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Effects of Acute Exercise on Acetylcarnitine Concentration in Endurance Trained and Untrained Subjects

The recruitment status of this study is unknown because the information has not been verified recently.
Verified July 2012 by Maastricht University Medical Center.
Recruitment status was  Recruiting
Sponsor:
Information provided by (Responsible Party):
Maastricht University Medical Center
ClinicalTrials.gov Identifier:
NCT01553968
First received: March 7, 2012
Last updated: July 26, 2012
Last verified: July 2012

March 7, 2012
July 26, 2012
April 2012
September 2012   (final data collection date for primary outcome measure)
Exercise-induced changes in acetylcarnitine concentrations and dynamics of acetylcarnitine restoration after exercise [ Time Frame: During 30 minutes prior to the exercise and for 30 minutes after exercise ] [ Designated as safety issue: No ]
Acetylcarnitine concentration measured with Proton Magnetic Resonance Spectroscopy (1H-MRS)
Exercise-induced changes in acetylcarnitine concentrations and dynamics of acetylcarnitine restoration after exercise [ Time Frame: During 30 minutes prior to the exercise and for 30 minutes after exercise ] [ Designated as safety issue: No ]
Acetylcarnitine concetration measured with Proton Magnetic Resonance Spectroscopy (1H-MRS)
Complete list of historical versions of study NCT01553968 on ClinicalTrials.gov Archive Site
  • Substrate oxidation [ Time Frame: Measured during the 30 minutes of exercise ] [ Designated as safety issue: No ]
    Measured with indirect calorimetry
  • Blood plasma free fatty acids [ Time Frame: At the start and at the end of 30 minutes of exercise ] [ Designated as safety issue: No ]
    Blood sample of 10 mL
  • Blood plasma triglycerides [ Time Frame: At the start and at the end of 30 minutes of exercise ] [ Designated as safety issue: No ]
    Blood sample of 10 mL
  • Blood plasma glucose [ Time Frame: At the start and at the end of 30 minutes of exercise ] [ Designated as safety issue: No ]
    Blood sample of 10 mL
Same as current
Not Provided
Not Provided
 
Effects of Acute Exercise on Acetylcarnitine Concentration in Endurance Trained and Untrained Subjects
Effects of Acute Exercise on Acetylcarnitine Concentration in Endurance Trained and Untrained Subjects

It has been suggested that imbalance between TCA-cycle flux and β-oxidation may underlie insulin resistance, a predisposing factor for the development of type 2 diabetes mellitus. Acetylcarnitine concentration is suggested to be a marker of such imbalance. It is expected that when TCA-cycle capacity is high (a high oxidative capacity), less acetylcarnitine will accumulate, because of an improved balance between supply and demand of lipids.

The major research objective is to examine if acute exercise results in a more pronounced increase in acetylcarnitine concentration in sedentary subjects compared to endurance-trained subjects and if the exercise-induced increase in acetylcarnitine is restored more quickly in endurance-trained subjects when compared to sedentary subjects.

The investigators hypothesize that the increase in acetylcarnitine levels will be lower in trained subjects when compared to sedentary subjects, due to a better balance between lipid supply and utilization by the TCA-cycle. Furthermore it is expected that acetylcarnitine concentrations will be restored faster in these trained subjects, because of a tighter regulation of influx of fatty acids.

To test this hypothesis the investigators want to compare the acetylcarnitine response to exercise in a group of sedentary subjects and a group of endurance trained subjects. This response will be measured for 30 minutes after exercise with the use of 1H-MRS.

Not Provided
Interventional
Not Provided
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Open Label
Primary Purpose: Basic Science
Diabetes Mellitus, Type 2
Other: Cycling
30 minutes of cycling at 50% of predetermined maximal performance
  • Endurance Trained Subjects
    Intervention: Other: Cycling
  • Untrained Subjects
    Intervention: Other: Cycling
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruiting
13
December 2012
September 2012   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • Normal weight (BMI 18-25 kg/m2)
  • Healthy
  • Stable dietary habits
  • No use of medication
  • VO2-max for trained subjects above 50 mL/min/kg
  • VO2-max for untrained subjects below 40 mL/min/kg

Exclusion Criteria:

  • Any medical condition requiring treatment and/or medication use OR diminishing exercise tolerance
  • Alcohol consumption of more than 20 g per day (± 2 units)
  • Unstable body weight (weight gain or loss > 3 kg in the past three months)
  • Participation in another biomedical study within 1 month prior to the screening visit
  • Contraindications for MRI scan:

    • Central nervous system aneurysm clips
    • Implanted neural stimulator
    • Implanted cardiac pacemaker of defibrillator
    • Cochlear implant
    • Iron- containing corpora aliena in the eye or brain
    • Hearing aids and artificial (heart) valves which is contraindicated for MRS
  • Subjects, who do not want to be informed about unexpected medical findings cannot participate in the study.
Both
18 Years to 40 Years
Yes
Contact: Lucas Lindeboom, MSc. +31433882937 lucas.lindeboom@maastrichtuniversity.nl
Netherlands
 
NCT01553968
11-2-069
No
Maastricht University Medical Center
Maastricht University Medical Center
Not Provided
Principal Investigator: Vera B. Schrauwen-Hinderling, PhD. Maastricht University Medical Center
Maastricht University Medical Center
July 2012

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP