Cryosurvival of Embryos From Dysmorphic Oocytes

This study has been completed.

Sponsor:

Information provided by:

V.K.V. American Hospital, Istanbul

ClinicalTrials.gov Identifier:

NCT00521443

First received: August 24, 2007

Last updated: NA

Last verified: August 2007

History: No changes posted

Tracking Information

First Received Date ^ICMJE

August 24, 2007

Last Updated Date

August 24, 2007

Start Date ^ICMJE

Not Provided

Primary Completion Date

Not Provided

Current Primary Outcome Measures ^ICMJE

Not Provided

Original Primary Outcome Measures ^ICMJE

Not Provided

Change History

No Changes Posted

Current Secondary Outcome Measures ^ICMJE

Not Provided

Original Secondary Outcome Measures ^ICMJE

Not Provided

Current Other Outcome Measures ^ICMJE

Not Provided

Original Other Outcome Measures ^ICMJE

Not Provided

Descriptive Information

Brief Title ^ICMJE

Cryosurvival of Embryos From Dysmorphic Oocytes

Official Title ^ICMJE

Not Provided

Brief Summary

Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.

Detailed Description

Not Provided

Study Type ^ICMJE

Observational

Study Design ^ICMJE

Observational Model: Defined Population
Observational Model: Natural History
Time Perspective: Longitudinal
Time Perspective: Prospective

Target Follow-Up Duration

Not Provided

Biospecimen

Not Provided

Sampling Method

Not Provided

Study Population

Not Provided

Condition ^ICMJE

Cryopreservation
Blastocyst

Intervention ^ICMJE

Procedure: Cryopreservation, thawing and blastocyst culture

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Study Group/Cohort (s)

Control: Normal
Embryos derived from morphologically normal MII oocytes

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Irregular Shapes
Embryos derived from irregularly shaped oocytes.

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Large PVS
Embryos derived from oocytes with large perivitelline space.

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Dark Zona
Embryos derived from oocytes with dark zona pellucida

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Dark cytoplasm
Embryos derived from oocytes with dark cytoplasm

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Vacuolar cytoplasm
Embryos derived from oocytes with vacuolated cytoplasm

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Central granulation
Embryos derived from oocytes with centrally granulated cytoplasm

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Double extra
Embryos derived from oocytes with double extracytoplasmic abnormalities

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Double combined
Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Double cytoplasmic
Embryos derived from oocytes with any two cytoplasmic anomalies

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Triple extra
Embryos derived from oocytes with triple extracytoplasmic anomaly

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture
Case: Triple combined
Embryos derived from oocytes with triple combined anomalies

Intervention: Procedure: Cryopreservation, thawing and blastocyst culture

Publications *

Balaban B, Ata B, Isiklar A, Yakin K, Urman B. Severe cytoplasmic abnormalities of the oocyte decrease cryosurvival and subsequent embryonic development of cryopreserved embryos. Hum Reprod. 2008 Aug;23(8):1778-85. Epub 2008 May 12.

* Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.

Recruitment Information

Recruitment Status ^ICMJE

Completed

Enrollment ^ICMJE

Not Provided

Completion Date

Not Provided

Primary Completion Date

Not Provided

Eligibility Criteria ^ICMJE

Inclusion Criteria:

Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure.
Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle.

Exclusion Criteria:

Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture.
Embryos obtained from abnormally fertilized zygotes.

Gender

Female

Ages

18 Years to 45 Years

Accepts Healthy Volunteers

Not Provided

Contacts ^ICMJE

Contact information is only displayed when the study is recruiting subjects

Location Countries ^ICMJE

Turkey

Administrative Information

NCT Number ^ICMJE

NCT00521443

Other Study ID Numbers ^ICMJE

AH-05/04

Has Data Monitoring Committee

Not Provided

Responsible Party

Not Provided

Study Sponsor ^ICMJE

V.K.V. American Hospital, Istanbul

Collaborators ^ICMJE

Not Provided

Investigators ^ICMJE

Study Director:

Basak Balaban, B.Sc.

Assisted Reproduction Unit of the VKV American Hospital of Istanbul

Information Provided By

V.K.V. American Hospital, Istanbul

Verification Date

August 2007

^ICMJE Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP

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