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Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome (IBS)

The recruitment status of this study is unknown because the information has not been verified recently.
Verified December 2006 by Chinese PLA General Hospital.
Recruitment status was  Active, not recruiting
Sponsor:
Information provided by:
Chinese PLA General Hospital
ClinicalTrials.gov Identifier:
NCT01028898
First received: December 8, 2009
Last updated: February 10, 2011
Last verified: December 2006
History of Changes

December 8, 2009
February 10, 2011
January 2006
August 2011   (final data collection date for primary outcome measure)
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Complete list of historical versions of study NCT01028898 on ClinicalTrials.gov Archive Site
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Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome
Screening and Identification of Abnormal Expression Proteins and miRNA From Colonic Mucosa of Patients With Irritable Bowel Syndrome.

In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.
Observational
Observational Model: Case Control
Time Perspective: Prospective
Not Provided
Retention:   Samples With DNA
Description:

The investigators enrolled IBS-D and IBS-C patients on the Rome III criteria and healthy volunteers as normal control group. Biopsies were taken from the cecum and sigmoid colon under direct vision. Five pieces were taken separately at each site. Four pieces were processed by cold saline water and frozen in liquid nitrogen immediately while the other one was fixed in formalin and then embedded in paraffin three days later.
Probability Sample

IBS patients (IBS-D and IBS-C)and healthy control
  • Irritable Bowel Syndrome
  • Digestive System Diseases
  • Gastrointestinal Diseases
  • Intestinal Diseases
  • Colonic Diseases
  • Colonic Diseases, Functional
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*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Active, not recruiting
180
December 2012
August 2011   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • Patients with symptoms meeting Rome III criteria for diagnosis of IBS-C and IBS-D

Exclusion Criteria:

  • Patients with organic illnesses or a history of major abdominal surgery
  • Major psychotic episode, mental retardation or dementia
  • Pregnant women and lactational women
  • Postinfectious IBS, PI-IBS
Both
18 Years to 60 Years
Yes
Contact information is only displayed when the study is recruiting subjects
Not Provided
 
NCT01028898
IBS and metabolism
Yes
YANG Yun-sheng, ChinaPLAGH
Chinese PLA General Hospital
Not Provided
Study Chair: YANG Yun-sheng, MD Chinese PLA General Hospital
Principal Investigator: ZHANG Chun-yan, doctor Chinese PLA General Hospital
Principal Investigator: WANG Weifeng, doctor Chinese PLA General Hospital
Principal Investigator: YAO XIN, DOCTOR Chinese PLA General Hospital
Principal Investigator: PENG LIHUA, DOCTOR Chinese PLA General Hospital
Principal Investigator: YU YUANZI, DOCTOR Chinese PLA General Hospital
Chinese PLA General Hospital
December 2006

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP