Laboratory-Treated T Cells and Aldesleukin After Cyclophosphamide in Treating Patients With Stage IV Melanoma

This study has been completed.
Sponsor:
Information provided by:
Fred Hutchinson Cancer Research Center
ClinicalTrials.gov Identifier:
NCT00553306
First received: November 2, 2007
Last updated: April 21, 2011
Last verified: April 2011

November 2, 2007
April 21, 2011
September 2007
August 2010   (final data collection date for primary outcome measure)
  • Safety and toxicity as assessed by NCI CTC version 3.0 [ Time Frame: 8 weeks post treatment ] [ Designated as safety issue: Yes ]
  • Antitumor effects of CD4+ and CD8+ antigen-specific T-cells [ Time Frame: 8 weeks post treatment ] [ Designated as safety issue: No ]
  • Duration of in vivo persistence of adoptively transferred CD8+ antigen-specific T cell clones in the presence or absence of transferred CD4+ T cells [ Time Frame: 8 weeks post treatment ] [ Designated as safety issue: No ]
  • Toxicity
  • Antitumor efficacy
  • Duration of in vivo persistence of adoptively transferred CD8+ antigen-specific T cell clones in the presence or absence of transferred CD4+ T cells
  • Safety
  • Influence of low-dose aldesleukin in enhancing the in vivo persistence of transferred CD4 and CD8 T cells
Complete list of historical versions of study NCT00553306 on ClinicalTrials.gov Archive Site
In vivo antitumor efficacy of the infused autologous antigen-specific CD4+ T cells [ Time Frame: 8 weeks post treatment ] [ Designated as safety issue: No ]
Not Provided
Not Provided
Not Provided
 
Laboratory-Treated T Cells and Aldesleukin After Cyclophosphamide in Treating Patients With Stage IV Melanoma
Phase I Study To Evaluate Cellular Adoptive Immunotherapy Using Autologous CD8+ Antigen-Specific T Cell Clones Following Cyclophosphamide Conditioning For Patients With Metastatic Melanoma

RATIONALE: Laboratory-treated T cells may be able to kill tumor cells when they are put back into the body. Aldesleukin and cyclophosphamide may stimulate the immune system in different ways and stop tumor cells from growing. Giving laboratory-treated T cells together with aldesleukin after cyclophosphamide may be an effective treatment for melanoma.

PURPOSE: This phase I/II trial is studying the side effects of giving laboratory-treated T cells together with aldesleukin after cyclophosphamide and to see how well they work in treating patients with stage IV melanoma.

PRIMARY OBJECTIVES:

I. To assess the safety and toxicity of cellular adoptive immunotherapy in melanoma patients using autologous CD4+ and CD8+ antigen-specific T cell clones.

II. To evaluate the antitumor effects of CD4+ and CD8+ antigen-specific T cells in patients with metastatic melanoma.

III. To determine the duration of in vivo persistence of adoptively transferred CD8+ antigen-specific T cell clones in the presence or absence of transferred CD4+ T cells.

SECONDARY OBJECTIVES:

I. To assess the in vivo antitumor efficacy of the infused autologous antigen-specific CD4+ T cells.

OUTLINE: This is a phase I study followed by a phase II study.

Beginning 48 hours before T-cell infusion, patients receive cyclophosphamide IV. Patients then receive antigen-specific CD8+ T cells IV alone or with CD4+ T helper clones over 1-2 hours on day 0. Patients also receive aldesleukin subcutaneously twice daily on days 0-13. Treatment repeats every 28 days for up to 3 courses in the absence of disease progression or unacceptable toxicity.

After completion of study treatment, patients are followed up weekly for 8 weeks, and then periodically thereafter.

Interventional
Phase 1
Phase 2
Allocation: Non-Randomized
Endpoint Classification: Safety/Efficacy Study
Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Treatment
  • Recurrent Melanoma
  • Stage IV Melanoma
  • Biological: therapeutic autologous lymphocytes
    Given IV
    Other Names:
    • AL
    • Autologous Lymphocytes
    • autologous T cells
  • Biological: aldesleukin
    Given subcutaneously
    Other Names:
    • IL-2
    • interleukin II
    • Proleukin
    • recombinant human interleukin-2
    • recombinant interleukin-2
    • TCGF, interleukin
  • Drug: cyclophosphamide
    Given IV
    Other Names:
    • CPM
    • CTX
    • Cytoxan
    • Endoxan
    • Endoxana
    • Enduxan
  • Procedure: biopsy
    Optional correlative studies
    Other Name: biopsies
  • Other: immunohistochemistry staining method
    Optional correlative studies
    Other Name: immunohistochemistry
  • Other: flow cytometry
    Correlative studies
  • Genetic: polymerase chain reaction
    Correlative studies
    Other Name: PCR
Experimental: Arm I
Beginning 48 hours before T-cell infusion, patients receive cyclophosphamide IV. Patients then receive antigen-specific CD8+ T cells IV alone or with CD4+ T helper clones over 1-2 hours on day 0. Patients also receive aldesleukin subcutaneously twice daily on days 0-13. Treatment repeats every 28 days for up to 3 courses in the absence of disease progression or unacceptable toxicity.
Interventions:
  • Biological: therapeutic autologous lymphocytes
  • Biological: aldesleukin
  • Drug: cyclophosphamide
  • Procedure: biopsy
  • Other: immunohistochemistry staining method
  • Other: flow cytometry
  • Genetic: polymerase chain reaction
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Completed
6
Not Provided
August 2010   (final data collection date for primary outcome measure)

Inclusion

  • Histopathologically documented metastatic melanoma
  • Karnofsky Performance status of at least 70%
  • Expected survival of greater than 16 weeks
  • WBC > 2,500/uL (ANC > 1,000 uL)
  • Platelet count > 80,000 uL
  • HCT > 28%
  • Patients whose tumor expresses targeted antigen and restricting allele against which CD4 and CD8 T cell clones can be generated
  • No CNS metastasis
  • Patient's whose tumor expresses an antigen and HLA type for which both an HLA Class I and HLA Class II epitope are listed, will be eligible for this study
  • CD4 and CD8 T cell clones do not necessarily have to target the same antigen to be eligible for the study, it is only necessary that the targeted antigen is expressed by the tumor and its epitope is restricted by an HLA allele expressed by the patient
  • Evidence of measurable residual disease by clinical exam or imaging studies

Exclusion

  • Current central nervous system metastases; patients with history of CNS metastases that show no current evidence of active disease are eligible
  • Patients with active infections or oral temperature > 38.2 C within 72 hours of study entry or systemic infection requiring chronic maintenance or suppressive therapy
  • Current treatment with steroids
  • Patients who are HIV seropositive (poor CD4 T cell generation due to low CD4 T cell recovery and likely HIV reservoir in stimulator cells used in vitro culture)
  • Prognosis less than 6 months
  • FOR T CELL INFUSION:
  • Pregnant women, nursing mothers of reproductive ability who are unwilling to use effective contraception or abstinence; women of childbearing potential must have a negative pregnancy test within two weeks prior to entry
  • Serum creatinine > 2.0 mg/dL
  • Significant hepatic dysfunction (hepatic toxicity >= grade 2 (NCICTC) of whatever origin
  • Clinically significant pulmonary dysfunction, as determined by medical history and physical exam; patients so identified will undergo pulmonary functions testing and those with FEV1 < 60% of normal or DLco (corr for Hgb) < 55% will be excluded
  • Significant cardiovascular abnormalities as defined by any one of the following: congestive heart failure, clinically significant hypotension, symptoms of coronary artery disease, presence of cardiac arrhythmias on EKG requiring drug therapy
  • Ejection fraction < 50% excludes patients
  • Current central nervous system metastases; patients with history of CNS metastases that show no current evidence of active disease are eligible
  • Serum calcium > 12 mg/dL
  • Chemotherapeutic agents (standard or experimental), radiation therapy, or other immunosuppressive therapies less than 4 weeks prior to T cell therapy; patients with bulky disease may undergo 1-2 courses of cytoreductive chemotherapy but treatment will be discontinued at least 4 weeks prior to T cell therapy; patients should have recovered fully from all previous treatment-related toxicities
  • History of seizures
  • Patients must not be receiving any other experimental drugs within 4 weeks of the initiation of the protocol and must have recovered from all side effects of such therapy
  • Patients with >= Grade 2 hepatotoxicity are excluded
  • Patients with a history of autoimmune disease requiring active systemic therapy are excluded
  • The following agents are not allowed while on study: systemic corticosteroids (except as outlined for management of toxicity of nontransduced CTL), immunotherapy (for example, interleukins, interferons, melanoma vaccines, intravenous immunoglobulin, expanded polyclonal TIL or LAK therapy), pentoxifylline, or other investigational agents
Both
18 Years to 75 Years
No
Contact information is only displayed when the study is recruiting subjects
United States
 
NCT00553306
2179.00, NCI-2010-01281
Yes
Yee, Cassian, Fred Hutchinson Cancer Research Center/University of Washington Cancer Consortium
Fred Hutchinson Cancer Research Center
Not Provided
Principal Investigator: Cassian Yee Fred Hutchinson Cancer Research Center/University of Washington Cancer Consortium
Fred Hutchinson Cancer Research Center
April 2011

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP