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Corneal Epithelial Wound Healing Difference Between Human Autoserum and Cord Blood Serum

This study has been completed.
Sponsor:
Information provided by:
National Taiwan University Hospital
ClinicalTrials.gov Identifier:
NCT00681642
First received: May 19, 2008
Last updated: January 6, 2011
Last verified: December 2010

May 19, 2008
January 6, 2011
March 2007
March 2008   (final data collection date for primary outcome measure)
Cell proliferation [ Time Frame: 1 week ] [ Designated as safety issue: No ]
Same as current
Complete list of historical versions of study NCT00681642 on ClinicalTrials.gov Archive Site
Cell migration [ Time Frame: 1 week ] [ Designated as safety issue: No ]
Same as current
Not Provided
Not Provided
 
Corneal Epithelial Wound Healing Difference Between Human Autoserum and Cord Blood Serum
Phase 1 Study of Comparison of the Effects on Promoting Corneal Epithelial Wound Healing Between Human Autoserum and Cord Blood Serum—in Vitro Cell Culture Experiment

Human serum eye drops have been successfully used in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.

Not Provided
Observational
Observational Model: Case Control
Time Perspective: Prospective
Not Provided
Not Provided
Non-Probability Sample

Healthy pregnant women underwent smooth cesarean section during labor Healthy blood donor

Corneal Epithelial Wound Healing
Not Provided
  • 1
    Corneal epithelial tissue with wound cultured in human autoserum
  • 2
    Corneal epithelial tissue with wound cultured in umbilical cord serum

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Completed
7
March 2008
March 2008   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • Healthy pregnant women
  • Cesarean section for labor
  • Complete placenta with umbilical cord retained
  • Healthy individual

Exclusion Criteria:

  • The retained placenta and umbilical cord were not complete
  • Individual with anemia or other hematologic disorder unsuitable for blood donation
Both
Not Provided
Yes
Contact information is only displayed when the study is recruiting subjects
Taiwan
 
NCT00681642
200702037R
No
Wei-Li Chen, MD, PhD, Assistant Professor, National Taiwan University Hospital, department of Ophthalmology
National Taiwan University Hospital
Not Provided
Principal Investigator: Wei-Li Chen, MD, PhD National Taiwan University Hospital, department of Ophthalmology
National Taiwan University Hospital
December 2010

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP