Full4Health: Understanding Food-gut-brain Axis Across the Lifecourse (F4H)
The primary aim of this work is, to 'relate psychological and behavioural parameters of hunger/satiety and food preference to gut hormones, neural activation and energy metabolism by dietary manipulation, across the human lifespan'.
|Study Design:||Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double Blind (Subject, Caregiver, Investigator, Outcomes Assessor)
Primary Purpose: Basic Science
|Official Title:||Full4Health: Neuro-gut Interactions in Humans Across the Lifecourse|
- Changes in concentrations of biomarkers of appetite in response to each milk-based beverage [ Time Frame: During each of the four 'Breakfast Study' visits blood will be taken at baseline, and 30, 60, and 120 minutes after consuming the milk-based beverage. Each visit will be seperated by one week. ] [ Designated as safety issue: No ]
The following blood-borne biomarkers of appetite will be measured:
- Total cholesterol
- Low density lipoprotein
- High density lipoprotein
- Ghrelin (active)
- GLP-1 (active)
- GIP (total)
- Peptide YY (total)
- Amylin (active)
- Cholecystokinin (CCK)
- Neural responses to images of food when fasted and after consuming a milk-based beverage [ Time Frame: There will be one week between both conditions (fasted and fed) ] [ Designated as safety issue: No ]The subjects will conduct a computerised task that will be performed in the functional magnetic resonance imaging (fMRI) scanner. Subjects will be measured twice, on separate days, either after an overnight fast or after a test meal, fed to satiation.
|Study Start Date:||May 2012|
|Estimated Study Completion Date:||December 2014|
|Estimated Primary Completion Date:||August 2014 (Final data collection date for primary outcome measure)|
|Experimental: Phase 1: Breakfast Study||
Other: Breakfast Study
Subjects (male and female, lean and obese, children, teenagers, adults, and elderly) will take part in 4 morning sessions, consuming a test breakfast milk based beverage and assess appetite, biomarkers in blood and saliva and complete questionnaires to assess influences on feeding behaviour. We will also collect a single saliva sample from each participant to examine genetic traits related to appetite, food choice, body weight, and energy expenditure. There are two milk based beverages, one protein enriched (30% protein from calories) and one normal protein (15% protein). Subjects will be offered a morning snack buffet to assess ad libitum feeding behaviour. Phase 1 will also include a subgroup of malnourished male and female elderly participants.
|Experimental: Phase 2: fMRI Study||
Other: fMRI Study
We will fMRI scan normal weight and overweight subjects of both gender from the four different age groups only: 8-10, 13-17, 24-45 and 65-75 years. Subjects will be measured twice, on separate days, either after an overnight fast or after a test meal, fed to satiation (because hunger will modulate the response to food presentation). The subjects will conduct a computerised task that will be performed in the scanner to assess hedonic responses to food cues. Physiological biomarkers will be measured during both trials for the assessment of appetite hormone circulation. Saliva samples will be taken for DNA analysis. DNA extraction techniques will be used to examine genetic traits linked to appetite, food choice, body weight, and energy expenditure.
The Full4Health project aims to further understanding of the mechanisms of hunger and satiety. The proposal integrates investigation of human volunteers and laboratory rodents throughout the life course, applying imaging and other cutting edge technologies to critical research questions. Full4Health will combine study of the mechanisms of hunger and satiety with intervention studies to validate the effects of the relevant food characteristics on the regulation of satiety/hunger. The development of cerebral responses to food through the gut-brain axis across lifespan particularly during childhood, adolescence and elderly will be studied.
|Contact: Alexandra M Johnstone, PhD||01224438614 ext email@example.com|
|Contact: William Buosi, MSc||01224438691 ext firstname.lastname@example.org|
|Harokopia Univeristy||Active, not recruiting|
|The Rowett Institute of Nutrition and Health, University of Aberdeen||Recruiting|
|Aberdeen, Aberdeenshire, United Kingdom, AB21 9SB|
|Principal Investigator: Alexandra M Johnstone, PhD|
|Sub-Investigator: Daniel Crabree, MSc|
|Sub-Investigator: William Buosi, MSc|
|Principal Investigator:||Alexandra M Johnstone, PhD||The Rowett Institute of Nutrition and Health, University of Aberdeen|