Studying Genes in Tissue Samples From Younger and Adolescent Patients With Soft Tissue Sarcomas
RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors find better ways to treat cancer.
PURPOSE: This research trial studies genes in tissue samples from younger and adolescent patients with soft tissue sarcomas.
Genetic: DNA analysis
Genetic: microarray analysis
Genetic: polymerase chain reaction
Other: laboratory biomarker analysis
Other: mass spectrometry
|Official Title:||Potentially Actionable Mutations in Archived Non-Rhabdomyosarcoma Soft Tissue Sarcomas (NRSTS)|
- Frequency of individual mutated genes in patients with NRSTS [ Designated as safety issue: No ]
- Genetic changes that are most common and likely to have the greatest therapeutic impact [ Designated as safety issue: No ]
|Study Start Date:||March 2012|
|Estimated Primary Completion Date:||May 2012 (Final data collection date for primary outcome measure)|
- To determine the frequency with which actionable mutations are found in archived non-rhabdomyosarcoma soft tissue sarcoma (NRSTS) tumor specimens using mass spectrometry (MS) analysis of tumor-derived deoxyribonucleic acid (DNA).
OUTLINE: Archived DNA tissue samples are analyzed for frequency of genetic mutations, including single nucleotide polymorphisms (SNPs), single nucleotide variants (SNVs), and small deletions and/or insertions, by polymerase chain reaction (PCR) and mass spectometry (Sequenom MassARRAY). Results are then analyzed to determine whether specific mutations correlate with patient or disease features such as tumor stage, histological grade, or outcome.
|Principal Investigator:||Steve Skapek, MD||University of Texas Southwestern Medical Center at Dallas|