Genetic Determinant of Blood Pressure Sensitivity to Salt Intake (JEU004)
Nedd4.2 is a regulator of ENac, a transporter of sodium involved in the regulation of salt retention by the kidney. The study address the consequence of a frequent genetic variation leading expected to impact the function of Nedd4 and in turn, the ability of kidneys to excrete salt intake. Our hypothesis is that the genetic variants of Nedd4.2 could constitute a genetic predisposition to (or protection against) salt-sensitive hypertension.
|Study Design:||Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Basic Science
|Official Title:||Clinical, Pharmacological and Biological Investigations of a Polymorphism of Nedd4 , a Regulatory Protein of the Epithelial Sodium Channel|
- Difference in Blood Pressure among genotypes [ Time Frame: 24h AMBP will be measured weekly for the duration of study, an expected average of 3 weeks ] [ Designated as safety issue: No ]24h AMBP will be measured weekly for the duration of study, an expected average of 3 weeks
- Ambulatory Blood Pressure (AMBP) [ Time Frame: three weeks ] [ Designated as safety issue: No ]24h AMBP was measured fourth : at the beginning of the study,at the end of the low sodium-high potassium diet, after one week under the high sodium-low potassium diet and at the end of amiloride treatment under high sodium-low potassium diet
|Study Start Date:||April 2007|
|Study Completion Date:||March 2012|
|Primary Completion Date:||March 2012 (Final data collection date for primary outcome measure)|
Experimental: Diet Amiloride
One week Low Na diet (10 mmol/d) followed by ine week high Na diet (200 mmol/d) followed by 2 weeks administration of amiloride (10 mg the 1 st week and the 20mg).
Other: Diet Amiloride
only arm of the trial
Other Name: Diet Amiloride
Subjects were submitted one week to a low sodium-high potassium diet, and then switch to a high sodium,-low potassium diet for two weeks. the last week, subject were taken a 20 MG daily dose of amiloride, a potassium sparing diuretic.