Nasal Epithelium Gene Expression Profiling in Child Respiratory Allergic Disease

This study has been completed.
Sponsor:
Collaborator:
ADPHUN, FRM
Information provided by:
Centre Hospitalier Universitaire de Nice
ClinicalTrials.gov Identifier:
NCT00569361
First received: December 6, 2007
Last updated: June 8, 2009
Last verified: June 2009

December 6, 2007
June 8, 2009
November 2004
Not Provided
Transcriptome analysis: genes statistically modulated between groups, predictive biomarkers allowing correct classification of groups [ Time Frame: At the sample time ] [ Designated as safety issue: No ]
Same as current
Complete list of historical versions of study NCT00569361 on ClinicalTrials.gov Archive Site
Validation with other techniques: Q-PCR [ Time Frame: At the sample time ] [ Designated as safety issue: No ]
Same as current
Not Provided
Not Provided
 
Nasal Epithelium Gene Expression Profiling in Child Respiratory Allergic Disease
Nasal Epithelium Gene Expression Profiling in Child Respiratory Allergic

Using a human pangenomic microarray, the researchers established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct groups:

  1. allergic rhinitis to dust mite (AR) isolated (n=12),
  2. AR associated with bronchial hyperreactivity (n=12),
  3. AR associated with asthma (n=14),
  4. control (n=14).

Context: Asthma is the most frequent chronic disease in childhood. Allergic rhinitis has been described as a risk factor to develop asthma. The objective of the study was to evaluate the contribution of the respiratory epithelium to development of allergic rhinitis and asthma and to identify the molecular mechanisms driving rhinitis toward asthma.

Methods: Using a human pangenomic microarray, we established expression profiles of nasal epithelial cells, collected by brushing of patients belonging to one of four distinct children 2 to 18 years of age groups:

  1. allergic rhinitis to dust mite (AR) isolated (n=12),
  2. AR associated with bronchial hyperreactivity (n=12),
  3. AR associated with asthma (n=14),
  4. control (n=14).
Interventional
Not Provided
Endpoint Classification: Efficacy Study
Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Screening
Allergic Rhinitis
Procedure: Collection of nasal epithelial cells
Collection of nasal epithelial cells by brushing
A
Collection of nasal epithelial cells by brushing
Intervention: Procedure: Collection of nasal epithelial cells
Giovannini-Chami L, Marcet B, Moreilhon C, Chevalier B, Illie MI, Lebrigand K, Robbe-Sermesant K, Bourrier T, Michiels JF, Mari B, Crénesse D, Hofman P, de Blic J, Castillo L, Albertini M, Barbry P. Distinct epithelial gene expression phenotypes in childhood respiratory allergy. Eur Respir J. 2012 May;39(5):1197-205. doi: 10.1183/09031936.00070511. Epub 2011 Oct 17.

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Completed
80
October 2005
Not Provided

Inclusion Criteria:

Patients:

  • Patients between 6 and 17 years old
  • Patients with allergic rhinitis

Witness:

  • Patients between 6 and 17 years old
  • Patients without allergic rhinitis and asthma

Exclusion Criteria:

  • Rhino-bronchitis infection dated from less than 15 days
  • Patients younger than 6 or older than 17 years
Both
6 Years to 17 Years
No
Contact information is only displayed when the study is recruiting subjects
France
 
NCT00569361
CIR 2005
No
Département de la Recherche Clinique et de l'Innovation, CHU de Nice
Centre Hospitalier Universitaire de Nice
ADPHUN, FRM
Principal Investigator: Marc Albertini, Professor Department of Pediatrics of Nice University Hospital
Centre Hospitalier Universitaire de Nice
June 2009

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP