Analysis of Fel d 1-specific T Cells After Airway Allergen Challenge in Asthma
Cat allergies are a major trigger of asthma. Therapies are being developed to control the allergic response to cats. We are interested in measuring a type of white blood cell which is linked to cat allergies, which will help us understand how to use new therapies in people who suffer from cat allergies and asthma. We will study cat-allergic individuals with stable, mild asthma who will be exposed to cat allergens. We will measure various white blood cells, including the cells that are linked to cat allergies, to determine whether the number of these cells changes following cat exposure. These cells will be measured from the blood and bone marrow by removing samples using a needle. These cells will also be measured from the lungs by inserting a bronchoscope into the airways and drawing up fluid containing cells. This study will improve our understanding of the harmful versus protective role of these cat-specific cells, and will allow for development of better drugs for treatment of asthma triggered by cat exposure.
|Study Design:||Observational Model: Case-Only
Time Perspective: Prospective
|Official Title:||Analysis of Fel d 1-specific T Cells After Airway Allergen Challenge in Asthma:Frequency and Phenotype and Trafficking of Cat Allergen-specific T-cells in Blood, Bone Marrow and Bronchoalveolar Lavage Fluid (BALF) Following Segmental Allergen Challenge in Allergic Asthma.|
- Comparison [ Time Frame: 5 Years ] [ Designated as safety issue: No ]The primary outcome for Aim 1 of the study is to compare the number of tetramer+ T cells in the airways of early and dual-responder subjects after segmental allergen challenge. The primary outcome for Aim 2 of the study is to compare the number of tetramer+ T cells in the bone marrow of early and dual-responder subjects after segmental allergen challenge. This will be measured using a panel of antibodies and flow cytometric analysis of cell surface markers.
- Measurements [ Time Frame: 5 Years ] [ Designated as safety issue: No ]The secondary outcome is to determine the frequency, memory phenotype and chemokine receptor profile of tetramer+ T cells in the peripheral blood before and after bronchial allergen challenge.
|Study Start Date:||June 2012|
|Estimated Study Completion Date:||May 2017|
Mild asthma, cat-allergic, 18-65 years old, males and females will be recruited for the study.
Other: Segmental Allergen Challenge
segmental allergen challenge
|Contact: Gail Gauvreau, PhD||905-525-9140 ext firstname.lastname@example.org|
|McMaster University Medical Centre||Not yet recruiting|
|Hamilton, Ontario, Canada, L8S 4K1|
|Contact: Gail Gauvreau, PhD 905-525-9140 ext 22791 email@example.com|
|Sub-Investigator: Paul O'Byrne, MD|
|Sub-Investigator: Kieran Killian, MD|
|Sub-Investigator: Helen Neighbour, MD|
|Sub-Investigator: Mark Larche, PhD|
|Principal Investigator:||Gail Gauvreau, PhD||McMaster University|