Effects of Oral Probiotic Supplementation on Group B Strep Rectovaginal Colonization in Pregnancy

The investigators wish to determine if oral probiotic supplementation during the second half of pregnancy decreases maternal GBS recto-vaginal colonization at 35-37 weeks' gestational age, thereby decreasing need for maternal antibiotic administration at time of labor. The importance of this study is that it may offer a safer alternative to antibiotic treatment of GBS colonized pregnant women.

1. Screening: All pregnant women delivering at LPCH or receiving obstetric care at any of the satellite research sites in Santa Cruz will be offered enrollment in the study prior to 28 weeks.
2. Women will continue regular and routine obstetric care and clinic visits.
3. Placebo vs probiotic daily regimen: We plan to begin administration of product and placebo at 20 weeks gestation, and no later than 28 weeks gestation until delivery. Once a women is enrolled in the study, she will be randomized to either the placebo or the probiotic group.
4. At the time of randomization, the patient will receive her month supply of 30 capsules; The allocation arm will be double-blinded.
5. We will schedule the women for routine monthly obstetric visits (more often if clinically required) during which time they will also meet with one of the investigators. The investigator at each monthly visit will provide an additional monthly allotment of 30 capsules. The capsule bottle from the previous cycle will be collected and dated if there are capsules remaining in the bottle. Remaining capsules will be counted and refrigerated for future use.
6. We will collect history data including safety data per the questionnaire and will document compliance with the study.
7. GBS recto-vaginal screening: We will enroll the women in the study and we will perform the standard GBS colonization screening (using standard GBS rectovaginal cultures) at 36 weeks.
8. Additionally, subjects may opt to have serial vaginal swabs collected to assess potential beneficial effects of probiotics on the vaginal microbiota and BV status. Vaginal swabs will be collected (either by study personnel or self-collected by the study participant). Swabs will be inserted 1-2 inches into the vaginal introitus and spun for 20 seconds and then withdrawn. Swabs will be collected at the following time points: prior to probiotic/placebo initiation, every 1-4 weeks from time of enrollment to time of delivery, and postpartum serially up to 12 months. These swabs will be stored at -20 degrees Celsius or colder for additional microbiologic analyses.
9. Additionally, placental tissue may be collected at time of delivery for possible future microbiome and/or other analyses.
10. Women who suffer a premature rupture of the membranes, deliver before 36 weeks gestation, or go into labor before the GBS culture result is available, will receive the standard GBS antibiotic prophylaxis.
11. Labor: The patient will receive standard delivery and newborn care. Patients with a positive GBS culture will be treated with standard antibiotics in labor.
12. Postpartum and neonatal care: The patient will receive routine postpartum care per the obstetric team. Data regarding her postpartum course and neonatal outcomes will be collected.

• Infection
• Pregnancy

• Dietary Supplement: Placebo
  One placebo capsule daily.
  Other Name: Sugar pill
• Dietary Supplement: Probiotic dietary supplement
  Dietary Supplement: Lactobacillus rhamnosus GR-1, Lactobacillus reuteri RC -14
  Other Names:

  • Other Names:
  • Jarrow's fem-dophilus

• Active Comparator: Probiotic dietary supplement
  Probiotic dietary supplement one capsule once per day until delivery.
  Intervention: Dietary Supplement: Probiotic dietary supplement
• Placebo Comparator: Placebo
  Placebo capsule, one daily until delivery.
  Intervention: Dietary Supplement: Placebo

Inclusion Criteria:

1. Pregnant women between 20-28 weeks gestation.
2. 18 years of age or older.
3. Singleton gestation.

Exclusion Criteria:

1. Preexisting morbidity: Immunocompromised status (HIV +; malignancy; history of organ transplant; chronic steroid therapy; autoimmune disease requiring treatment during pregnancy, and other immunocompromised states); Type 1 diabetes and type 2 diabetes;congenital cardiac disease and cardiac valvular disease requiring antibiotic prophylaxis during procedure/labor; pulmonary disease (except mild asthma); renal disease; chronic hepatic disease (Hepatitis B, C); inflammatory bowel disease (Crohn's disease or ulcerative colitis); stomach or duodenal ulcer; bowel resection, gastric bypass, and chronic indwelling venous, bladder, or gastric catheter.
2. Multi-fetal gestation.
3. Use of probiotics preparations in the 3 months prior to beginning of the study treatment or use of any additional probiotics preparations (other than study treatment) at any time during the study period (including over the counter food supplements such as Activia, BioK, other oral or vaginal probiotics products (BUT not including other common forms of yogurt).
4. Chronic (daily) use of broad spectrum antibiotics.
5. Prolonged antibiotic use (> 7 days) in the 4 weeks prior to GBS culture screening.
6. History of infant with GBS sepsis.
7. IUGR, Fetal Anomalies-major diagnosed at time of second trimester anatomy ultrasound
8. Anticipated delivery <35 wks for maternal/fetal indication
9. Placenta previa or accreta (with anticipated delivery prior to 35 weeks)