Isolated Erythrocyte Membrane Susceptibility to Photo-oxidative Stress in Alzheimer's Disease
The study hypothesis is that high lipid peroxidation and decreased antioxidant defenses characterize the natural history of Alzheimer's disease.
The purpose of this study is to investigate susceptibility to photo-oxidation of isolated erythrocyte membranes, in patients affected by Alzheimer's disease and age- and sex-matched, non demented subjects.
It will be evaluated the release of malondialdehyde (MDA) from photo-oxidized erythrocyte ghosts, through a very easy and convenient procedure for the preparation of erythrocyte membrane samples.
Induction of oxidative stress through ultraviolet rays, unlike that obtained by chemical oxidizing agents, has the advantage to be fully controllable, since it produces effects only during irradiation. Moreover, use of isolated erythrocyte membranes allows for greater specificity in the evaluation of MDA produced, and reduces the amount of blood required for the assay.
|Study Design:||Observational Model: Case Control
Time Perspective: Cross-Sectional
|Official Title:||Isolated Erythrocyte Membrane Susceptibility to Photo-oxidative Stress in Patients Affected by Alzheimer's Disease and Healthy Controls|
- Malondialdehyde assay [ Time Frame: At the time of recruitment ] [ Designated as safety issue: No ]Isolated and purified red blood cell membranes will be in vitro exposed to oxidative stress by UV-B radiation. The extent of cell membrane damage will be quantified by the fluorometric determination of malondialdehyde.
- Relationship between urinary excretion of cortisol and levels of malondialdehyde [ Time Frame: At the time of recruitment ] [ Designated as safety issue: No ]Hyperactivity of the hypothalamic pituitary adrenal axis has been frequently described in Alzheimer's disease. Recently published work reported an association between high secretion of cortisol and oxidative stress. We will investigate the relationship between 24 h excretion of urinary cortisol and the level of malondialdehyde, produced by isolated and purified red blood cell membranes, in vitro exposed to oxidative stress by UV-B radiation.
Biospecimen Retention: Samples With DNA
|Study Start Date:||October 2012|
|Estimated Study Completion Date:||October 2013|
|Estimated Primary Completion Date:||April 2013 (Final data collection date for primary outcome measure)|
|Non demented subjects|
Please refer to this study by its ClinicalTrials.gov identifier: NCT01707719
|Contact: Raffaele Antonelli Incalzi, M.D.||+email@example.com|
|Policlinico Universitario Campus Bio-Medico||Not yet recruiting|
|Rome, RM, Italy, 00128|
|Contact: Raffaele Antonelli Incalzi, M.D. +39-06-225411 firstname.lastname@example.org|
|Contact: Francesco Maria Serino, M.D. +39-06-225411 ext 9123 email@example.com|
|Principal Investigator:||Francesco Maria Serino, M.D.||University Campus Bio-Medico|
|Principal Investigator:||Chiara Fanali, PhD||University Campus Bio-Medico|
|Principal Investigator:||Laura Dugo, PhD||University Campus Bio-Medico|
|Principal Investigator:||Simone Grasso, PhD||University Campus Bio-Medico|
|Study Chair:||Ettore Bergamini, M.D.||University of Pisa|
|Principal Investigator:||Francesca Ursini, M.D.||University Campus Bio-Medico|
|Principal Investigator:||Fabrizio Vernieri, M.D.||University Campus Bio-Medico|
|Study Director:||Marina Dachà, BS.Pharm||University Campus Bio-Medico|