Sleep Deprivation's Regulation of Immune System Function and Behavior - Full Text View

Purpose

The study proposes to investigate whether sleep deprivation will affect a variety of measures, including hormones, immune system functioning, and behaviors related to food intake and hunger. It is predicted that sleep deprivation will affect circulating neutrophil activity, and do so via affects on DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and down-regulate circulating oxytocin. Finally, it is predicted that sleep deprivation will increase participants' tendencies to pick larger portions of food, and also increase their tendency to purchase foods that are more caloric in a mock supermarket scenario.

Condition	Intervention
Sleep Deprivation Sleep	Behavioral: Neuroeconomics task Behavioral: Portion Size Task Procedure: Blood sample

Study Type:	Interventional
Study Design:	Allocation: Randomized Intervention Model: Crossover Assignment Masking: Open Label Primary Purpose: Basic Science
Official Title:	The Role of Sleep Deprivation in the Regulation of Immune System, Neuroendocrine Responses, and Behavioral Measures.

Further study details as provided by Uppsala University:

Primary Outcome Measures:

Neutrophil phagocytotic function production [ Time Frame: Change in 4 neutrophil phagocytotic function from baseline to 36hours later (after the nighttime intervention) ] [ Designated as safety issue: No ]
This project is designed to test the inflammatory capacity of circulating neutrophils. It also aims to investigate if any changes in neutrophil efficacy are governed by alterations in DNA methylation.

Measured during 36 hours (at 1930 day 1, 0730 and 1930 day 2, 0730 day 3)

Secondary Outcome Measures:

Portion Size Task [ Time Frame: Change in selected portion size from baseline to 1 hr after consuming a caloric preload ] [ Designated as safety issue: No ]
Participants will be evaluated on their tendency to choose larger or smaller portions of a variety of meal items on a computer screen. This will be conducted both following sleep deprivation and sleep, and changes over the hour will be compared between these conditions.
Neuro-economics task [ Time Frame: Change in purchasing behaviour 1 hr after consuming a caloric preload ] [ Designated as safety issue: No ]
Participants will be evaluated on their purchasing behavior with regards to high-calorie and low-calorie food items in a mock supermarket scenario following sleep and sleep deprivation.
Circulating hormone levels [ Time Frame: Change in circulating hormone levels from baseline (ie. 1930 - before sleep intervention) to 12hr later (0730 after the nighttime intervention) ] [ Designated as safety issue: No ]
Participants will have their circulating hormone levels taken and analyzed, including ghrelin and oxytocin, to determine if sleep deprivation alters hormone levels related primarily to obesity or weight gain

Enrollment:	18
Study Start Date:	February 2012
Estimated Study Completion Date:	March 2013
Estimated Primary Completion Date:	March 2013 (Final data collection date for primary outcome measure)

Arms	Assigned Interventions
Experimental: Sleep deprivation Total sleep deprivation: participants were required to stay up for the entire night before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed	Behavioral: Neuroeconomics task After a night of wakefulness, participants performed the neuroeconomics task (shopping food items in a mock supermarket scenario). Behavioral: Portion Size Task Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume Procedure: Blood sample After a night of wakefulness, a blood sample was taken to assess the level and efficacy of circulating neutrophils, as well as to assay blood serum and plasma for the presence of hormones involved in hunger such as oxytocin and ghrelin
Experimental: Sleep Sleep: participants had an 8-h sleep opportunity before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed	Behavioral: Neuroeconomics task After a night of wakefulness, participants performed the neuroeconomics task (shopping food items in a mock supermarket scenario). Behavioral: Portion Size Task Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume Procedure: Blood sample After a night of wakefulness, a blood sample was taken to assess the level and efficacy of circulating neutrophils, as well as to assay blood serum and plasma for the presence of hormones involved in hunger such as oxytocin and ghrelin

Arms

Assigned Interventions

Experimental: Sleep deprivation

Total sleep deprivation: participants were required to stay up for the entire night before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed

Behavioral: Neuroeconomics task

After a night of wakefulness, participants performed the neuroeconomics task (shopping food items in a mock supermarket scenario).

Behavioral: Portion Size Task

Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume

Procedure: Blood sample

After a night of wakefulness, a blood sample was taken to assess the level and efficacy of circulating neutrophils, as well as to assay blood serum and plasma for the presence of hormones involved in hunger such as oxytocin and ghrelin

Experimental: Sleep

Sleep: participants had an 8-h sleep opportunity before a 'Blood Sample' was taken and the 'Neuroeconomics task' and 'Portion size task' were performed

Behavioral: Neuroeconomics task

After a night of wakefulness, participants performed the neuroeconomics task (shopping food items in a mock supermarket scenario).

Behavioral: Portion Size Task

Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume

Procedure: Blood sample

After a night of wakefulness, a blood sample was taken to assess the level and efficacy of circulating neutrophils, as well as to assay blood serum and plasma for the presence of hormones involved in hunger such as oxytocin and ghrelin

Eligibility

Genders Eligible for Study:	Male
Accepts Healthy Volunteers:	Yes

Criteria

Inclusion Criteria:

Male
Age 18-35y
Healthy (self-reported) and not on medication
Non-smoking
Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported)

Exclusion Criteria:

Major illness
Taking any serious medications
Any sleep conditions (e.g. irregular bedtimes, sleep complaints)
Any dietary issues with the food items provided
A history of endocrine or psychiatric disorders

Contacts and Locations

Please refer to this study by its ClinicalTrials.gov identifier: NCT01730742

Locations

Sweden
Uppsala University
Uppsala, Sweden, 75105

Sponsors and Collaborators

Uppsala University

Investigators

Principal Investigator:

Christian Benedict, PhD

dept. of Neuroscience, Uppsala University

More Information

No publications provided

Responsible Party:	Uppsala University
ClinicalTrials.gov Identifier:	NCT01730742 History of Changes
Other Study ID Numbers:	192837465
Study First Received:	October 11, 2012
Last Updated:	November 15, 2012
Health Authority:	Sweden: Regional Ethical Review Board

Additional relevant MeSH terms:

Sleep Deprivation
Dyssomnias
Sleep Disorders
Nervous System Diseases

Neurologic Manifestations
Signs and Symptoms
Mental Disorders

ClinicalTrials.gov processed this record on May 19, 2013

Sleep Deprivation's Regulation of Immune System Function and Behavior (SS)