Chronic Presence of Epstein Barr Virus in Sulcular and Junctional Epithelial Tissue From Gingiva is Associated With Severe Periodontitis

• Presence/ absence of latency and/or lytic proteins (by immunofluorescence and in situ hybridization [ Time Frame: at time = 0 (baseline) ] [ Designated as safety issue: No ]
• - Epithelial tissue infected (by Immunofluorescence and In situ Hybridization) : detection of oral gingival epithelium, sulcular epithelium, junctional epithelium. [ Time Frame: at time = 0 (baseline) ] [ Designated as safety issue: No ]

• Presence/ absence of latency and/or lytic proteins (by immunofluorescence and in situ hybridization [ Time Frame: at time = 0 ] [ Designated as safety issue: No ]
• - Epithelial tissue infected (by Immunofluorescence and In situ Hybridization) : detection of oral gingival epithelium, sulcular epithelium, junctional epithelium. [ Time Frame: at time = 0 ] [ Designated as safety issue: No ]

The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.

• Parodontitis Aggressive
• Parodontitis Chronic

• Procedure: Biopsy
  dental extraction and gingival epithelial biopsy
• Procedure: biopsy
  dental extraction and gingival epithelial biopsy

• Active Comparator: chronic periodontitis
  Intervention: Procedure: Biopsy
• Active Comparator: agressive periodontitis
  Intervention: Procedure: biopsy
• Active Comparator: healthy patients
  Intervention: Procedure: Biopsy

Inclusion Criteria:

• Men exclusively
• 12 years minimum
• 2 patients affected by chronic periodontitis
• 2 patients affected by aggressive periodontitis
• 2 healthy patients (orthodontics extraction)
• Subject who have read and understood the information note relative under investigation and who have signed the informed consent
• Subject whose legal representative signed the parental consent (if minor patient)

Exclusion Criteria:

• Patient with contre-indication to dental extraction
• Patient witn serious blood disease
• Patient who exhibits systemic condition incompatible with the realization of the study
• Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants
• Patient who presents za buccal carcinoma treated with radiotherapy
• Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy
• Patient who presents a linguistic or psychic incapacity to understand information
• Female patient