Comparison of the Effects on Promoting Corneal Epithelial Wound Healing Between Human Auto-Serum and Cord Blood Serum

This study has been completed.
Sponsor:
Information provided by:
National Taiwan University Hospital
ClinicalTrials.gov Identifier:
NCT00598299
First received: January 10, 2008
Last updated: November 2, 2008
Last verified: October 2008

January 10, 2008
November 2, 2008
April 2007
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Complete list of historical versions of study NCT00598299 on ClinicalTrials.gov Archive Site
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Comparison of the Effects on Promoting Corneal Epithelial Wound Healing Between Human Auto-Serum and Cord Blood Serum
Department of Ophthalmology, National Taiwan University Hospital

Human serum eye drops have been successfully used in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.

Human serum eye drops have been successfully used clinically in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.

Observational
Observational Model: Case Control
Time Perspective: Prospective
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Non-Probability Sample

heahty adult volunteers and donated cord blood

Eye Injuries
  • Procedure: venous puncture
    venous puncture
  • Other: obtain serum from perripheral blood or cord blood
    obtain serum from perripheral blood or cord blood
  • A
    Healthy adult serum
    Interventions:
    • Procedure: venous puncture
    • Other: obtain serum from perripheral blood or cord blood
  • B
    cord blood serum
    Interventions:
    • Procedure: venous puncture
    • Other: obtain serum from perripheral blood or cord blood
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Completed
100
April 2008
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Inclusion Criteria:

healthy volunteers

Exclusion Criteria:

unhealthy volunteers

Both
18 Years to 60 Years
Yes
Contact information is only displayed when the study is recruiting subjects
Taiwan
 
NCT00598299
20072037R
Yes
Wei-Li Chen, Department of Ophthalmology, National Taiwan University Hospital
National Taiwan University Hospital
Not Provided
Study Chair: Fung-Rong Hu, MD National Taiwan University Hospital
National Taiwan University Hospital
October 2008

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP