after consuming a caloric preload ] [ Designated as safety issue: No ]
    Participants will be evaluated on their purchasing behavior with regards to high-calorie and low-calorie food items in a mock supermarket scenario following sleep and sleep deprivation.
  • Circulating hormone levels [ Time Frame: Change in circulating hormone levels from baseline (ie. 1930 - before sleep intervention) to 12hr later (0730 after the nighttime intervention) ] [ Designated as safety issue: No ]
    Participants will have their circulating hormone levels taken and analyzed, including ghrelin and oxytocin, to determine if sleep deprivation alters hormone levels related primarily to obesity or weight gain
Same as current
Not Provided
Not Provided
 
Sleep Deprivation's Regulation of Immune System Function and Behavior
The Role of Sleep Deprivation in the Regulation of Immune System, Neuroendocrine Responses, and Behavioral Measures.

The study proposes to investigate whether sleep deprivation will affect a variety of measures, including hormones, immune system functioning, and behaviors related to food intake and hunger. It is predicted that sleep deprivation will affect circulating neutrophil activity, and do so via affects on DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and down-regulate circulating oxytocin. Finally, it is predicted that sleep deprivation will increase participants' tendencies to pick larger portions of food, and also increase their tendency to purchase foods that are more caloric in a mock supermarket scenario.
Not Provided
Interventional
Not Provided
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Open Label
Primary Purpose: Basic Science
  • Sleep Deprivation
  • Sleep
  • Behavioral: Neuroeconomics task
    After a night of wakefulness, participants performed the neuroeconomics task (shopping food items in a mock supermarket scenario).
  • Behavioral: Portion Size Task
    Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume
  • Procedure: Blood sample
    After a night of wakefulness, a blood sample was taken to assess the level and efficacy of circulating neutrophils, as well as to assay blood serum and plasma for the presence of hormones involved in hunger such as oxytocin and ghrelin
  • Experimental: Sleep deprivation
    Total sleep deprivation: participants were required to stay up for the entire night before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed
    Interventions:
    • Behavioral: Neuroeconomics task
    • Behavioral: Portion Size Task
    • Procedure: Blood sample
  • Experimental: Sleep
    Sleep: participants had an 8-h sleep opportunity before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed
    Interventions:
    • Behavioral: Neuroeconomics task
    • Behavioral: Portion Size Task
    • Procedure: Blood sample
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Active, not recruiting
18
March 2013
March 2013   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • Male
  • Age 18-35y
  • Healthy (self-reported) and not on medication
  • Non-smoking
  • Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported)

Exclusion Criteria:

  • Major illness
  • Taking any serious medications
  • Any sleep conditions (e.g. irregular bedtimes, sleep complaints)
  • Any dietary issues with the food items provided
  • A history of endocrine or psychiatric disorders
Male
Not Provided
Yes
Contact information is only displayed when the study is recruiting subjects
Sweden
 
NCT01730742
192837465
No
Uppsala University
Uppsala University
Not Provided
Principal Investigator: Christian Benedict, PhD dept. of Neuroscience, Uppsala University
Uppsala University
November 2012

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP